Leaf anatomy and histochemistry of Macropeplus dentatus (Perkins) I. Santos & Peixoto and Macropeplus ligustrinus (Tul.) Perkins (Monimiaceae)

Abstract

RESUMO -(Anatomia e histoquímica foliar de Macropeplus dentatus (Perkins) I. Santos & Peixoto e Macropeplus ligustrinus (Tul.) Perkins (Monimiaceae)). A família Monimiaceae está representada na região Neotropical por seis gêneros. Macropeplus Perkins é um gênero, exclusivamente brasileiro, constituído por quatro espécies restritas às Florestas Alto-Montanas e que carecem de estudos anatômicos. O presente trabalho visa caracterizar a estrutura anatômica e a histoquímica da folha de Macropeplus ligustrinus (Tul.) Perkins e Macropeplus dentatus (Perkins) I. Santos & Peixoto, a fi m de ampliar o conhecimento sobre esses táxons e auxiliar na taxonomia do gênero. Os indivíduos amostrados estão localizados no Parque Nacional da Serra dos Órgãos (RJ) e do Parque Natural do Caraça (MG). Dentre as características observadas comuns às espécies destacam-se: estrutura dorsiventral, folha hipoestomática, estômatos paracíticos, estrato subepidérmico, sistema vascular colateral em forma de arco no pecíolo, padrão de venação do tipo semicraspedobroquidódro-mo e presença de cristais prismáticos. Alguns metabólitos especiais foram identifi cados como substâncias fenólicas, lipídios e mucilagem, sendo o último detectado pela primeira vez na família. O contorno do pecíolo, a ocorrência e distribuição das células secretoras e das braquiesclereídes, em associação com os dados morfológicos, são úteis na distinção dessas espécies. Palavras-chave: anatomia foliar, histoquímica, Macropeplus, Mata Atlântica, Monimiaceae ABSTRACT -(Leaf anatomy and histochemistry of Macropeplus dentatus (Perkins) I. Santos & Peixoto and Macropeplus ligustrinus (Tul.) Perkins (Monimiaceae)). The Monimiaceae is represented in the Neotropical region by six genera. Macropeplus Perkins is a Brazilian genus comprising four species restricted to high-montane Atlantic Forest areas, which have not been properly examined from the anatomical point of view. This study characterizes the anatomical structure and histochemical aspects of the leaves of Macropeplus dentatus and M. ligustrinus, and adds to our taxonomic knowledge of the genus. The individuals studied were collected in the Serra dos Órgãos National Park and in the Caraça Natural Park in southeastern Brazil. Among the characteristics common to the two species, hypostomatic leaves, dorsiventral structure, paracytic stomata, a subepidermal layer, collateral vascular system in the form of an arc in the petiole, semicraspedobrochidodromous venation, and the presence of prismatic crystals were registered. Intracellular metabolites, such as phenolic compounds, lipids and mucilage, were identifi ed. The petiole shape, occurrence and distribution of secretory cells and brachysclereids, in association with other morphological features, were relevant to species identifi cation.

Hedycaryamacrofossils and associatedPlanarpollenitespollen from the early Miocene of New Zealand

“…5D-F ), and Renner et al (2010) placed Hedycarya , Levieria , and Kibaropsis in the same molecular clade. Costa et al (2010) described the leaf epidermis of Macropeplus J.R.Perkins as having slightly curved anticlinal cell walls, noting that it was similar to most previously described members the family ( Peixoto, 1979 ;Barrera and Meza, 1992 ;Barros et al, 1997 ). Stomata are anomocytic in Palmeria F.Muell.…”

“…( Metcalfe, 1987 ) and Peumus ( Fig. 5G-I ; Barrera and Meza, 1992 ), but paracytic in Macropeplus ( Costa et al, 2010 ) and Mollinedia Ruíz & Pav. ( Peixoto, 1979 ; TABLE 3.…”

Taxonomic relevance of leaf anatomy in Banisteriopsis C.B. Rob. (Malpighiaceae)

“…Petiole anatomical characters are regarded as useful to distinguish taxa due to its stability in response to different environmental variables (Metcalfe & Chalk 1979). This character has successfully been applied to comparative anatomical studies in several plant families (Rio et al 2005;Costa et al 2010;Moraes et al 2011;Ogundare & Saheed 2012;Almeida Jr. et al 2013;Coutinho et al 2013). Previously, only two types of petiole contour were reported in the literature for Malpighiaceae, concave-convex, and plane-convex (Araújo et al 2010).…”

Anatomy and Histochemistry of Leaf and Stem of Brazilian Endemic Species Mollinedia clavigera Tul.

“…Secretory cells are frequently found in epidermis and mesophyll of Monimiaceae species [21], such as Macropeplus dentatus (Perkins) I. Santos & Peixoto and M. ligustrinus (Tul.) Perkins [31]. The presence or absence of oil cells may assist in species identification [32].…”