Proteinograma sérico, com ênfase em proteínas de fase aguda, de bovinos sadios e bovinos portadores de enfermidade aguda de ocorrência natural

Simplício, Kalina M.M.G.; Sousa, Filipe; Fagliari, José Jurandir; Silva, P C

doi:10.1590/s0102-09352013000500011

Cited by 3 publications

(4 citation statements)

References 15 publications

(6 reference statements)

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“…The serum electrophoretic profile values obtained were similar to those described in the literature for healthy cattle of the same age range (Fagliari et al 2007, Simplício et al 2013, Sampaio et al 2015. The CSF profile has not yet been determined for bovines, so no comparative data are available.…”

Section: Discussionsupporting

confidence: 78%

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

et al. 2018

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Use of acute-phase proteins (APPs) for assessment of health and disease in animals has increased greatly within the last decade. The objective was to determine the normal concentration of APPs in the serum and cerebrospinal fluid (CSF) of healthy cattle by polyacrylamide gel electrophoresis. Fifty crossbred animals (350±70kg of BW and 18±1.2 months of age), 25 heifers and 25 steers were used. CSF samples were collected from atlanto-occipital (AO) site and blood samples were obtained from the jugular vein. CSF and serum protein electrophoresis were performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thirty-seven proteins with molecular weights ranging from 7 and 37kDa were identified in CSF of all animals. These eight were nominally identified with immunoglobulin A and G, celuloplasmin, transferrin, albumin, α1-antitripsin, acidic glycoprotein, and haptoglobin. All protein fractions in CSF did not differ between heifers and steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, but only the CSF presented α1-antitripsin. The serum values of acidic glycoprotein and immunoglobulin G were significantly higher in steers compared with heifers. In conclusion, measurement of CSF acute phase protein concentrations can be useful in diagnosing and monitoring the progression of bovine neurological diseases, perhaps even to guide therapeutic procedures. The CSF electrophoretic profile of healthy cattle does not change depending on gender.

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Section: Discussionsupporting

confidence: 78%

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

et al. 2018

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“…The APP have different species responsiveness based on the inflammatory stimulus that has settled, becoming in the last two decades the biomarkers of choice in human medicine and, by extension, in veterinary medicine. Acid α1-glycoprotein was considered effective as a biomarker for early diagnosis in monitoring the prognosis of sepsis [31], such potential has been proved in cattle with mastitis and photosensitization [27]. Moreover, they demonstrated the sensitivity of ceruloplasmin and haptoglobin in cattle with mastitis, photosensitization and onfalophlebitis.…”

Section: Discussionmentioning

confidence: 99%

“…Monitoring the inflammatory response is a challenge because the signs of inflammation are not always clear [27]. Hemogasometry is an important technique for assessing acid-base balance in various diseases, capable of clarifying severity and predicting clinical evolution [15,26,28].…”

Section: Introductionmentioning

confidence: 99%

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Predictive Elements of Obstructive Urolithiasis in Sheep*

Maciel

Oliveira

Afonso

et al. 2019

Acta Scientiae. Vet.

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Background: Urolithiasis is an economically important disease that has considerable significance for sheep farming. With the tissue and biochemical changes resulting from the development of this disease, metabolic disorders and immune response are established. Hemogasometric evaluation allows the identification of systemic acid-base imbalances quickly. Acute phase proteins (AFP) have in the last two decades become the biomarkers of choice in human and veterinary medicine. To date, no biomarker studies have been published for sheep with obstructive urolithiasis. Thus, this study aimed to analyze the hemogasometric kinetics in obstructive urolithiasis in sheep and the AFP that can be used as early biomarkers in this disease.Materials, Methods & Results: In this study, 14 healthy male Santa Inês sheep, aged approximately 90 days, fed on calculogenic diet for 120 days. The animals were examined weekly, including observation of clinical signs, and blood and urine analysis were performed. For comparative analysis purposes, at the end of the experiment, sheep that developed obstructive urolithiasis were extracted from the initial experimental group D1 (without urolithiasis) and became part of the second experimental group D2 (with urolithiasis). In the pre-experimental period and on the day of slaughter, venous blood samples were harvested for hemogasometric tests, with a maximum time of 15 minutes between collection and analysis, to ensure the reliability of the results obtained. The pH, pCO2, pO2, EB, tCO2, HCO3-, stHCO3-, tHb, sO2 and Hct, Na+, K+ and Ca2+ ions were quantified. To identify and measure immunoglobulins (A and G) and AFP, samples from sheep that developed obstructive urolithiasis (D2) were analyzed. Blood samples were harvested weekly until the clinical manifestation of the disease, totaling 16 samples, when IgA, IgG, ceruloplasmin, transferrin, albumin, α1-antitrypsin, haptoglobin and α1-acid glycoprotein concentrations were measured. Elevation of pCO2 was observed between D1 and D2, but there was a significant difference (P < 0.05) only in the final moments (FMs). Although EB, tCO2, HCO3-, stHCO3- increased between moments in the same group and between groups at the same time, significant differences were recorded only in the FMs. Higher values were observed for Na+, K+ in the FMs. The AFP of sheep that developed the disease oscillated between moments, however, significant difference (P < 0.05) over time was observed only in haptoglobin and transferrin.Discussion: The disease occurred in five of the 14 animals studied, demonstrating the effectiveness of the formulated diet in inducing the disease. Through the analysis of blood gases, plasma bicarbonate concentration and excess base or deficit it was possible to diagnose disturbances in acid base balance, characterizing a picture of metabolic alkalosis in animals with urolithiasis. Mean pH was not significantly different between groups, but sheep that developed urolithiasis had alkalosis. Final values of tCO2 and HCO3- indicate the compensatory organic response, which, together with the analysis of the averages of HCO3- and EB, reflect the metabolic alkalosis picture. The AFP have different responsiveness among them. Haptoglobin and transferrin were the most reliable biomarkers among the studied AFP to predict obstructive urolithiasis, with transferrin showing atypical behavior, characteristic of positive AFP.

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Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis

Santos

Rego

Afonso

et al. 2021

Semina: Ciênc. Agrár.

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This study aimed to evaluate the hematologic response and the serum and peritoneal fluid (PF) proteinogram of cattle affected by digestive diseases. Twenty-seven animals were distributed in two groups: GI (intestinal diseases) and GII (traumatic reticuloperitonitis, TRP). The animals were previously submitted to a physical exam. Subsequently, blood samples were collected to perform the complete blood count, determine the plasma protein and fibrinogen, and obtain the serum for proteinogram in polyacrylamide gel (SDS-PAGE). Simultaneously, PF was collected to perform physical and chemical evaluation and the electrophoretic profile (SDS-PAGE). ANOVA at the 5% probability level was used to compare the groups. The animals showed signs of apathy, dehydration, and gastrointestinal hypomotility in both groups. However, GI animals showed more significant clinical changes. The blood count of both groups (P > 0.05) showed leukocytosis due to neutrophilia and a regenerative left shift with hyperfibrinogenemia. The proteinogram of both body fluids allowed the identification of proteins albumin (ALB), transferrin (TRF), ceruloplasmin, haptoglobin, ?1-acid glycoprotein (?1-AGP), MW 23000 Da, ?1-antitrypsin, IgA, and IgG. The [PT] PF/[PT] blood serum ratio of each of the identified proteins increased, showing statistical differences between groups (P < 0.05) regarding PT, ALB, TRF, ?1-AGP, and IgG values, with GI animals showing the highest ratio. Intestinal diseases and TRP triggered a systemic and local response characterized by clinical, hematological, and serum and PF proteinogram alterations. The proteins ?1-GPA, haptoglobin, and TRF measured in PF were good inflammation biomarkers and useful as an auxiliary tool for the diagnosis and prognosis of digestive diseases in cattle.

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Proteinograma sérico, com ênfase em proteínas de fase aguda, de bovinos sadios e bovinos portadores de enfermidade aguda de ocorrência natural

Cited by 3 publications

References 15 publications

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

Predictive Elements of Obstructive Urolithiasis in Sheep*

Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis

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