Efficacy of piperine in reducing the effects of aflatoxin intoxication in broiler chickens: a preliminary report

Cardoso, Verônica da Silva; Castro, I. S.; Lima, Cristina Amorim Ribeiro de; Lima, Marco Edílson Freire de; Dorneles, Luis Eduardo Gomes; Direito, Glória Maria; Danelli, M.G.M.

doi:10.1590/s0102-09352011000200031

Cited by 9 publications

(5 citation statements)

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“…We observed megalocytosis, mononuclear cell infiltrate regions, congestion of epithelial cells of the bile duct, necrosis of biliary ducts, vacuolar degeneration, edema and diffuse vacuolation of hepatocytes. These effects were not found in animals treated with piperine [44]. Cardoso et al [31] evaluated the effect of piperine in different concentrations (60, 120, and 180 mg·kg −1 ) for 35 consecutive days and the best and least toxic concentration was 60 mg·kg −1 .…”

Section: Resultsmentioning

confidence: 99%

“…The aflatoxin was given to broilers which were weighed daily. The doses of piperine and AFB1 used were in accordance with Cardoso et al [31,44]. …”

Section: Methodsmentioning

confidence: 99%

“…Previous studies by our group have demonstrated that piperine intake decreased the severity of the toxic effects of aflatoxins in broiler chickens, preventing the development of liver injury and the adverse effects on hematological parameters, which are characteristic of aflatoxicosis. These data suggest that piperine might be used to prevent the effects of aflatoxin ingestion [44]. The poultry industry seeks the development of a feed additive with a natural, non-toxic substance such as piperine not only to promote the improvement of poultry health but also to contribute to increased productivity in this industry.…”

Section: Introductionmentioning

confidence: 99%

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Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1

Cardoso

Vermelho

Lima

et al. 2016

Toxins

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Piperine is an abundant amide extracted from black pepper seeds which has been shown to have protective effects against cytotoxic and genotoxic carcinogenesis induced by certain chemical carcinogens and aflatoxin B1 (AFB1) in vitro. The aim of this work was to study, in vivo, the antigenotoxic potential of feed-added piperine on broiler chickens experimentally intoxicated with AFB1, using micronucleus and comet assays. The antigenotoxicity assessment of 9-day-old chicks was performed on a total of 60 chickens divided into four groups of 15 broilers each: (C) control, (P) 60 mg·piperine kg−1 feed, (A) 0.5 mg·AFB1·kg−1 body weight, (daily by oral route), and (P + A) co-treatment with piperine and AFB1. The experiment was conducted for 26 days. Chicks intoxicated with AFB1 showed significant genotoxic effects in the first 24 h post intoxication, and the effects remained in the other periods analyzed (48, 72, and 96 h and 26 days of treatment). The DNA damage in peripheral blood cells, the number of erythrocytes with micronuclei, and polychromatic-to-normochromatic erythrocyte ratio were significantly reduced or absent in the piperine/AFB1 group. No significant differences were observed between the group piperine/AFB1 and the control and piperine-alone groups. The addition 60 mg·kg−1 of piperine to the diet of the broiler chicks was safe, promoting beneficial effects in poultry health with respect to the toxic effects 0.5 mg·AFB1·kg−1 body weight.

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Section: Resultsmentioning

confidence: 99%

“…The aflatoxin was given to broilers which were weighed daily. The doses of piperine and AFB1 used were in accordance with Cardoso et al [31,44]. …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1

Cardoso

Vermelho

Lima

et al. 2016

Toxins

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“…In another study, Dimitri et al 40 reported that exposure to AFB 1 and AFG 2 caused an average of 539 g (16%) body weight loss in rabbits, and this was related to impaired protein synthesis and DNA synthesis. Cardoso et al 41 stated that the mixture containing 62.9% of AFB 1 and 37.1% of AFG 1 significantly decreased the body weight gain in broiler chicks, did not cause a change in liver anatomy in terms of shape, texture and color, however, it caused an increase in relative liver weight.…”

Section: Resultsmentioning

confidence: 99%

Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice

Gündüz

Yalçın

Çavuşoğlu

2021

Sci Rep

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In this study, the toxic effects of aflatoxin B2 (AFB2) on Swiss albino mice and the protective effects of resveratrol were investigated. Physiological (body weight, liver and kidney weight), biochemical (aspartate aminotransferase-AST, alanine transaminase-ALT, blood urea nitrogen-BUN, creatinine, malondialdehyde-MDA and glutathione-GSH) and cytogenetic parameters (micronucleus-MN in buccal epithelium, erythrocyte and leukocyte cells and chromosomal aberrations-CAs) were used to determine the toxic effects. Additionally, scavenging effects of resveratrol against superoxide, hydrogen peroxide (H2O2) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were also investigated. In experimental period, mice were divided into six groups and the groups were treated with tap water, 10 mg/kg b.w resveratrol, 20 mg/kg b.w resveratrol, 20 µg/kg b.w. AFB2, 10 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, 20 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, respectively. As a result, the scavenging effects of resveratrol increased with increasing dose and the superoxide, H2O2 and DPPH radical scavenging activity of resveratrol were 74.9%, 79.1% and 49.2%, respectively. AFB2 administration caused a significant decrease in physiological parameters, and these decreases regressed in AFB2 + resveratrol treated groups. Serum ALT and AST activities, BUN and creatinine levels were higher in the AFB2 treated group compared to the control group and serious abnormalities were found in MDA and GSH levels in the kidney and liver. In the group treated with AFB2 + 20 mg/kg resveratrol, ALT, AST, BUN and creatinine levels decreased significantly and GSH levels increased compared to only-AFB2 treated group. AFB2 triggered MN formation in buccal epithelium, erythrocyte and leukocyte cells and CAs in bone marrow cells. The application of 20 mg/kg resveratrol together with AFB2 was decreased the MN and CAs frequency. Resveratrol exhibited a recovery effect in the range of 40.9–80.5% against AFB2 toxicity in all tested parameters. In this study, it was determined that AFB2 caused serious changes in selected physiological, biochemical and cytogenetic parameters while resveratrol displayed a protective role against these toxic effects.

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“…The aflatoxins B 1 , B 2 , G 1 and G 2 produced by Aspergillus flavus and A. parasiticus are considered the most relevant group in Brazil due to their high incidence and the toxic effects they have on humans and animals (Arana et al 2011;Cardoso et al 2011;Hoeltz et al 2012). When ingested by an animal, AFB 1 is biotransformed in the liver and excreted in the milk as AFM 1 (Hussein and Brasel 2001).…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil

Trombete

Castro

Teixeira

et al. 2013

Braz. arch. biol. technol.

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Efficacy of piperine in reducing the effects of aflatoxin intoxication in broiler chickens: a preliminary report

Abstract: Communication

Cited by 9 publications

References 10 publications

Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1

Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1

Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice

Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil

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