Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice

Abstract

In this study, the toxic effects of aflatoxin B2 (AFB2) on Swiss albino mice and the protective effects of resveratrol were investigated. Physiological (body weight, liver and kidney weight), biochemical (aspartate aminotransferase-AST, alanine transaminase-ALT, blood urea nitrogen-BUN, creatinine, malondialdehyde-MDA and glutathione-GSH) and cytogenetic parameters (micronucleus-MN in buccal epithelium, erythrocyte and leukocyte cells and chromosomal aberrations-CAs) were used to determine the toxic effects. Additionally, scavenging effects of resveratrol against superoxide, hydrogen peroxide (H2O2) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were also investigated. In experimental period, mice were divided into six groups and the groups were treated with tap water, 10 mg/kg b.w resveratrol, 20 mg/kg b.w resveratrol, 20 µg/kg b.w. AFB2, 10 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, 20 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, respectively. As a result, the scavenging effects of resveratrol increased with increasing dose and the superoxide, H2O2 and DPPH radical scavenging activity of resveratrol were 74.9%, 79.1% and 49.2%, respectively. AFB2 administration caused a significant decrease in physiological parameters, and these decreases regressed in AFB2 + resveratrol treated groups. Serum ALT and AST activities, BUN and creatinine levels were higher in the AFB2 treated group compared to the control group and serious abnormalities were found in MDA and GSH levels in the kidney and liver. In the group treated with AFB2 + 20 mg/kg resveratrol, ALT, AST, BUN and creatinine levels decreased significantly and GSH levels increased compared to only-AFB2 treated group. AFB2 triggered MN formation in buccal epithelium, erythrocyte and leukocyte cells and CAs in bone marrow cells. The application of 20 mg/kg resveratrol together with AFB2 was decreased the MN and CAs frequency. Resveratrol exhibited a recovery effect in the range of 40.9–80.5% against AFB2 toxicity in all tested parameters. In this study, it was determined that AFB2 caused serious changes in selected physiological, biochemical and cytogenetic parameters while resveratrol displayed a protective role against these toxic effects.

Molecular docking assisted biological functions and phytochemical screening of Amaranthus lividus L. extract

“…The absorbance of the solution was determined spectrophotometrically at 510 nm after 15 min incubation. Quercetin was used as the standard and flavonoid amounts were expressed as mg QE/100 mg dry weight 12 .…”

“…The radical scavenging effect of A. lividus extract was tested against superoxide and 2,2-diphenyl-1-picrylhydrazy (DPPH) radicals. Superoxide radical scavenging activity and DPPH removal activity was carried out according to the method suggested by Gündüz et al 12 . BHT was used as a standard in both radical scavenging activities.…”

Resveratrol Relieved Acute Liver Damage in Ducks (Anas platyrhynchos) Induced by AFB1 via Modulation of Apoptosis and Nrf2 Signaling Pathways

“…The supplementation of 1% Res significantly increased the contents of T-AOC, CAT and SOD in the plasma of broilers [ 26 ]. Studies have shown that Res can protect mice from AFB2 injury, mainly in relation to the scavenging activity of superoxide, H 2 O 2 and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, and the antioxidant effect [ 33 ]. Our findings demonstrated that AFB1 led to significant oxidative damage and Res reversed the trend, as shown with the decrease in GSH, GSH-ST, T-AOC, CAT and SOD levels decrease and the increase in H 2 O 2 and MDA levels.…”

Investigation of cypermethrin toxicity in Swiss albino mice with physiological, genetic and biochemical approaches

“…NO: B549-150) and creatinine (CAT. NO: C513-480) levels were measured with the help of Medispec 99 M autoanalyzer using commercially available kits (Teco Diagnostics, USA) 10 , 11 .…”