Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1

Abstract

Piperine is an abundant amide extracted from black pepper seeds which has been shown to have protective effects against cytotoxic and genotoxic carcinogenesis induced by certain chemical carcinogens and aflatoxin B1 (AFB1) in vitro. The aim of this work was to study, in vivo, the antigenotoxic potential of feed-added piperine on broiler chickens experimentally intoxicated with AFB1, using micronucleus and comet assays. The antigenotoxicity assessment of 9-day-old chicks was performed on a total of 60 chickens divided into four groups of 15 broilers each: (C) control, (P) 60 mg·piperine kg−1 feed, (A) 0.5 mg·AFB1·kg−1 body weight, (daily by oral route), and (P + A) co-treatment with piperine and AFB1. The experiment was conducted for 26 days. Chicks intoxicated with AFB1 showed significant genotoxic effects in the first 24 h post intoxication, and the effects remained in the other periods analyzed (48, 72, and 96 h and 26 days of treatment). The DNA damage in peripheral blood cells, the number of erythrocytes with micronuclei, and polychromatic-to-normochromatic erythrocyte ratio were significantly reduced or absent in the piperine/AFB1 group. No significant differences were observed between the group piperine/AFB1 and the control and piperine-alone groups. The addition 60 mg·kg−1 of piperine to the diet of the broiler chicks was safe, promoting beneficial effects in poultry health with respect to the toxic effects 0.5 mg·AFB1·kg−1 body weight.

Herbal Bioenhancers in Veterinary Phytomedicine

“…For more, piperine decreases the cytotoxicity and genotoxicity of aflatoxin B1, through inhibition of metabolization pathways shown in vitro ( 39 ). In broiler chickens intoxicated by AFB1 (ingestion of 0.5 mg AFB1 kg −1 bw); piperine (60 mg kg −1 ) was able to reduce or even prevent the genotoxic and cytotoxic effects, presenting a safe option for the protection and supportive treatment of aflatoxicosis ( 40 ).…”

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

“…c c e p t e d M a n u s c r i p tThe beneficial potential of certain chemicals (β-glucans, equol, vitamin E) and specially developed feed additives that protect animal health as well as deficiency of certain micronutrients and effects of feed restrictions was also assessed. Results showed protective properties against DNA damaging effects of mycotoxins and hydrogen peroxide or the type of diet used during the production indicating a genoprotective effects, in addition to the DNA damaging effects of certain stressors[291][292][293]297,299,[301][302][303][304].…”

Effects of gamma-aminobutyric acid and piperine on gene regulation in pig kidney epithelial cell lines

“…Moreover, PIP has been proposed as an alternative growth promoter to antibiotics in animal diets [9,25]. However, while its beneficiary effects in poultry has been investigated [25][26][27][28], its impact on porcine health remains relatively elusive. In previous study, we have previously demonstrated that expression of EPO and EPO-R is upregulated by GABA and PIP in combination in epithelial kidney cell lines through MAPK pathway [11].…”