Perfil proteico de tilápia nilótica chitralada (Oreochromis niloticus), submetida ao estresse crônico por hipóxia

Abstract

Perfil proteico de tilápia nilótica chitralada (Oreochromis niloticus) RESUMOAvaliou-se a variação da resposta secundária ao estresse causado por hipóxia durante 18 dias, em sistema de recirculação, em uma linhagem de tilápia nilótica (Oreochromis niloticus), chitralada, refletida no perfil proteico eletroforético do peixe e avaliou-se a diferença entre sexos para essa resposta. Foram utilizados 126 peixes, sendo 60 machos e 66 fêmeas, ambos com média de peso de 800g. O estresse crônico por hipóxia alterou (P<0,05) os valores médios relativos de albumina, α+β-globulinas e de γ-globulina, provocou alteração (P<0,05) nos níveis de proteína total relativo ao aumento no grupo dos machos, diminuição significativa dos valores médios absolutos de albumina devido à diminuição nas fêmeas e à diminuição de γ-globulina nos machos. As variantes proteicas, albumina e γ-globulina tiveram influência do sexo.

Physiological responses of pacu (Piaractus mesopotamicus) treated with homeopathic product and submitted to transport stress

“…The total protein is altered mainly by changes in the plasma volume, where an increase is caused by a change in the plasma fluid to the intracell compartment and decrease can be caused by plasma hydration. 23,24 The slight decrease in this parameter observed in this study after transport may be explained by the influx of water by osmosis that occurs in freshwater fish resulting from the increase in the branchial epithelium permeability by the rise in circulating catecholamines in response to transport stress. A similar result was reported by Melo et al 24 who observed decrease in the total plasma protein concentration in female Nile tilapia (O. niloticus) after hypoxia.…”

“…23,24 The slight decrease in this parameter observed in this study after transport may be explained by the influx of water by osmosis that occurs in freshwater fish resulting from the increase in the branchial epithelium permeability by the rise in circulating catecholamines in response to transport stress. A similar result was reported by Melo et al 24 who observed decrease in the total plasma protein concentration in female Nile tilapia (O. niloticus) after hypoxia. In matrinxã (B. amazonicus), Abreu and Urbinati 25 did not report significant differences for plasma protein after submitting the fish to stress by capture and air exposure.…”

Hematological Parameters of the Hybrid Serrasalmids Farmed in Central-Western Brazil

“…In tambacu, higher values were observed in the warm season. Changes in TPP are mainly caused by alterations in plasmatic volume due to osmotic disequilibrium between intracellular and extracellular compartments, so any stress that induces this situation may alter the total protein values (Melo et al, 2009). A decrease in TPP may also occur as a consequence of alimentary restriction (Chagas et al, 2005), which could have been the cause of the lower values observed in the cold season, given that at lower temperatures fish reduce their feeding.…”

Hematoimmunological responses of Nile tilapia juvenile with dietary supplementation of immunomodulators and different levels of vitamins after acute and physical stress

“…Higher levels of Ig, mainly with the inclusion of immunomodulators, may be related to an immune system response to the stresses suffered by these animals, because according to Plasma total protein helps determine the overall condition of the sh. This parameter is also very sensitive to stress (Melo et al 2009). In a simpli ed way, PTP is obtained by calculating the sum of albumins and globulins present in the plasma (Rodrigues et al 2018).…”

“…al. 2015, for cold-stressed Nile tilapia, in which the inclusion of 0.8% β-glucans and 600mg kg-1 of vitamin C obtained the lowest concentration of total protein and the highest concentration of globulins, characterizing a response to the stressor.Other studies(Abdelhamid et al 2020) con rmed that β-glucans tended to decrease the stress caused by diazinon (pesticide) in Nile tilapia Melo et al 2009. observed that males of Nile tilapia subjected to hypoxia had higher concentrations of total protein and females had higher concentrations of albumin and γ-globulin.…”