Efeito da prednisona em lesão medular aguda experimental em ratos

Silva, C.M.O.; Melo, Eliane Gonçalves de; Almeida, Andrés; Gomes, MF; Silva, C.H.O.; Rachid, Milene Alvarenga; Junior, Duarte; Vieira, N.T.; Franca, Suelene Aires

doi:10.1590/s0102-09352008000300018

Cited by 5 publications

(9 citation statements)

References 15 publications

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“…A difference (p=0.0008) was detected among groups for the locomotion capacity. The animals maintained at rest showed less locomotion capacity (10.23±2.12) Attal et al 1990) 0 Absent (distal region of limb -paw -the operated paw is pressed normally on the floor) 1 Discrete (distal region of limb -paw -the paw rests lightly on the floor and the toes are in a ventroflexed position) 2 Discrete to moderate (only the internal edge of the paw is pressed on the floor) 3 Moderate (only the talocalcaneal region -heel -of the paw is pressed on floor; hind paw is in an inverted position) 4 Moderate to intense (the whole distal region -paw -is elevated) 5 Intense (the animal licks the operated paws) Locomotion capacity (according to Silva 2008) 0 Absence of hindlimb movement; non-weight-bearing 1 Subtle movement, disconnected from the hindlimb; non-weight--bearing 2 Visible movement of hindlimb; non-weight-bearing 3 Supports load in hindlimb, but walks with accentuated deficit 4 Walk with moderate difficulty 5 Walk with a discrete deficit, and cannot walk on 7.7 cm wide bar 6 Normal walk or discrete deficit, and can walk on 7.7 cm wide bar 7 Can walk on 6.7 cm wide bar 8 Can walk on 5.7 cm wide bar 9 Can walk on 4.7 cm wide bar 10 Can walk on 3.7 cm wide bar 11 Can walk on 2.7 cm wide bar 12 Can walk on 1.7 cm wide bar than those of the other groups, with mean values and standard deviations of 10.73±1.22 (L), 11.25±0.99 (LE) and 11.37±0.92 (E). In the physical evaluation performed on day 21, only one animal from each group was able to move on the 1.7cm bar.…”

Section: Resultsmentioning

confidence: 99%

“…According to Silva (2008), the scale allows for identifying minimum changes that are often imperceptible during deambulation assessment in the open field. The authors used the scale to evaluate the motor capacity of Wistar rats subjected to an experimental spinal cord injury by using 50.5g stereotaxic equipment to apply pressure on the duramater for five minutes.…”

Section: Discussionmentioning

confidence: 99%

“…Locomotor capacity was assessed using scores that ranged from 0 to 12, according to the protocol described by Silva (2008), in which the higher the score the closer the animal was to normality. The evaluation was conducted through the observation of the movement of hindlimbs and gait; in other words, the deambulation of the animals in spontaneous locomotion.…”

Section: Methodsmentioning

confidence: 99%

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Histomorphometric analysis of the Achilles tendon of Wistar rats treated with laser therapy and eccentric exercise

Souza

Moreira²,

Silva³

et al. 2015

Pesq. Vet. Bras.

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Low-level laser therapy is recommended for the treatment of tendinopathies despite the contradictory results related to the ideal dose of energy, wavelength and time of application. This study aimed to assess the effects of laser therapy and eccentric exercise on tendinopathy of the Achilles tendon of Wistar rats. Forty-eight adult male rats were randomly distributed into four groups (L= laser; E= eccentric exercise; LE = laser and eccentric exercise; and R= rest). Laser therapy (904nm/3J/cm²) and/or eccentric exercise (downhill walking; 15 o incline treadmill; 12m/min; 50min/day) was started 24h after induction of unilateral tendinopathy and remained for 20 days. At 3, 7, 14 and 21 days after lesion induction, three rats from each group were euthanized and the tendons were collected for histological and morphometric analyses. There was no difference among groups or among times for the characteristics hemorrhage (p=0.4154), fibrinous adhesion formation (p=0.0712), and organization of collagen fibers (p=0.2583) and of the connective tissue (p=0.1046). For these groups, regardless of the time, eccentric exercise led to epitenon thickening (p=0.0204), which was lower in the group treated with laser therapy. Histological analysis revealed differences (p=0.0032) in the number of inflammatory cells over time. They were more numerous in the group that only exercised. This result was confirmed by morphometric analysis, which showed a significant interaction (groups x time) for this characteristic. Eccentric exercise increased (p=0.0014) the inflammatory infiltrate over time (3 and 21 days). However, association with laser therapy reduced inflammatory reaction. On the other hand, the combination of the treatments increased angiogenesis in morphometric (p=0.0000) and histological (p=0.0006) analyses compared with the other groups, while the isolated application of low-level laser reduced this characteristic over time. Animals maintained at rest presented the lowest amount (p=0.0000) of fibroblasts, according to the morphometric analysis. However, histological evaluation showed a significant group x time interaction (p=0.0024). Greater amounts of fibroblasts were observed in groups E, L and LE on the 7 th , 14 th and 21 st days, respectively. The animals that received laser therapy and were exercised showed a greater (p=0.0000) amount of collagen fibers over time. LE=laser e exercício excêntrico, e R=repouso). Após 24h da indução de tendinopatia unilateral, foi iniciada laserterapia (904nm/3J/cm²) e/ou exercício excêntrico (caminhada em declive; esteira com 15 o de inclinação; 12m/min; 50min/ dia) que permaneceu por 20 dias. Aos 3, 7, 14 e 21 dias após indução da lesão, três ratos de cada grupo foram submetidos à eutanásia, e os tendões foram obtidos para análi-ses histológica e morfométrica. Não houve diferença entre grupos nem entre tempos nas características hemorragia (p=0,4154), formação de aderência fibrinosa (p=0,0712) e organização das fibras colágenas (p=0,2583) e do tecido conjuntivo (p=0,1046). Considerando os g...

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Histomorphometric analysis of the Achilles tendon of Wistar rats treated with laser therapy and eccentric exercise

Souza

Moreira²,

Silva³

et al. 2015

Pesq. Vet. Bras.

View full text Add to dashboard Cite

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“…Após a realização da tricotomia e em plano anestésico, os ratos foram posicionados em decúbito ventral e feita antissepsia da pele com álcool-iodo-álcool.O acesso à medula espinhal foi realizado pela técnica de laminectomia dorsal em L3, conforme técnica descrita por SILVA et al (2008). Em seguida, nos ratos dos grupos GCS, GCM e GCP, foi realizada a síntese da musculatura epaxial, com padrão de sutura simples contínuo e da pele com Wolff, ambos com fi o de mononailon 4-0.…”

Section: Methodsunclassified

Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal

et al. 2015

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“…Os testes comportamentais foram realizados para avaliar a função neurológica dos animais, e basearam-se no método de escala descritiva adaptada (MEDA) já descrito anteriormente (Silva et al 2008, Torres et al 2010b) e consistiu na observação da capacidade motora (CM) e dos posicionamentos proprioceptivo (PP) e tátil (PT), bem como da permanência em estação no plano inclinado (PI), e da avaliação da sensibilidade dolorosa (SD …”

Section: Quadro 1 Grupos Experimentaisunclassified

Dantrolene e células-tronco mesenquimais promovem melhora funcional em ratos Wistar com trauma espinhal agudo

et al. 2018

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RESUMO.-Objetivou-se avaliar o efeito do dantrolene (DAN) e das células-tronco mesenquimais (CTM) no trauma espinhal agudo (TEA). Sessenta ratos Wistar foram divididos nos grupos CTM, DAN + CTM, DAN, trauma e placebo (TP) e sem trauma e placebo (STP). Realizou-se laminectomia de T12 em todos os grupos, seguida de TEA contusivo ∕ compressivo, com exceção do grupo STP. This study aimed to evaluate the effects of dantrolene (DAN) and mesenchymal stem cells (MSCs) in acute spinal cord injury (SCI). Sixty Wistar rats were divided into groups MSCs, MSCs + DAN, DAN, trauma and placebo (TP) and no trauma and placebo (STP). Laminectomy was performed at T12 level in all animals, followed by a weight-drop model of SCI, except for the STP group. An hour later, the MSCs + DAN and DAN groups received 10mg/kg of DAN. After seven days, the MSCs and MSCs + DAN groups received 1x10 6 cells intravenously. Behavioral tests were performed to assess functional recovery for 28 days. Traumatized animals showed paraplegia. There was a significant improvement in groups MSCs, DAN and MSCs + DAN compared to TP (p<0.05). It was concluded that DAN and MSCs for the treatment of SCI in rats have neuroprotection effect and promote functional neurological improvement.

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Efeito da prednisona em lesão medular aguda experimental em ratos

Cited by 5 publications

References 15 publications

Histomorphometric analysis of the Achilles tendon of Wistar rats treated with laser therapy and eccentric exercise

Histomorphometric analysis of the Achilles tendon of Wistar rats treated with laser therapy and eccentric exercise

Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal

Dantrolene e células-tronco mesenquimais promovem melhora funcional em ratos Wistar com trauma espinhal agudo

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