Peracute bovine mastitis caused by Klebsiella pneumoniae

Ribeiro, Márcio Garcia; Motta, Rodrigo Garcia; Paes, Antônio Carlos; Allendorf, Susan Dora; Salerno, Tatiana; Siqueira, Amanda Keller; Fernandes, Marta Catarina; Lara, Gustavo Henrique Batista

doi:10.1590/s0102-09352008000200031

Cited by 15 publications

(11 citation statements)

References 6 publications

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“…The study done in a separate geographical location in Sri Lanka showed that high prevalence of Staphylococcus spp, E.coli and Streptococcus spp [29]. In many studies, Staphyloccocus spp, E.coli and Klebsiella spp has been found as the common etiologies in line with the current study [30,31,32,33,34].…”

Section: Antibiotic Sensitivity Testsupporting

confidence: 85%

Isolation of Escherichia coli, Klebsiella spp. and Staphylococcus spp. from Bovine Mastitic Milk in Nuwera Eliya District of Sri Lanka and their Sensitivity to Commonly Used Antibiotics

Jayaweera

Randika

Gamage³

et al. 2018

SAJRM

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Aims: Mastitis is one of the very important and most common diseases among dairy cattle globally which leads to severe economical losses in the dairy industry. For the sustainability of the dairy sector it is critical that efficient, economically feasible treatment regime is available for clinical cases of mastitis as a part of the control program with minimum risk for residues in milk. Antimicrobials are the most common drugs of choice for controlling and preventing this devastating condition. But the frequent use of antibiotics leads to the development of resistant bacteria which could have an adverse effect on human health as well. To mitigate this destructive constraint in the industry, identifying the etiology and their susceptibilities to remedial measures are of paramount importance. Hence this study was aimed at isolating and identifying the common bacterial etiology Escherichia coli, Klebsiella spp. and Staphylococcus spp. of mastitis and evaluating the antimicrobial susceptibility of the isolates in order to develop mastitis control strategies in the area. Study Design: Milk samples were collected from mastitic cows in different stages including subclinical and clinical cases based on the results of California Mastitis Test Place and Duration of Study: Samples were collected from dairy farms in Nuwera Eliya District, Sri Lanka and Laboratory investigations were carried out in the Laboratory of Livestock Production, Faculty of Agricultural Sciences, Sabaragamuwa University of Sri Lanka, Between Aug. 2017 and Nov. 2017. Methodology: E.coli, Klebsiella spp. and Staphylococcus spp. were isolated from 31 milk samples and susceptibility to commonly used antibiotics (Trimethoprim, Oxytetracycline, Chloramphenicol, Cephalexin, Enrofloxacin and Ciprofloxacin) was determined by Kirby Bauer disk diffusion method. Results: The study revealed that the most common isolate was the Klebsiella spp. and it is 54.8% and other two organisms Staphylococcus spp. had 51.6% and Escherichia coli 41.9%. Of all isolated pathogen, 97.1% exhibited resistant to Cephalexin and it was the highest while lowest resistance was to Chloramphenicol (31.4%). Among the other antibiotics, 54.3% of total isolates showed resistance to Trimethoprim followed by 42.9% to Oxytetracycline and Enrofloxacin, 34.3% was resistant to Ciprofloxacin. Resistance to at least one antibiotic was observed for the isolated microorganisms. All the three isolated pathogens are more resistant to Cephalexin. Both E. coli and Klebsiella spp. show 100% resistance to Cephalexin while Staphylococci had 92.9% resistance. This further revealed that E. coli (10%) and Klebsiella spp. (27.3%) are showing the least resistance to Chloramphenicol, None of Staphylococcus spp. (0%) isolated show resistance to Enrofloxacin. Conclusion: Most common organisms isolated were Klebsiella spp. followed by Staphylococcus spp., E. coli and there is a resistance of isolated organisms to some commonly used antibiotics.

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Section: Antibiotic Sensitivity Testsupporting

confidence: 85%

Isolation of Escherichia coli, Klebsiella spp. and Staphylococcus spp. from Bovine Mastitic Milk in Nuwera Eliya District of Sri Lanka and their Sensitivity to Commonly Used Antibiotics

Jayaweera

Randika

Gamage³

et al. 2018

SAJRM

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“…Gram-negative bacteria, Escherichia coli and Klebsiella species in particular, are a common cause of clinical mastitis in dairy cattle (Erskine et al, 1991;Roberson et al, 2004). For many years, Klebsiella has received less attention than E. coli although Klebsiella species have been reported to threaten udder health in dairy herds as well, either by causing individual clinical mastitis cases (Todhunter et al, 1991;Ribeiro et al, 2008) or by causing mastitis outbreaks (Vecht et al, 1987;Sampimon et al, 2006;. Production losses and mortality due to IMI caused by Klebsiella are higher compared with those caused by E. coli (Erskine et al, 2002a;Gröhn et al, 2004), and current preventive vaccination and antibiotic therapy have only limited effects (Erskine et al, 2002b;Roberson et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Sources other than unused sawdust can introduce Klebsiella pneumoniae into dairy herds

Verbist¹,

Piessens²,

Nuffel³

et al. 2011

Journal of Dairy Science

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A longitudinal study was carried out to detect intramammary infections caused by Klebsiella pneumoniae and to identify potential sources of this bacterial species in the environment of the cows. The study was performed in 6 well-managed Belgian dairy herds from May 2008 to May 2009. Monthly (n=13), unused and used sawdust bedding samples as well as individual quarter milk and feces samples were collected from 10 randomly selected cohort cows in each herd. Cases of clinical mastitis of all lactating cows in the 6 herds were also sampled (n=64). From the 3,518 collected samples, 153 K. pneumoniae isolates were obtained, of which 2 originated from milk (clinical mastitis cases). In feces (n=728), used bedding (n=73), and unused bedding (n=73), respectively, 125 (17.2%), 20 (27.4%), and 6 (8.2%) isolates were found. The isolates were fingerprinted by means of pulsed field gel electrophoresis. In total, 109 different pulsotypes were differentiated, indicating a high degree of genetic diversity within the isolates. All isolates from unused bedding belonged to pulsotypes other than those from the other sources, suggesting that sources other than unused sawdust may introduce K. pneumoniae into the herd. Only 2 pulsotypes contained isolates originating from different sources. Pulsotype 10 was found in milk and used bedding and pulsotype 21 was found in feces and used bedding. The 2 milk isolates originated from 2 cows in the same herd but they belonged to a different pulsotype. The results indicate that K. pneumoniae can be prevalent in the environment without causing significant mastitis problems. Most cows were shedding K. pneumoniae in feces, substantiating findings under very different conditions (i.e., American dairy herds). Contamination of used bedding in the cubicles with K. pneumoniae from feces was confirmed, whereas unused bedding was not an important source of K. pneumoniae for the environment of the cows.

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“…K. pneumoniae was considered to be one of the most important zoonotic bacterial pathogens, and induced serious infection mainly due to hypervirulent K. pneumoniae (hvKp) strains (Jun 2018;Effah et al 2020). The hvKp infections have been associated with severe suppurative pneumonia in sheep and sea lion (Gameel et al 1991;Jang et al 2010), and widely reported as the causative agent of mastitis in cattle with a poor prognosis (Ribeiro et al 2008). Pulmonary infections in cattle caused by K. pneumoniae infection are very limited, primarily in Indonesian local cattle.…”

Section: Introductionmentioning

confidence: 99%

Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae

et al. 2020

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Abstract. Darniati, Setiyaningsih S, Agungpriyono DR, Handharyani E. 2021. Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae. Biodiversitas 22: 99-105. The aim of the research was to produce monospecific antibodies against Klebsiella pneumoniae serotype K1 and K2. Klebsiella pneumonia isolates were recovered from pneumonic lungs of Aceh cattle. The bacteria were identified by rpoB (RNA polymerase β subunit) PCR amplification specific for K. pneumoniae. The presence of capsule cluster gene magA and k2A was used to characterize capsular serotype K1 and K2, respectively. Antisera were produced using serial immunization of New Zealand White rabbits with K. pneumoniae serotypes K1 and K2. The presence of antibodies was determined by using immunodiffusion test and purification was performed by immunoaffinity purification method. K. pneumoniae antibodies began to appear on the 14th day after the first injection and progressively intensified after the 1st and 2nd booster. The cross-reactivity between the antigen was eliminated by absorbing the antisera with the opposite antigen and several serotypes of non-K1/K2. After purification, serum protein concentrations were substantially decreased from 58.48 µg/µL and 53.99 µg/µL to 2.38 µg / µL and 3.72 µg / µL for K1 and K2, respectively). The SDS-PAGE analysis showed two bands with molecular weight at 54 kDa and 25 kDa representing the heavy and light chains of immunoglobulin G, respectively. Purified polyclonal antiseras against K. pneumoniae serotypes K1 and K2 showed high affinity and specificity for homologous antigens in immunodiffusion and direct agglutination tests, and immunohistochemical staining.

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Peracute bovine mastitis caused by Klebsiella pneumoniae

Cited by 15 publications

References 6 publications

Isolation of Escherichia coli, Klebsiella spp. and Staphylococcus spp. from Bovine Mastitic Milk in Nuwera Eliya District of Sri Lanka and their Sensitivity to Commonly Used Antibiotics

Isolation of Escherichia coli, Klebsiella spp. and Staphylococcus spp. from Bovine Mastitic Milk in Nuwera Eliya District of Sri Lanka and their Sensitivity to Commonly Used Antibiotics

Sources other than unused sawdust can introduce Klebsiella pneumoniae into dairy herds

Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae

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