Parâmetros da degradação protéica ruminal de diferentes alimentos e rações estimados por técnica in vitro

Lana, R.P.; Leopoldino, Webel Machado; Oliveira, Juliana; Veloso, Rafael Gonçalves; Nunes, Poliana Mary Magalhães; Queiróz, Augusto César de

doi:10.1590/s0102-09352007000200023

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…According to Ball et al (1998), digestible crude protein ratio can decrease, especially if hay heats up due to excessive moisture. Morais et al (2004) registered digestibility of 700 g/kg CP in dwarf elephant-grass, while Lana et al (2007) studied the in vitro CP degradability of foods and found degradability values of 522 and 806 g/kg for Tifton 85 hay, with and without ammonization, respectively.…”

Section: Resultsmentioning

confidence: 99%

Use of a conditioning unit at the haymaking of Tifton 85 overseeded with Avena sativa or Lolium multiflorum

et al. 2012

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-White oat and ryegrass were overseeded on Tifton 85 pastures for evaluation of dry matter yield (DMY), leaf:stem ratio (L:S), dehydration curves and nutritional value of forages. Dry matter intake and L:S ratio were analyzed using a completely randomized blocks design with five forage growth conditions (single Tifton 85 and intercropped with white oat or ryegrass, and white oat or ryegrass intercropped with Tifton 85), whereas for drying curves, ten dehydration times were tested (0, 4, 8, 24, 28, 32, 48, 52, 56 and 71 hours after harvest) by a 5 × 10 split-plot arrangement (growth conditions of forages as main plot and dehydration times as sub-plots). The nutritive value data were analyzed in a 3 × 3 split-plot trial with single Tifton 85, Tifton 85 -Oat and Tifton 85-Ryegrass as main plots and the three sampling times (before harvesting, at baling and 100 days after storage) as sub-plots. Oat and ryegrass showed higher L:S ratio, while the intercrops of Tifton 85 -Oat and Tifton 85 -Ryegrass presented lower DMY, but higher crude protein concentration and in vitro DM digestibility than single Tifton. Neutral and acid detergent fiber concentrations were higher for single Tifton 85. Ryegrass and white oat, overseeded on Tifton 85 pasture, improved the nutritional value of the hay produced; however, in a 100-day storage period, there was a reduction in in vitro dry matter digestibility and crude protein concentrations.

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Section: Resultsmentioning

confidence: 99%

Use of a conditioning unit at the haymaking of Tifton 85 overseeded with Avena sativa or Lolium multiflorum

et al. 2012

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“…During the fermentation process there is a reduction of protein nitrogen and a considerable increase in NH 3 -N caused by proteolysis and deamination of proteins and amino acids. The insoluble but potentially degradable protein of feeds is converted to ammonia (Danley & Vetter, 1973;Lana et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Ammonia levels on <i>in vitro</i> degradation of fibrous carbohydrates from buffel grass

Neto¹,

Oliveira²,

Oliveira³

et al. 2019

SA J. An. Sci.

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This study was carried out to examine the degradation dynamics of neutral detergent fibre (NDF) and the profile of volatile fatty acids that originate from the fermentation of buffel grass (Cenchrus ciliaris, L.) with various levels of ammonia in the growth medium. The treatments consisted of six levels of ammonia in the growth medium (0, 5, 10, 15, 20 and 30 mg/dL), which were obtained by adding urea. These in vitro incubation times were evaluated in three replicates per time for 0, 3, 6, 9, 12, 24, 36, 48, 72 and 96 hours. Rumen concentrations of acetate and propionate responded quadratically to the ammonia levels. The treatment with 15 mg/dL of ammonia nitrogen in the rumen fluid provided mean acetate and propionate contents of 57.6 mM and 23.1 mM, respectively. Urea addition elevated the degradation rate of potentially degradable fraction of NDF (pdNDF) from 2.5% to 20.1% in comparison with the treatment without urea and to a reduction in estimated discrete lag time from 0.34 to 2.31 hours. Urea supplementation increased the specific microorganism growth rate from 2.6% to 20.1%. At the end of the incubation trial, NDF degradation showed a quadratic response, with maximum value obtained at 17.76 mg/dL of ammonia in the rumen fluid. Urea improves the degradation dynamics of NDF from deferred buffel grass and increases the concentrations of acetate and propionate.

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Effect of whey fermented by Enterococcus faeciumin consortium with Veilonella parvulaon ruminal bacteria in vitro

et al. 2014

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-The objective of this research was to evaluate the effect of whey fermented by Enterococus faecium in consortium with Veilonella parvula in vitro on ruminal microorganisms in different substrates, with or without monensin. The first experiment was carried out in a completely randomized design, in a 6 × 3 factorial arrangement (six substrates × three whey levels) with two replicates. In experiment two, a 2 × 3 × 4 factorial arrangement (with and without monensin, three foods and four levels of fermented whey) was used, in a randomized design with four replicates, totaling 24 treatments. There was no interaction among the wheys and the substrates in the variable for pectin, starch, and carboxymethyl cellulose. There was a greater growth of amylolytic and pectinolytic microorganisms and a lower growth of proteolytic and cellulolytic microorganisms. A significant effect of optical density was found in the media without substrate and that containing trypticase and glucose due to the addition of fermented whey. There was interaction for the pH at 24 hours among whey, food and monensin. For ammonia at 24 hours there was effect for food, whey and monensin, and interaction among factors. For microbial protein at 24 hours, there was effect for food, whey, monensin and no interaction among sources of variation. The use of whey fermented by bacteria Enterococcus faecium and Veilonella parvula improves microbial protein synthesis by ruminal bacteria in media containing different energy sources. The combination of fermented whey and monensin shows variable results in relation to microbial growth.

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Parâmetros da degradação protéica ruminal de diferentes alimentos e rações estimados por técnica in vitro

Cited by 3 publications

References 14 publications

Use of a conditioning unit at the haymaking of Tifton 85 overseeded with Avena sativa or Lolium multiflorum

Use of a conditioning unit at the haymaking of Tifton 85 overseeded with Avena sativa or Lolium multiflorum

Ammonia levels on <i>in vitro</i> degradation of fibrous carbohydrates from buffel grass

Effect of whey fermented by Enterococcus faeciumin consortium with Veilonella parvulaon ruminal bacteria in vitro

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