Fontes de nitrogênio, polpa de banana e ágar no desenvolvimento in vitro de plântulas de orquídea

Pasqual, Moacir; Figueiredo, Madeleine Alves de; Rezende, Juliana Costa de; Araujo, Aparecida Gomes de; Santos, Flávia Carvalho; Ferreira, Ester Alice; Junqueira, Keize Pereira

doi:10.1590/s0102-05362009000200016

Cited by 8 publications

(7 citation statements)

References 5 publications

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“…The banana pulp supplement was unfavorable to shoot development given that it produced results that were lower than those achieved in the treatment without supplementation (Figure 2A). This result differs from that of Pasqual et al, (2009) who worked with Cattleya loddigesii and obtained positive in vitro growth responses using this supplement. Silva et al (2005) states that banana pulp may provide amino acids and vitamins that increase the fresh mass of orchid species.…”

Section: Resultscontrasting

confidence: 99%

“…However, these data contradict those obtained in a study on Stanhopea tigrina (MARTÍNEZ; GARCÍA, 2007) where the best shoot development occurred when the seedlings were cultivated in pure MS supplemented with higher concentrations of coconut water (120 mL L -1 ) and banana pulp (100 g L -1 ) than those used in the current study. Furthermore, a study on Cattleya loddigesii (PASQUAL et al, 2009) showed that 200 g L -1 of banana pulp promoted greater seedling vigor. The differences in these results may be due to the specifications of the culture medium for different species since different combinations of carbohydrates, minerals, vitamins and growth regulators stimulate (or not) the growth of organs, tissues, cells and plant development (George et al, 2008), as is clearly shown by the current study.…”

Section: Ms Salt Concentration (%)mentioning

confidence: 99%

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Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog.

et al. 2016

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Dipteryx alata Vog. (barueiro) is a native fruit of the Cerrado used in food and medicine. The species has never been domesticated and is currently at risk of extinction. Plant tissue culture is an important tool for the conservation of germplasm and for quick, large-scale propagation of quality seedlings. While, this technique has never been used with barueiro, it could be a valuable tool in the domestication of this species. The objective of this work is to evaluate concentrations of MS medium salts (25, 50, 75 and 100%) supplemented with coconut water (100 mL L -1 ), dwarf banana pulp (60 g L -1 ) or the absence of any supplement in the in vitro establishment of barueiro seeds (Dipteryx alata Vog). The experiment used a completely randomized design (CRD) with a 3x4 factorial scheme (three cultivation media types and four concentrations of MS salts) and three repetitions. The following characteristics were evaluated: germination rate, shoot length , largest root length , stem diameter , number of leaves , chlorophyll A , chlorophyll B , total chlorophyll, and the fresh and dry mass of the seedlings. The coconut water and banana pulp supplements did not improve the in vitro development of the barueiro seedlings and the full concentration of MS salts did not improve the initial development of the seedlings. However, the pure MS medium at a concentration of 25% MS salts was the best option for the in vitro establishment of Dipteyx alata.

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Section: Resultscontrasting

confidence: 99%

Section: Ms Salt Concentration (%)mentioning

confidence: 99%

Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog.

et al. 2016

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“…podem ser cultivadas tanto em meio MS quanto em meios alternativos, como o simplificado à base de fertilizante Biofert® e o MS acrescido de polpa de banana. Pascoal et al (2009) constataram que o uso de polpa de banana nanica melhorou o desempenho do meio Knudson C na multiplicação in vitro de Cattleya loddigesii Lindl., orquídea de apelo ornamental. Ferreira et al (2010) testaram a germinação e desenvolvimento in vitro de Baptistonia pubes (Lindl.)…”

Section: Discussionunclassified

“…Estes meios podem ser modificados, com a adição de polpa de frutas ou fertilizantes comerciais, visando melhor desenvolvimento e maior vigor de plântulas (Stewart & Kane 2006;Pascoal et al 2009). O uso de polpa de frutas dificulta a reprodutibilidade dos resultados obtidos devido à variação nutricional, todavia há o barateamento do processo de obtenção de plântulas em larga escala e a facilitação técnica para produtores pouco especializados (Thompson et al 2006;Ferreira et al 2010;Favetta et al 2014).…”

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Cultivo in vitro de Epidendrum nocturnum (Orchidaceae) ocorrente no Cerrado da região Centro-Oeste

et al. 2016

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ResumoAs orquídeas podem ser propagadas in vitro em meios de cultivo assépticos ou em simbiose com fungos micorrízicos. Epidendrum nocturnum ocorre em áreas de Cerrado e neste estudo objetivou-se a visualização de fungos micorrízicos em suas raízes bem como o cultivo assimbiótico de suas plântulas em diferentes meios e a micorrização in vitro. No desenvolvimento assimbiótico testou-se três meios de cultura em um ensaio e, em outro ensaio, foram testadas formulações alternativas do meio Knudson C modificado, com a adição de polpa de frutas. Fungos micorrízicos de orquídeas foram usados na micorrização in vitro. Pelotons foram visualizados nas raízes de E. nocturnum. O meio Knudson C modificado diferiu estatisticamente dos demais, nas duas intensidades luminosas, no primeiro ensaio. No segundo ensaio, após sete meses, os meios com formulação alternativa não diferiram estatisticamente entre si. Na micorrização in vitro as plântulas com o isolado fúngico En07 (Waitea circinata) apresentaram maior vigor visual em relação aos demais tratamentos, mas não houve diferença estatística com o controle. Os meios com formulação alternativa e o Knudson C modificado podem ser utilizados no desenvolvimento in vitro de E. nocturnum e o isolado En07 pode ser usado na micorrização visando estratégias de conservação desta orquídea. Palavras-chave: orquídea, desenvolvimento assimbiótico, micorrização, fungo. AbstractOrchids can be propagated in vitro in aseptic culture media or in symbiosis with mycorrhizal fungi. Epidendrum nocturnum occurs in areas of Cerrado and this study aimed to mycorrhizal fungi visualization in their roots and its asymbiotic growing their seedlings in different media and in vitro mycorrhization. In asymbiotic development was tested three culture in a test and, another test, alternative formulations of medium Knudson C modified with addition of fruit pulp. Mycorrhizal fungi orchids were used in in vitro mycorrhization. Pelotons were visualized in the roots of E. nocturnum. The medium Knudson C modified statistically different from the others, the two light intensities, in the first test. In the second test, after seven months, with the alternative formulation means were not statistically different from each other. In vitro mycorrhization plantlets with isolated fungal En07 (Waitea circinata) showed greater visual effect compared to other treatments, but there was no statistical difference with control. The media with alternative formulation and Knudson C modified can be used in vitro development of E. nocturnum, and the isolated fungal En07 can be used in order mycorrhization of this orchid conservation strategies.

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“…Supplementations and modifications of the classical culture media from Burgeff (1909), Knudson (1946) and Murashige and Skoog (1962), for example, have been evaluated to find out the nutritional needs of orchids and the addition of complex compounds like banana pulp, coconut water, silicon, banana and potato extract are used to increase the macro and/or micronutrients content, vitamins, amino acids or even growth regulators (Colombo et al, 2016;Herrmann;de Freitas;Périco, 2011;Pasqual et al, 2009;Vieira et al, 2009). Several types of research explored the applicability of commercial fertilizers, combined or not with organic additives (simplified or alternative medium), as a strategy for preservation of vulnerable and endangered species or to reduce costs of in vitro commercial orchids production, such as Catasetum fimbriatum and Cyrtopodium paranaensis (Rego-Oliveira;Faria, 2005); Cattleya loddigesii (Moraes et al 2009); Laeliocattleya schilleriana Rolfe (Cunha et al, 2011); Cattleya trianaei (Galdiano Junior;Mantovani;Macedo Lemos, 2012); Cattleya walkeriana Gardner (Rodrigues et al, 2012b); Phalaenopsis (Colombo;Favetta; and Cattleya intermedia (Freitas et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Commercial fertilizers and organic additives in orchid micropropagation

Moraes

Camolesi²,

Palmieri

et al. 2020

PCC&M

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In vitro cultivation strategies promise conservation and micropropagation of vulnerable or economically exciting plants, especially in Orchidaceae. Numerous reports are available for an extensive contingent of orchids of the most varied genera, and the main issue is related to the behavior of species during the process of germination and growth concerning the nutrients used in the culture media. In Orchidaceae, several evidence support that each species responds differently and specifically according to the biotic and abiotic conditions available for in vitro growth. The main concern is to seek simplified culture media that promote germination and the development of healthy plants on a large scale. This study assessed the behavior of Catasetum fimbriatum and Catasetum macrocarpum in culture media containing commercial fertilizers supplemented with organic additives, compared with traditional media, an economically more viable strategy for micropropagation. Seeds were cultured on traditional nutrient media or with commercial fertilizer and organic additives in different combination. The analysis of plant growth after 120 days in vitro showed that the best treatment in both species was the medium containing commercial foliar fertilizer Peters NPK ® 10:30:20 supplemented with potato and banana pulp.

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Fontes de nitrogênio, polpa de banana e ágar no desenvolvimento in vitro de plântulas de orquídea

Cited by 8 publications

References 5 publications

Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog.

Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog.

Cultivo in vitro de Epidendrum nocturnum (Orchidaceae) ocorrente no Cerrado da região Centro-Oeste

Commercial fertilizers and organic additives in orchid micropropagation

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