Divergência genética em tomate estimada por marcadores RAPD em comparação com descritores multicategóricos

Abstract

Genetic divergence among tomato accessions using RAPD markers and its comparison with multicategoric descriptorsThe estimation of genetic variability in a germplasm bank is important not only for the conservation of the genetic resources, but also for applications in plant breeding. The genetic divergence among 78 tomato accessions was studied, based on 74 RAPD markers. Also, a correlation between the molecular profile and 27 morphological and agronomic data was performed. Cluster analysis (UPGMA), used to study the data, resulted in 13 groups that were correlated with five descriptors (growth habit, leaf type, fruit color, locule number, and fruit shape). Some groups had particularities, such as group IV that assembled accessions with pear shape fruits; group VII, that clustered accessions with bacterial wilt resistance, and group IX, that gathered accessions with potato leaf type. Bootstrap analysis revealed few consistent clusters. The results showed a positive and significant correlation between the matrixes generated out of qualitative and molecular data (t = 14.02). Mantel's correlation was highly significant, but with a low value (r = 0.39), which suggests that for a wise use of the germplasm bank accessions, both characterization, molecular and morphoagronomic, should be carried out.

Morphological characterization and analysis of genetic variability among pepper accessions

“…In agreement with this study, other studies with different oleraceous plants also described not having correlation (GONÇALVES et al, 2008;RÊGO et al, 2011;MOULIN et al, 2012).…”

“…Many species of the Capsicum genus, are still poorly worked, from genetic improvement point of view (RODRIGUES et al, 2012). GONÇALVES et al (2008) decribed that the characterization can be morphological, phenotipical reproductive, biochemical, cytogenetic or molecular. In this context,studies with morphological markers make significant contributions to the understanding of the genetic diversity.…”

Genetic divergence among processing tomato hybrids and formation of new segregating populations

“…However, as a consequence, there has been a low genetic gain obtained with intrapopulational breeding strategies (Gonçalves et al, 2008;Figueiredo et al, 2016a), which have opted for strategies that seek to recombine existing genetic variability in order to form segregating populations of wide genetic variability and to encourage the selection of transgressive genotypes (Figueiredo et al, 2016b).…”

Comparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and restricted fragment length polymorphism among fenugreek accessions

“…Therefore, estimation of the genetic diversity among plants is important for the improvement of any crop and for preserving natural variation for adaptation (Mondini et al, 2009). Genetic diversity can be determined using morphological, biochemical, and molecular markers (Gonçalves et al, 2008). These markers differ from each other with respect to important features such as genomic abundance, level of polymorphism detected, locus specificity, reproducibility, technical requirements, cost, and the type of data that they generate.…”

“…Molecular markers, particularly DNA genetic markers, are valuable in that they show genetic differences on a more detailed level without interference from environmental influences (Kumar et al, 2009), and involve techniques that provide fast results detailing genetic variation and reflecting underlying genetic diversity (Gonçalves et al, 2008). Furthermore, DNA polymorphisms have become the markers of choice for investigating phylogenetic relationships among various plant varieties (Martosa et al, 2005) and for molecular characterization (Singh et al, 2010).…”