Organogênese de ápices meristemáticos de batata em meios de isolamento e multiplicação in vitro

Pereira, Jonny Everson Scherwinski; Fortes, Gérson Renan de Luces

doi:10.1590/s0102-05362004000200007

Cited by 7 publications

(5 citation statements)

References 5 publications

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“…According to the same authors, higher biomass production is obtained when the material is cultivated in liquid medium composed of MS salts at full concentration, supplemented with pantothenic acid (5.0 mg L -1 ), thiamine (1.0 mg L -1 ), gibberellic acid (0.25 mg L -1 ), and sucrose (20 g L -1 ) under constant shaking. Similar result was observed for the organogenesis of potato meristematic apices (PEREIRA; FORTES, 2004b), in which the liquid culture medium had a higher multiplication rate when compared with the semi-solid medium.…”

Section: Multiplicationsupporting

confidence: 81%

Application of tissue culture techniques in potato

Morais¹,

Asmar²,

Silva³

et al. 2018

Biosci. J.

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Potato is the world's most important non-cereal food crop, and therefore, it is considered one of the major food sources for humankind. Its conventional propagation is asexual, by using the tuber, which allows the accumulation and dissemination of pathogens to new cultivation areas. This fact not only impairs the yield of this solanaceous plant, but also threatens the maintenance of genotypes for commercial or breeding purposes. Due to the impossibility of using botanical seed, conservation and exchange of germplasm of this species by means of conventional methods are not feasible. In all potato-producing regions, the demand for high-quality tubers has been paramount to ensure crops production. Thus, biotechnological techniques based on tissue culture are very important. Plant tissue culture offers alternative methods of propagation by in vitro techniques that provide production and multiplication of material with high sanity. Thus, this literature review summarizes the history and current situation of tissue culture techniques applied to potato crop. Besides clonal multiplication, this biotechnological tool makes available initial indexed material to breeding programs and certified seed potato, and facilitates the exchange and conservation of germplasm. For all these reasons, the use of these techniques in potato production chain directly benefits producers by providing high-quality propagules.

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Section: Multiplicationsupporting

confidence: 81%

Application of tissue culture techniques in potato

Morais¹,

Asmar²,

Silva³

et al. 2018

Biosci. J.

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“…The number of buds, number of shoots, number of leaves, callus formation (%) and number of roots per explant were evaluated. The average multiplication rate was estimated by counting the number of buds formed, except the apical bud (Pereira & Fortes, 2004).…”

Section: Methodsmentioning

confidence: 99%

6-Benzylaminopurine and 3-Indolebutyric acid on the in vitro multiplication of Eugenia involucrata

2021

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Eugenia involucrata is an important Brazilian forest species, which it can be used for timber, such as firewood and charcoal, and non-timber purposes, for culinary and medicinal use. Considering the limitations of its seminal reproduction, vegetative propagation, particularly by micropropagation, becomes an alternative for plantlets production. In this study we evaluated the effect of the phytoregulators 6-benzylaminopurine (BAP) and 3-indolebutyric acid (IBA) on the in vitro multiplication stage of nodal segments. The experiment was carried out in a completely randomized design, in a 3 x 4 factorial array, the main factors were BAP (0; 0.1 or 0.2 µM) and IBA (0; 10; 20 or 30 µM). After 60 days of in vitro inoculation, evaluations on the the number of buds, number of shoots, number of leaves, callus formation (%) and number of roots per explant were carried out. BAP had not significant effect, however, the use of IBA alone favours the callogenesis and negatively affected leaves, shoots and roots formation. A moderate multiplication rate was obtained when considering in vitro bud formation. BAP and IBA are dispensable in in vitro multiplication of E. involucrata.

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“…However, data found in the literature differ as to the appropriate consistency for potato. The use of liquid culture medium has provided equal or even greater efficiency for the multiplication rate of plant material (Pereira & Fortes, 2004). Therefore, it is necessary to investigate what is the best consistency of the culture medium for the development of in vitro potato plantlets.…”

Section: Introductionmentioning

confidence: 99%

Effect of agar concentration in culture medium on in vitro development of potato plants

Matos

Pereira

Balena

et al. 2020

RSD

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In vitro propagation and tissue culture technique is an alternative for obtaining healthy potato seeds. However, data found in the literature differ on the optimal consistency of the culture medium to be used in vitro. The aim in this research was to look at the adaptation of potato cultivars to medium with different agar concentrations. The experimental design was entirely randomized in a factorial scheme. The experiment tested the effect of 3 agar medium concentrations (solid, semisolid and liquid) on 6 cultivars (Agata, Bel, Camila, Catucha, Clara and Eliza), with 5 repetitions. We quantified from the plantlets, fresh and dry mass of roots and shoot, the number of nodes, the multiplication capacity and vigor. The study showed that potato cultivars respond significantly to different concentrations of agar and their best response was in the semisolid medium.

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Organogênese de ápices meristemáticos de batata em meios de isolamento e multiplicação in vitro

Cited by 7 publications

References 5 publications

Application of tissue culture techniques in potato

Application of tissue culture techniques in potato

6-Benzylaminopurine and 3-Indolebutyric acid on the in vitro multiplication of Eugenia involucrata

Effect of agar concentration in culture medium on in vitro development of potato plants

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