Using Tomato Analyzer software to determine embryo size in x-rayed seeds

Filho, Júlio Marcos; Gomes, Francisco Guilhien; Bennett, Mark A.; Wells, Arthur A; Stieve, Susan M.

doi:10.1590/s0101-31222010000200018

Cited by 20 publications

(23 citation statements)

References 14 publications

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“…Plant flowering is not completely uniform, even in cultivars with a determinate growth habit (which isn't the case in cucumber), consequently generating uneven pollination, fertilization and maturation (Marcos-Filho et al, 2010). Recent research has revealed that the percentage of seeds with the maximum degree of embryonic development (full seeds) was associated to the state of fruit maturation, i.e., when seeds were extracted from fruits harvested 30 days after anthesis, only 35% of the seeds were totally full, whereas when harvested at 35 days after anthesis, the percentage of full seeds had increased to 75% (Nakada et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…The radiographed seeds were then placed in individual cells in a plastic tray (100 cells/ tray) for germination. The embryonic area was determined from the X-rayed seeds using the Tomato Analyzer software (Brewer et al, 2008), according to the procedures described by Marcos-Filho et al (2010). Three categories of embryonic area were defined based on the mean (M) and standard deviation (sd) of the values obtained for each lot: category I -seeds with an embryonic area lower than the mean minus the standard deviation (<M-sd); category II -seeds with an embryonic area in the interval between the mean minus the standard deviation and the mean plus the standard deviation (M±sd); category III -seeds with an embryonic area greater than the mean plus the standard deviation (>M+sd).…”

Section: Methodsmentioning

confidence: 99%

“…One of the possible morphological variations associated with seed performance is the embryonic area, which is relationship between degree of embryo development and space available in the internal seed cavity (Marcos-Filho et al, 2010). The study of this characteristic has been mainly possible as a consequence of improvement in image analysis techniques, specifically for x-rays, allowing for the establishment of relationships with seed physiological potential.…”

Section: Introductionmentioning

confidence: 99%

“…An alternative for eliminating the inconsistency and subjectivity of that evaluation has been proposed by Marcos-Filho et al (2010), using a semiautomated evaluation of the embryonic area of cotton and cucurbit seeds with a Tomato Analyzer program, developed primarily for evaluating the phenotypic characteristics of tomato fruits (Brewer et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Semi-automated assessment of the embryonic area of cucumber seeds and its relationship to germination and seedling length

2013

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-Automated evaluations are highly useful for the seed industry to provide reliable information about different parameters of seed quality and fast results and to reduce errors based on human visual analysis. The objective of this research was to identify variations in the degree of embryo development in commercial cucumber seed lots and their relationship with physiological potential. Seeds of Supremo and Safira cultivars (four lots each), with germination above 90%, were X-rayed, evaluated according to embryonic area (using the Tomato Analyzer software) and germinated (4 days at 25 °C); germination percentage and seedling length data were obtained. The average seed embryo area was 79.7 and 80.9% (Supremo and Safira, respectively), ranging from 4.4 and 1.0 percentage points among the lots of each cultivar. The semiautomated analysis using the Tomato Analyzer software identifies small variations in the embryonic development of cucumber seed lots; seeds with an embryonic area less than 78.1% were more likely to produce less developed seedlings.Index terms: Cucumis sativus, quality control, imaging analysis, x-rays.Avaliação semiautomática da área embrionária de sementes de pepino e sua relação com a germinação e crescimento de plântulas RESUMO -A automação das avaliações de diferentes atributos da qualidade de sementes é altamente desejável pela indústria de sementes, pois além da rápida obtenção de resultados, reduz erros humanos de interpretação decorrentes da análise baseada em estimativas visuais. Com o objetivo de identificar variações do grau de desenvolvimento embrionário em sementes de pepino de lotes comerciais, bem como sua possível relação com o potencial fisiológico, sementes das cultivares Supremo e Safira (quatro lotes cada), com germinação mínima de 90%, foram radiografadas, submetidas à avaliação da área embrionária (software Tomato Analyzer) e avaliadas quanto à germinação (4 dias a 25 °C) e comprimento de plântulas. A área embrionária média das sementes foi de 79,7 e 80,9% (cultivares Supremo e Safira, respectivamente), com variações de 4,4 e 1,0 pontos percentuais entre os lotes de cada cultivar, respectivamente. A análise semiautomatizada, realizada com o auxílio do software Tomato Analyzer, possibilita a identificação de variações estreitas no grau de desenvolvimento embrionário de lotes de sementes de pepino, uma vez que as sementes cuja área embrionária ocupa espaço inferior a 78,1% da cavidade interna são mais propensas a originarem plântulas menos desenvolvidas.Termos para indexação: Cucumis sativus, controle de qualidade, análise de imagens, raios-x.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Semi-automated assessment of the embryonic area of cucumber seeds and its relationship to germination and seedling length

2013

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“…Neste sentido, a captura e o processamento da imagem radiografada têm permitido o estabelecimento de relações entre integridade, morfologia e determinação da germinação das sementes (Marcos Filho et al, 2010).…”

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Utilização De Raios X Na Avaliação Da Morfologia Interna De Sementes De Sorgo

Javorski¹,

Cícero²

2017

RBMS

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RESUMO -A utilização de raios X em sementes é uma técnica eficiente na avaliação da morfologia interna de sementes de diversas espécies; trata-se de um método rápido, preciso e não destrutivo. Assim, a presente pesquisa teve o objetivo de avaliar a morfologia interna das sementes de sorgo, por meio de raios X, e relacioná-la com a germinação. Para tanto foram utilizadas sementes do híbrido 50A70, representado por dez lotes. As sementes radiografadas foram submetidas ao teste de germinação, de modo a relacionar a morfologia interna das sementes com as respectivas plântulas normais, anormais ou sementes mortas. Concluiu-se que o teste de raios X é eficiente na identificação de danos por deterioração de tecidos, danos mecânicos, danos por malformação e danos por insetos, possibilitando estabelecer a relação entre o dano observado e o prejuízo causado para a germinação. Palavras-chave:Sorghum bicolor, análise de imagens, germinação. X-RAY IN THE EVALUATION OF INTERNAL MORPHOLOGY OF SORGHUM SEEDSABSTRACT -The use of X-ray in seeds is an efficient technique for the evaluation of the internal seed morphology; it is considered a fast, accurate and non-destructive method. Thus, this study aimed to evaluate the internal morphology of sorghum seeds through X-ray. Therefore, seeds of hybrid 50A70 were used, represented by ten lots. The X-rayed seeds were submitted to germination test in order to associate the internal morphology of the seeds with normal or abnormal seedlings and dead seeds. The results allowed concluding that the X-ray test is effective in identifying damage tissue deterioration, mechanical damage, malformation damage and insect damage, enabling to establish a relationship between the observed damage and the negative effect on germination.

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Discrimination of Urochloa seed genotypes through image analysis: Morphological features

et al. 2021

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The tropical forage species seed market, including signalgrass (Urochloa), is expanding. There is demand for fast, automated methods that can be implemented for quality control of the seeds produced, with the aim of evaluating physical purity. In this respect, seed morphological features obtained by processing radiographs with the Tomato Analyzer software and of red-green-blue obtained and processed on the Groundeye device were used to test differentiation of materials of the Urochloa genus. Seeds of Urochloa brizantha (A.Rich.) R.D.Webster cvv. BRS Piatã, Marandu, and Xaraés MG-5; Urochloa ruziziensis (R.Germ. & C.M.Evrard) Crins cv. Ruziziensis; and Urochloa decumbens (Stapf) R.D.Webster cv. Basilisk were evaluated. Morphological features obtained by Tomato Analyzer allowed differentiation of Urochloaseeds at an accuracy level greater than 80% for all the materials evaluated. Seed area was one of the features that allowed this differentiation. The Groundeye system also showed high efficiency in distinguishing Urochloa seeds, except for Piatã and Xaraés, which exhibited morphological similarity, and lower accuracy levels in distinguishing their seeds (<50%). Additional studies should be conducted to evaluate Urochloa seed lots from other origins to validate the use of image analysis techniques for the purpose of obtaining morphological features and to assist in determining the physical purity of the materials. INTRODUCTIONCorrect identification of species and even cultivars in the seed analysis laboratory is a time-consuming operation (Chaugule & Mali, 2016) because of the similarity among seeds of the same genus, or even as a result of the absence of specific morphological descriptors of the materials under Abbreviations: LDA, linear discriminant analysis; RGB, red-green-blue; WMIH, width at mid-height.

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Using Tomato Analyzer software to determine embryo size in x-rayed seeds

Cited by 20 publications

References 14 publications

Semi-automated assessment of the embryonic area of cucumber seeds and its relationship to germination and seedling length

Semi-automated assessment of the embryonic area of cucumber seeds and its relationship to germination and seedling length

Utilização De Raios X Na Avaliação Da Morfologia Interna De Sementes De Sorgo

Discrimination of Urochloa seed genotypes through image analysis: Morphological features

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