Caracterização de cultivares de soja por descritores morfológicos e marcadores bioquímicos de proteínas e isoenzimas

Vieira, Elisa Serra Negra; Pinho, Édila Vilela de Resende Von; Carvalho, Maria das Graças; Silva, Priscila Alves da

doi:10.1590/s0101-31222009000100010

Cited by 16 publications

(19 citation statements)

References 6 publications

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“…This wide variation between cultivars and stages of development can be explained by the fact that the esterases are the most polymorphic isoenzyme group in plants (WEEDEN; WENDEL, 1990); it can therefore be thought of as efficient for distinguishing radish seeds in the early stages of development. Vieira et al (2009) also considered that esterase may help in the characterisation of soybean cultivars by making it possible to separate the cultivars into groups when extracted from dry seeds. Ferreira et al (2009), working with gladiolus (Gladiolus sp.…”

Section: Resultsmentioning

confidence: 99%

“…The detection of isoenzymes was the first method of genetic analysis that was developed (BYRNE; LITTLETON, 1988). Isoenzymes were considered efficient in differentiating individuals of the same species and cultivar, such as the soybean (VIEIRA et al, 2009) and red palm (MARTINS et al, 2007). However, this marker is influenced by the environment, the phenological stage and the presence of pathogens (ALFENAS et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterisation of radish cultivars

Cruz¹,

Nery²,

Pinho³

et al. 2014

Rev. Ciênc. Agron.

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-The radish has been gaining importance in the Brazilian market due to its use as a green manure and cover crop, and also due to its potential for the production of biodiesel, however there are only two registered cultivars, which are morphologically very similar. Determination of genetic purity is a prerequisite in marketing seeds as it ensures uniformity of the crop and is important in breeding programs. The aim of this work therefore was to evaluate the similarity and genotype patterns which permit differentiation of the commercial radish cultivars IPR 116 and CATI AL-1000. In order to do this, isoenzyme electrophoretic patterns were analysed in dry seeds, soaked seeds, seedling leaves and young leaves; the isoenzymes used being: superoxide dismutase, catalase, esterase, glutamate-oxalocetate, malate dehydrogenase and isocitrate lyase. Thirtyseven RAPD primers and 10 ISSR primers in leaves of the CATI AL-1000 and IPR-116 cultivars were analysed. Among the isoenzymes under study, the most polymorphic were glutamate oxalocetate, malate dehydrogenase, esterase and superoxide dismutase, with the superoxide dismutase system giving the best characterisation for all stages of development. The catalase isoenzyme system did not make it possible to differentiate between cultivars at any stage of development, and isocitrate lyase was not revealed by the protocol used. In analysis of the markers, 27 RAPD primers and eight ISSR primers showed polymorphism. The results indicate that it is possible to determine reliable descriptors based on isoenzymes at different stages of development of the radish and with the use of RAPD and ISSR primers. RESUMO -O nabo-forrageiro tem ganhado importância no mercado brasileiro como adubo verde, planta de cobertura e pelo potencial para produção de biodiesel, no entanto estão registradas apenas duas cultivares que são morfologicamente muito semelhantes. A determinação da pureza genética é um dos requisitos para comercialização de sementes, pois garante a uniformidade de plantio, e é importante para os programas de melhoramento. Por tanto, objetivou-se com este trabalho avaliar a similaridade e padrões genotípicos que permitam diferenciar as cultivares comerciais de nabo-forrageiro 'IPR 116' e 'CATI AL-1000'. Para isso, foi analisado o padrão eletroforético de isoenzimas em sementes secas, sementes embebidas, folhas de plântulas e folhas jovens. As isoenzimas usadas foram: superóxido dismutase, catalase, esterase, glutamato -oxilacetato, malato desidrogenase e isocitrato liase. Foram analisados 37 primers RAPD e 10 primers ISSR em folhas das cultivares 'CATI AL-1000' e 'IPR-116'. Entre as isoenzimas estudadas, as mais polimórficas foram glutamato -oxilacetato, malato desidrogenase, esterase e superóxido dismutase, tendo o sistema superóxido dismutase realizado a melhor caracterização para todos os estádios de desenvolvimento. O sistema isoenzimático catalase não permitiu distinguir as cultivares em nenhum estádio de desenvolvimento e a isocitrato liase não revelou com o protocolo u...

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterisation of radish cultivars

Cruz¹,

Nery²,

Pinho³

et al. 2014

Rev. Ciênc. Agron.

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“…Therefore, certification of genetic purity of cultivars becomes an indispensable tool for ensuring a product with the genetic characteristics developed by the breeder, as well as guaranteeing protection of intellectual property (Gratapaglia and Ferreira, 1996;Vieira et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…However, these markers present limitations when there is narrowing of the genetic base among the cultivars, which makes differentiation among them difficult (Lanza et al, 2000;Vieira et al, 2009). Enzyme and DNA molecular markers have properties that make them extremely useful in characterization of cultivars.…”

Section: Introductionmentioning

confidence: 99%

Identification of earl millet cultivars using both microsatellites and enzymatic markers

Neto¹,

Pinho²,

Carvalho³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The increasing number of protected and registered cultivars and problems involving seed commercialization make distinction and identification of cultivars imperative. Millet (Pennisetum glaucum), a crop species with protected cultivars in Brazil, has been the target of seed piracy. Thus, with the objective of identifying different lots with regard to origin, we characterized six cultivars of commercialized millet of proven origin by means of the electrophoretic patterns of the isoenzymes alcohol dehydrogenase, esterase and glutamate oxaloacetate transaminase and by microsatellite markers, using primers specific for millet. The six cultivars were separated with four microsatellite loci. Based on this characterization, certification of genetic purity was undertaken for public domain commercialized seed lots. The isoenzymatic markers were also tested for stability of the patterns. Esterase patterns were altered in seeds with different physiological quality and health conditions, but this alteration did not hinder identification of the cultivars. It was observed that most of the millet seed lots commercialized in Brazil as being in public domain belong to other cultivars.

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“…Apesar de apresentarem limitações por requererem tempo e espaço e muitos serem avaliados em plantas adultas, os marcadores morfológicos são tradicionalmente usados na caracterização de cultivares, para o registro e proteção de cultivares (VIEIRA et al, 2009). Por meio da caracterização morfológica é fornecida uma série de informações a respeito da variabilidade genética de cada acesso estudado (GUIMARÃES et al, 2007).…”

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Identificação de cultivares e certificação da pureza genética de gladíolo por meio de marcadores morfológicos

et al. 2011

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RESUMOA caracterização de cultivares de flores é fundamental nos processos de registro e proteção dessas e também na certificação da pureza genética. Nesse trabalho foram realizadas a caracterização e a certificação da pureza genética de cultivares de Gladiolus sp. por meio de marcadores morfológicos. Utilizaram-se onze cultivares de Gladiolus sp., e para a caracterização das cultivares, as parcelas foram constituídas de 25 plantas avaliando-se características dos cormos, folhas e flores. O delineamento experimental adotado foi de blocos casualizados, com quatro repetições Para a certificação da pureza genética, foram instaladas parcelas constituídas de 24 plantas e cormos contendo contaminação varietal em diferentes proporções. Foram utilizadas três cultivares para compor os tratamentos e as avaliações foram conduzidas por três avaliadores que desconheciam a porcentagem existente de contaminação varietal. Foram calculadas as porcentagens de acerto e de erro com base nos resultados obtidos nas avaliações. Foi calculado o desvio padrão da média dos resultados nas quatro repetições, obtidos pelos três avaliadores. Para verificar a significância dos desvios entre os resultados obtidos pelos avaliadores foi utilizado o teste de qui-quadrado ( 2 ). Por meio de características do cormo é possível diferenciar quatro cultivares das onze avaliadas. A característica da cor das flores é o descritor morfológico mais seguro para a diferenciação das cultivares de gladíolo. Dependendo das características das cultivares contaminantes e das cultivares em análises é possível utilizar descritores morfológicos para a certificação de pureza genética em cultivares de gladíolo.Termos para indexação: Gladiolus sp, flores, contaminação varietal. ABSTRACTThe characterization of flower cultivars is fundamental in the registration and protection processes and also in the genetic purity certification. In this work, both the characterization and the genetic purity certification of cultivars of Gladiolus sp by means of morphologic markers were made. Eleven cultivars of Gladiolus sp. were used and for the characterization of the cultivars, the plots were made up of 25 plants, evaluating the characteristics of corms, leaves and flowers. The used experimental design was the one of randomized blocks with four replicates. For the genetic purity certification, plots with 24 plants and corms containing varietal contaminations in different proportions were established. Three cultivars were used to make up the treatments, and the evaluations were conducted by three evaluators who were unaware of the existent percentage of varietal contamination. The percentage of mistakes was calculated based upon the obtained results of the evaluations. The standard deviation of the means of the results in the four replicates obtained by the three evaluators was calculated. In order to verify the significance of the deviations among the obtained results by the evaluators, Chi-square test ( 2 ) was used. By means of corm characteristics, it is possible to distinguish ...

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Caracterização de cultivares de soja por descritores morfológicos e marcadores bioquímicos de proteínas e isoenzimas

Cited by 16 publications

References 6 publications

Molecular characterisation of radish cultivars

Molecular characterisation of radish cultivars

Identification of earl millet cultivars using both microsatellites and enzymatic markers

Identificação de cultivares e certificação da pureza genética de gladíolo por meio de marcadores morfológicos

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