Determination of pectin methylesterase activity in commercial pectinases and study of the inactivation kinetics through two potentiometric procedures

Gonzalez, Samantha Lemke; Rosso, Neiva Deliberali

doi:10.1590/s0101-20612011000200020

Cited by 16 publications

(9 citation statements)

References 11 publications

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“…To our knowledge, this is the first report of such a characterization for a PME from Arabidopsis. PME3-His 6 activity was optimal at an alkaline pH (pH 7.5), which is similar to that reported for apple, banana, and green pepper PMEs (14, 59 -61) and contrasts with that reported for fungal PMEs (62,63). Interestingly, at an optimal pH, PME3-His 6 activity was dependent on the DM and DP values and methylesterification distribution within HG.…”

Section: Discussionsupporting

confidence: 76%

Tuning of Pectin Methylesterification

Sénéchal

L’enfant

Domon

et al. 2015

Journal of Biological Chemistry

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Background: PME and PMEI isoforms are co-expressed in Arabidopsis. Their biochemical interaction is yet to be characterized. Results:The processive activity of AtPME3 is regulated by AtPMEI7 in a pH-dependent manner in vitro. Conclusion: AtPMEI7 is a key component of the regulation of AtPME3 activity in planta. Significance: The tuning of AtPME3 activity by AtPMEI7 brings insights into the control of homogalacturonan methylesterification in plant cell walls.

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Section: Discussionsupporting

confidence: 76%

Tuning of Pectin Methylesterification

Sénéchal

L’enfant

Domon

et al. 2015

Journal of Biological Chemistry

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“…Enzymes required for the breakdown of pectin can be majorly classified into three categories as protopectinases (involved in breaking insoluble protopectin and results in soluble polymerized pectin), depolymerizing enzymes (required for breaking down α(1 → 4) glycosidic linkages of pectin) and esterases (required for the de-esterification and de-acetylation of pectin) (Gonzalez and Rosso 2011). Pectinolytic enzymes are widely observed among plants, bacterial and fungal species and most of the pectin methyl esterases can be divided based on their optimum pH; bacteria and plant PME exhibit an optimum pH range between 6 and 8 and PME secreted by fungi exhibit a pH 4-6 (Gonzalez and Rosso 2011;Jayani et al 2005). Pectin-degrading enzymes have attained high commercial importance since early 1930s in wine and fruit juice industries; pectinolytic enzymes secreted by Aspergillus species are highly used in industries (Alkorta et al 1998).…”

Section: Carbohydrate Esterases De-acetylating Pectinmentioning

confidence: 99%

“…Calcium and magnesium pectate forms the major constituent of the plant cell walls especially in middle lamella (Jayani et al 2005). The gelling property of pectin majorly employed in the food industries is directly dependent on its degree of esterification; pectins with higher degree of esterification gel around pH 3.0 in the presence of sugar, whereas pectins with low degree of esterification gel in the presence of calcium ions under wide pH ranges and with or without sugar Rao 1999, 2001;Gonzalez and Rosso 2011).…”

Section: Carbohydrate Esterases De-acetylating Pectinmentioning

confidence: 99%

“…Pectin methyl esterases or alkaline reagents were majorly used to demethoxylate large galacturonic chain for reducing the overall pectin methoxylation content (Gonzalez and Rosso 2011). Majorly, pectinolytic enzymes were highly applied in fruit juice and wine industries for the clarification of the fruit juices, modification of fruits and vegetables (Kohli et al 2015).…”

Section: Carbohydrate Esterases De-acetylating Pectinmentioning

confidence: 99%

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Structural and functional properties of pectin and lignin–carbohydrate complexes de-esterases: a review

Kameshwar

Qin

2018

Bioresour. Bioprocess.

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Biological conversion of plant biomass into commercially valuable products is one of the highly studied subjects in the last two decades. Studies were continuously being conducted to understand and develop efficient enzymes for the breakdown and conversion of plant cell-wall components into valuable commercial products. Naturally, plant cell-wall components are differentially esterified to protect from the invading microorganisms. However, during the process of evolution, microorganisms have developed special set of enzymes to de-esterify the plant cell-wall components. Among the carbohydrate-active enzymes (CAZy), carbohydrate esterases stand first during the process of enzymatic conversion of plant biomass, as these enzymes de-esterify the plant biomass and make it accessible for the hydrolytic enzymes such as cellulases, hemicellulases, ligninolytic and pectinases. In this article, we have extensively discussed about the structural and functional properties of pectin methyl esterases, feruloyl, cinnamoyl and glucuronoyl esterases which are required for the de-esterification of pectin and lignin-carbohydrate complexes. Pectin esterases are classified among CE8, CE12, CE13 and CE15 carbohydrate esterase class of CAZy database. Whereas, lignin-carbohydrate complex de-esterifying enzymes are classified among CE1 (feruloyl esterase) and CE15 (glucuronoyl esterase) classes. Understanding the structural and functional abilities of pectin and lignin-carbohydrate esterases will significantly aid in developing efficient class of de-esterases for reducing the recalcitrant nature of plant biomass. These efficient de-esterases will have various applications including pretreatment of plant biomass, food, beverage, pulp and paper, textile, pharmaceutical and biofuel industries. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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“…The degradation of insoluble protopectin is performed by protopectinases, resulting in highly polymerised soluble pectin. However, hydrolysis of the α (1 → 4) glycosidic linkage in pectic acid is catalysed by depolymerases (Whitaker et al 2003 ; Jayani et al 2005 ; Gonzalez and Rosso 2011 ). PME (EC 3.1.1.11) is responsible for pectin degradation by catalysing the demethoxylation of the homogalacturonan chain of pectin to release methanol and acidic pectin (Micheli 2001 ).…”

Section: Enzymes Secreted By Cladosporium Cladosporioides mentioning

confidence: 99%

Cladosporium cladosporioides from the perspectives of medical and biotechnological approaches

AlMatar

Makky

2015

3 Biotech

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Fungi are important natural product sources that have enormous potential for the production of novel compounds for use in pharmacology, agricultural applications and industry. Compared with other natural sources such as plants, fungi are highly diverse but understudied. However, research on Cladosporium cladosporioides revealed the existence of bioactive products such as p-methylbenzoic acid, ergosterol peroxide (EP) and calphostin C as well as enzymes including pectin methylesterase (PME), polygalacturonase (PG) and chlorpyrifos hydrolase. p-Methylbenzoic acid has ability to synthesise 1,5-benzodiazepine and its derivatives, polyethylene terephthalate and eicosapentaenoic acid. EP has anticancer, antiangiogenic, antibacterial, anti-oxidative and immunosuppressive properties. Calphostin C inhibits protein kinase C (PKC) by inactivating both PKC-epsilon and PKC-alpha. In addition, calphostin C stimulates apoptosis in WEHI-231 cells and vascular smooth muscle cells. Based on the stimulation of endoplasmic reticulum stress in some types of cancer, calphostin C has also been evaluated as a potential photodynamic therapeutic agent. Methylesterase (PME) and PG have garnered attention because of their usage in the food processing industry and significant physiological function in plants. Chlorpyrifos, a human, animal and plant toxin, can be degraded and eliminated by chlorpyrifos hydrolase.

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Determination of pectin methylesterase activity in commercial pectinases and study of the inactivation kinetics through two potentiometric procedures

Cited by 16 publications

References 11 publications

Tuning of Pectin Methylesterification

Tuning of Pectin Methylesterification

Structural and functional properties of pectin and lignin–carbohydrate complexes de-esterases: a review

Cladosporium cladosporioides from the perspectives of medical and biotechnological approaches

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