Tuning of Pectin Methylesterification

Sénéchal, Fabien; L’enfant, Mélanie; Domon, Jean-Marc; Rosiau, Emeline; Crepeau, Marie-Jeanne; Surcouf, Ogier; Esquivel‐Rodríguez, Juan; Marcelo, Paulo; Mareck, Alain; Guérineau, François; Kim, HyungRae; Mravec, Jozef; Bonnin, Estelle; Jamet, Élisabeth; Kihara, Daisuke; Lerouge, Patrice; Ralet, Marie-Christine; Pelloux, Jérôme; Rayon, Catherine

doi:10.1074/jbc.m115.639534

Cited by 56 publications

(14 citation statements)

References 67 publications

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“…SALK_092577C ( gaut10–3 ) is a null allele characterized in this study. Other alleles used in this study have been previously published as null/knock-out mutants: vps35b-1 (SALK_014345) (15); bdx-2 (SALK_142260) (16); camta2 (SALK_007027) (17); gapcp2.2 (SALK_008979) (18); atrh8 (SALK_016830) (19); smp2 (SALK_127730) (20); pme17–2 (SALK_059908) (21); SALK_063023 (22); nadp-me4 (SALK_064163) (23); rhip1–1 (SALK_091518) (24); SALK_111575 (25); cka2–1 (SALK_129331) (26); SALK_145341 (27); SALK_151595 (28); and impl2–3 (SAIL_35_A08) (29). For proteomics profiling, Col-0 seeds were surfaced sterilized using 50% bleach and 0.01% Triton X-100 for 10 min and then washed five times with sterile water.…”

Section: Methodsmentioning

confidence: 99%

Quantitative Early Auxin Root Proteomics Identifies GAUT10, a Galacturonosyltransferase, as a Novel Regulator of Root Meristem Maintenance

Walley

Shen

et al. 2019

Molecular & Cellular Proteomics

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Auxin induces rapid abundance changes in various signaling proteins, transcriptional regulators, and enzymes such as cell wall modification proteins in roots. Loss of function of 15 top responsive proteins results in altered root phenotypes, demonstrating the power of this approach for reverse genetics screens. Characterization of the auxin responsive protein galacturonosyltransferase 10 demonstrates that this enzyme positively regulates sugar-mediated root meristem maintenance. Novel targeted proteomics assays demonstrate that all six auxin receptors remain stable in response to hormone.

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Section: Methodsmentioning

confidence: 99%

Quantitative Early Auxin Root Proteomics Identifies GAUT10, a Galacturonosyltransferase, as a Novel Regulator of Root Meristem Maintenance

Walley

Shen

et al. 2019

Molecular & Cellular Proteomics

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“…Interaction studies performed in vitro at different pH values (pH 5.5, 7.5 & 8.5; pH 7.5; pH 5.5 & 7.5; pH 6.5, 7.5 & 8.5) indicated formation of a stoichiometric 1:1 complex of PMEIs with endogenous PMEs but also with enzymes from other species [ 41 , 53 , 56 , 57 ]. For example, Jolie et al [ 58 ] showed that purified PMEI from kiwi can bind to PMEs from carrot and broccoli (at pH 6.5), whereas tomato and Arabidopsis PMEIs can interact and inhibit PMEs from orange (at pH 7.5 and pH 7, respectively) [ 30 , 59 ]. On the other hand, plant PMEIs do not seem capable of inhibiting fungal or bacterial PMEs, as was shown for PMEI1 and PMEI2 from Arabidopsis, which did not affect PME activity of Botrytis cinerea [ 26 ].…”

Section: Pme Inhibitor Structure and Interaction With Pectin Estermentioning

confidence: 99%

“…In addition, hydrolysis of partially de-methylesterified HG can lead to the formation of signaling molecules (oligogalacturonides), for instance, during plant-pathogen interactions [ 26 , 27 ]. Consequently, PME activity is tightly regulated at: (a) the transcriptional level [ 28 ], (b) by protein processing [ 1 ] and degradation [ 29 ], (c) by the pH of the cell wall environment [ 6 , 30 ], and (d) by endogenous inhibitor proteins called pectin methylesterase inhibitors (PMEI, Figure 1 ) [ 31 , 32 ]. The first PMEI was identified in kiwi fruit ( Actinidia deliciosa [ 33 ]) and to date several PMEIs have been investigated in different plant species.…”

Section: Introductionmentioning

confidence: 99%

The Multifaceted Role of Pectin Methylesterase Inhibitors (PMEIs)

Wormit

Usadel

2018

IJMS

166

126

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Plant cell walls are complex and dynamic structures that play important roles in growth and development, as well as in response to stresses. Pectin is a major polysaccharide of cell walls rich in galacturonic acid (GalA). Homogalacturonan (HG) is considered the most abundant pectic polymer in plant cell walls and is partially methylesterified at the C6 atom of galacturonic acid. Its degree (and pattern) of methylation (DM) has been shown to affect biomechanical properties of the cell wall by making pectin susceptible for enzymatic de-polymerization and enabling gel formation. Pectin methylesterases (PMEs) catalyze the removal of methyl-groups from the HG backbone and their activity is modulated by a family of proteinaceous inhibitors known as pectin methylesterase inhibitors (PMEIs). As such, the interplay between PME and PMEI can be considered as a determinant of cell adhesion, cell wall porosity and elasticity, as well as a source of signaling molecules released upon cell wall stress. This review aims to highlight recent updates in our understanding of the PMEI gene family, their regulation and structure, interaction with PMEs, as well as their function in response to stress and during development.

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“…In addition, the overexpression of PMEI limits the movement of tobamovirus (tobacco mosaic virus) in tobacco and Arabidopsis, and reduces plant susceptibility to the virus ( Lionetti et al, 2014 ). Arabidopsis PME3 and PMEI7 were shown to have overlapping expression patterns in the etiolated hypocotyls when undergoing HGA methylesterification during plant development ( Sénéchal et al, 2015 ). Overexpression of Arabidopsis PME5 and PMEI3 resulted in softer and harder shoot apical meristem cell walls, respectively ( Peaucelle et al, 2011 ).…”

Section: Cell Wall Remodeling In Heat Responsementioning

confidence: 99%

Pectin Methylesterases: Cell Wall Remodeling Proteins Are Required for Plant Response to Heat Stress

2018

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Heat stress (HS) is expected to be of increasing worldwide concern in the near future, especially with regard to crop yield and quality as a consequence of rising or varying temperatures as a result of global climate change. HS response (HSR) is a highly conserved mechanism among different organisms but shows remarkable complexity and unique features in plants. The transcriptional regulation of HSR is controlled by HS transcription factors (HSFs) which allow the activation of HS-responsive genes, among which HS proteins (HSPs) are best characterized. Cell wall remodeling constitutes an important component of plant responses to HS to maintain overall function and growth; however, little is known about the connection between cell wall remodeling and HSR. Pectin controls cell wall porosity and has been shown to exhibit structural variation during plant growth and in response to HS. Pectin methylesterases (PMEs) are present in multigene families and encode isoforms with different action patterns by removal of methyl esters to influencing the properties of cell wall. We aimed to elucidate how plant cell walls respond to certain environmental cues through cell wall-modifying proteins in connection with modifications in cell wall machinery. An overview of recent findings shed light on PMEs contribute to a change in cell-wall composition/structure. The fine-scale modulation of apoplastic calcium ions (Ca2+) content could be mediated by PMEs in response to abiotic stress for both the assembly and disassembly of the pectic network. In particular, this modulation is prevalent in guard cell walls for regulating cell wall plasticity as well as stromal aperture size, which comprise critical determinants of plant adaptation to HS. These insights provide a foundation for further research to reveal details of the cell wall machinery and stress-responsive factors to provide targets and strategies to facilitate plant adaptation.

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Tuning of Pectin Methylesterification

Cited by 56 publications

References 67 publications

Quantitative Early Auxin Root Proteomics Identifies GAUT10, a Galacturonosyltransferase, as a Novel Regulator of Root Meristem Maintenance

Quantitative Early Auxin Root Proteomics Identifies GAUT10, a Galacturonosyltransferase, as a Novel Regulator of Root Meristem Maintenance

The Multifaceted Role of Pectin Methylesterase Inhibitors (PMEIs)

Pectin Methylesterases: Cell Wall Remodeling Proteins Are Required for Plant Response to Heat Stress

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