Pharmacological study of anti-allergic activity of Syzygium cumini (L.) Skeels

Abstract

Myrtaceae is a plant family widely used in folk medicine and Syzygium and Eugenia are among the most important genera. We investigated the anti-allergic properties of an aqueous leaf extract of Syzygium cumini (L.) Skeels (SC). HPLC analysis revealed that hydrolyzable tannins and flavonoids are the major components of the extract. Oral administration of SC (25-100 mg/kg) in Swiss mice (20-25 g; N = 7/group) inhibited paw edema induced by compound 48/80 (50% inhibition, 100 mg/kg; P ≤ 0.05) and, to a lesser extent, the allergic paw edema (23% inhibition, 100 mg/kg; P ≤ 0.05). SC treatment also inhibited the edema induced by histamine (58% inhibition; P ≤ 0.05) and 5-HT (52% inhibition; P ≤ 0.05) but had no effect on plateletaggregating factor-induced paw edema. SC prevented mast cell degranulation and the consequent histamine release in Wistar rat (180-200 g; N = 7/group) peritoneal mast cells (50% inhibition, 1 µg/mL; P ≤ 0.05) induced by compound 48/80. Pre-treatment of BALB/c mice (18-20 g; N = 7/group) with 100 mg/kg of the extract significantly inhibited eosinophil accumulation in allergic pleurisy (from 7.662 ± 1.524 to 1.89 ± 0.336 x 10 6 /cavity; P ≤ 0.001). This effect was related to the inhibition of IL-5 (from 70.9 ± 25.2 to 12.05 ± 7.165 pg/mL) and CCL11/eotaxin levels (from 60.4 ± 8.54 to 32.8 ± 8.4 ng/mL) in pleural lavage fluid, using ELISA. These findings demonstrate an anti-allergic effect of SC, and indicate that its anti-edematogenic effect is due to the inhibition of mast cell degranulation and of histamine and serotonin effects, whereas the inhibition of eosinophil accumulation in the allergic pleurisy model is probably due to an impairment of CCL11/eotaxin and IL-5 production.

Anti-inflammatory polysaccharides of Azadirachta indica seed tegument

“…Besides, pre-treatment of animals with methysergide inhibited the edema elicited by serotonin in 84% (25.5±6.9 AUC) ( Figure 2C). Our data indicates that FI did not antagonize histamine receptors, but could stabilize mast cell membranes, preventing the release of its content (histamine and serotonin) stimulated by compound 48/80 (Brito et al, 2007). This data is supported in literature, showing that polysaccharide fractions of aerial parts of Viola odorata and Malva pusilla plants presented antiinflammatory activity (Drozdova & Bubenchikov, 2005).…”

Phytochemical Composition and Pleotropic Pharmacological Properties of Jamun, Syzygium Cumini Skeels

“…Administration of OVA to BALBc mice induced substantial accumulation of leukocytes, mononuclear cells and eosinophils in the pleural cavity, whereas pretreatment of these mice with Jamun leaf extract at 1 h before the OVA administration significantly suppressed the accumulation of eosinophils in the pleural cavity, indicating its anti-inflammatory action. 63 Moreover, study of the antiallergic effects of aqueous, methanol and methanol fraction of the aqueous extract of Jamun roots revealed that these extracts suppressed clonidine-induced catalepsy in mice by inhibiting the release of histamine triggered by mast cell degranulation. Finally, the administration of different root extracts of Jamun was shown to suppress milk-induced eosinophilia in mice.…”

Inhibitory effect of a new orally active cedrol-loaded nanostructured lipid carrier on compound 48/80-induced mast cell degranulation and anaphylactic shock in mice

“…Only a fraction of ~250,000 plant species have been studied for antiasthmatic activity till now. [14][15][16][17][18][19][20][21] WHO has reported that 80% people of world population believe in traditional medicine for their health management. 22 In our laboratory, we have screened a vast number of natural extract in the form of steam volatile oil in vitro for their inhibitory effect on mast cell degranulation and anaphylactic shock.…”