Effect of metabolic control on interferon-gamma and interleukin-10 production by peripheral blood mononuclear cells from type 1 and type 2 diabetic patients

Abstract

The objective of the present study was to evaluate the production of cytokines, interferon-γ (INF-γ) and interleukin-10 (IL-10), in cultures of peripheral blood mononuclear cells (PBMC) from type 1 and type 2 diabetic patients and to correlate it with inadequate and adequate metabolic control. We studied 11 type 1 and 13 type 2 diabetic patients and 21 healthy individuals divided into two groups (N = 11 and 10) paired by sex and age with type 1 and type 2 diabetic patients. The PBMC cultures were stimulated with concanavalin-A to measure INF-γ and IL-10 supernatant concentration by ELISA. For patients with inadequate metabolic control, the cultures were performed on the first day of hospitalization and again after intensive treatment to achieve adequate control. INF-γ levels in the supernatants of type 1 diabetic patient cultures were higher compared to type 2 diabetic patients with adequate metabolic control (P < 0.001). Additionally, INF-γ and IL-10 tended to increase the liberation of PBMC from type 1 and 2 diabetic patients with adequate metabolic control (P = 0.009 and 0.09, respectively). The increased levels of INF-γ and IL-10 released from PBMC of type 1 and 2 diabetic patients with adequate metabolic control suggest that diabetic control improves the capacity of activation and maintenance of the immune response, reducing the susceptibility to infections.

Correlation of Interleukin-10, Superoxide Dismutase (SOD), and Malondialdehyde (MDA) Levels with HbA1c in Pediatric Type 1 Diabetes Mellitus

“…A previous study reported that IFN-γ and IL-10 production in diabetic peripheral blood mononuclear cells were associated with metabolic control. This result indicated that good metabolic control in diabetic patients would improve activation and maintenance of the immune response and decrease vulnerability to infection [37].…”

Absence of occult hepatitis B virus infection in sera of diabetic children and adolescents following hepatitis B vaccination

“…18 Although it was shown that impairment of polymorphonuclear phagocytosis and reduction in granulocyte phagocytic capacity in IDDM persons with inadequate metabolic control lead to an increase in susceptibility to infection, these abnormalities are reversible after insulin therapy. [19][20][21][22][23][24] In previous studies, increased proliferation capacity of IDDM T-lymphocytes 25 and a tendency toward increased tumor necrosis factor-α and interleukin-6, 26 as well as increased levels of interferon-γ levels, 27 after metabolic control were reported. Ten years after HB vaccination, 70% of our IDDM patients had anti-HBs levels of <10 IU/L.…”