Correlation of Interleukin-10, Superoxide Dismutase (SOD), and Malondialdehyde (MDA) Levels with HbA1c in Pediatric Type 1 Diabetes Mellitus

Astari, Lina; Cahyono, Haryudi Aji; Widjajanto, Edi

doi:10.11594/jtls.07.03.15

Cited by 5 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…showed that IL-10 was negatively associated with HbA1c in type 1 diabetic patients. 47 Finally, we reported that the duration of diabetes might act as an independent predictor of PWV, with higher PWV values in those with a longer course of diabetes. Likewise, other authors found the duration of T1DM to be the strongest determinant for arterial stiffness, as measured by PWV.…”

Section: Discussionmentioning

confidence: 78%

Association between proinflammatory cytokines and arterial stiffness in type 1 diabetic adolescents

Reis,

Teixeira,

Cardoso

et al. 2024

Journal of Pediatric Endocrinology and Metabolism

View full text Add to dashboard Cite

Objectives Type 1 diabetes mellitus is considered a state of chronic low-grade inflammation and activation of the innate immune system, which is regulated by several proinflammatory cytokines and other acute-phase reactants. Arterial stiffness, a dynamic property of the vessels evaluated by the determination of pulse wave velocity (PWV), is increased in diabetic patients and is associated with microvascular and macrovascular complications of diabetes and higher cardiovascular risk. In the present study, we aimed to compare the proinflammatory state and arterial stiffness in diabetic and non-diabetic adolescents, and to characterize the association between these two parameters. Methods Twenty-three type 1 diabetic patients, aged 12–16 years, followed at a tertiary center, and 23 adolescents nonoverweighted healthy controls, from a Portuguese birth-cohort, were included in the present analysis. Anthropometry, blood pressure, glycemic control data, and lipid parameters were collected. Arterial stiffness was evaluated by carotid–femoral pulse wave velocity. Proinflammatory cytokines’ concentrations (TNF-α, IL-1β, IL-6, IL-10, IFN-γ, and GM-CSF) were quantified by multiplex immunoassays using a Luminex 200 analyzer. Results There were no statistically significant differences between the proinflammatory cytokines’ concentrations in the two groups. PWV [6.63 (6.23–7.07) vs. 6.07 (5.15–6.65) m/s, p=0.015] was significantly higher in the diabetic group. PWV was negatively correlated with GM-CSF (ρ=−0.437, p=0.037) in the diabetic group. A linear association was found between diabetes duration and PWV (with PWV increasing by 0.094 m/s (95 % confidence interval, 0.019 to 0.169) per month of disease duration). In the diabetic group, HbA1c was negatively correlated with IL-10 (ρ=−0.473, p=0.026). Negative correlations were also found between IL-10 and total, HDL, and LDL cholesterol only in the diabetic group. Conclusions Diabetic adolescent patients present higher PWV, when compared to their healthy counterparts, even though we could not find differences in the levels of several proinflammatory cytokines between the two groups. The negative correlation found between IL-10 and HbA1c might translate a protective counterbalance effect of this anti-inflammatory cytokine, which might also explain the negative correlations found with blood lipids. Further studies are needed to better clarify the association between arterial stiffness and the proinflammatory milieu of diabetes.

show abstract

Section: Discussionmentioning

confidence: 78%

Association between proinflammatory cytokines and arterial stiffness in type 1 diabetic adolescents

Reis,

Teixeira,

Cardoso

et al. 2024

Journal of Pediatric Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…Another study has also shown that an increase in SOD may suppress the process of melanogenesis through the Tyrosinase pathway [34]. The previous studies have shown that the cytokines IL-10, IF-γ, and TNF -with their cell receptors will phosphorylate NADPH in a program that results in activation of the transcription factor NF-κB pathway through the mediation of phosphoinositide-3-kinase, MAPK, ERK1/2 [16], [34], [35]. Activated NF-κB will migrate into the nucleus and initiate the translation and transcription of various antioxidant gene expressions, including SOD [8].…”

Section: Discussionmentioning

confidence: 98%

“…It has been reported that SOD is one of the key factors reducing melanin production caused by UV irradiation [8], [10], [30]. Another study has also shown that an increase in SOD may suppress the process of melanogenesis through the Tyrosinase pathway [34]. The previous studies have shown that the cytokines IL-10, IF-γ, and TNF -with their cell receptors will phosphorylate NADPH in a program that results in activation of the transcription factor NF-κB pathway through the mediation of phosphoinositide-3-kinase, MAPK, ERK1/2 [16], [34], [35].…”

Section: Discussionmentioning

confidence: 98%

Effect of Secretome-Hypoxia Mesenchymal Stem Cells on Regulating SOD and MMP-1 mRNA Expressions in Skin Hyperpigmentation Rats

Zukhiroh

Putra

Chodidjah

et al. 2022

Open Access Maced J Med Sci

View full text Add to dashboard Cite

BACKGROUND: Ultraviolet B (UVB) radiation is the main factor causing hyperpigmentation. MSC secretome contains bioactive soluble molecules such as cytokines and growth factors that can accelerate skin regeneration. However, the molecular role of the secretome in hyperpigmentation is still unclear. AIM: This study aimed to determine the effect of secretome hypoxia mesenchymal stem cells (S-HMSC) gel on the expression of superoxide dismutase (SOD) and matrix metalloproteinases (MMP-1) genes in skin tissue of hyperpigmented rats induced by UVB light exposure. MATERIALS AND METHODS: Experimental research with post-test only control group. The control, base gel, T1 and T2 groups were UVB irradiated 6 times in 14 days at 302 nm with an minimal erythema dose of 390 mJ/cm2, respectively, while sham group did not receive UVB exposure. T1 was given 100 uL of S-HMSC gel and T2 was given 200 uL of S-HMSC gel every day for 14 days, while base gel received base gel. On day 15, skin tissue was isolated and analyzed for SOD and MMP-1 expression using qRT-PCR. RESULTS: The relative expression of the SOD gene in the treatment group (P1 = 0.47 ± 0.20, P2 = 1.22 ± 0.47) increased with increasing dose compared to the control group (UVB = 0.05 ± 0.01, Base gel = 0.05 ± 0.02). The relative expression of the MMP-1 gene in the treatment group (T1 = 5.82 ± 1.16, T2 = 2.86 ± 1.57) decreased with increasing dose compared to the control group (Control = 10.10 ± 2.31, and Base gel = 9.55 ± 1.29). CONCLUSION: Administration of S-HMSC gel can increase SOD gene expression and decrease MMP-1 gene expression in skin tissue of hyperpigmented rats model induced by UVB light.

show abstract

“…(24,25) The cytokines IL-10, IF-γ and TGF-β with their cell receptors will program the phosphorylate NADPH which results in the activation of the transcription factor NF-κB pathway through to the mediation of phosphoinositide-3-kinase, MAPK, ERK1/2. (26) Activated NF-κB will migrate into the nucleus, initiate the translation and transcription from various antioxidant gene expressions, including TNF-α and IL-18.…”

Section: Discussionmentioning

confidence: 99%

Secretome hypoxia-mesenchymal stem cells decrease tumor necrosis factor-α and interleukin-18 in kidney of type 2 diabetes mellitus model rats

Irawan,

Putra,

Setyo

et al. 2023

Univ Med

View full text Add to dashboard Cite

Background Type 2 diabetes mellitus (T2DM) is a chronic disease that affects millions of people worldwide and associated with an increased risk of kidney damage caused by prolonged inflammation. Secretome hypoxia- mesenchymal stem cells (SH-MSCs) have been investigated as a potential therapy for kidney inflammation in T2DM, due to their immunomodulatory properties and ability to promote tissue repair. In this study, we investigated the effects of SH-MSCs on tumor necrosis α (TNF-á) and interleukin-18 (IL-18) in the kidney of the T2DM model rats. MethodsA post-test-only control group involving 24 male Wistar rats. The rats were treated with a high-fat diet (HFD) for 4 weeks and streptozotocin-nicotinamide with sucrose solution for 5 days to induce T2DM animal models. Rats were randomly divided into four groups: healthy, control, and groups treated with SH-MSCs T1 and T2, with doses of 250 µL and 500 µL, respectively. TNF-α and IL-18 gene expression was measured by real time polymerase chain reaction (RT-PCR). One Way ANOVA and post-hoc LSD tests were used to determine the significant difference against all groups based on their quantitative measurement. ResultsAdministration of the SH-MSCs at a dose of 500µL (T2) was able to significantly reduce TNF-α and IL-18 gene expression when compared to control (T2DM rat without treatment) (p<0.05), but not significantly when compared to healthy and SH-MSC at a dose of 250µL (T1) group (p >0.05). ConclusionThis study demonstrated that the SH-MSCs decreased the levels of proinflammatory cytokines TNF-α and IL-18 gene expression in the kidney of T2DM model rats.

show abstract

Correlation of Interleukin-10, Superoxide Dismutase (SOD), and Malondialdehyde (MDA) Levels with HbA1c in Pediatric Type 1 Diabetes Mellitus

Cited by 5 publications

References 30 publications

Association between proinflammatory cytokines and arterial stiffness in type 1 diabetic adolescents

Association between proinflammatory cytokines and arterial stiffness in type 1 diabetic adolescents

Effect of Secretome-Hypoxia Mesenchymal Stem Cells on Regulating SOD and MMP-1 mRNA Expressions in Skin Hyperpigmentation Rats

Secretome hypoxia-mesenchymal stem cells decrease tumor necrosis factor-α and interleukin-18 in kidney of type 2 diabetes mellitus model rats

Contact Info

Product

Resources

About