Short-term evaluation of non-absorbable microgranular hydroxyapatite infiltration in the guinea pig subepidermal abdominal region

Xavier, Adriana P.; Schellini, Silvana Artioli; Aragon, Flávio Ferrari; Padovani, Carlos Roberto; Taga, Eulázio Mikio

doi:10.1590/s0100-879x2002000100010

Cited by 3 publications

(4 citation statements)

References 3 publications

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“…These results indicated a predominance of mononuclear cells in samples from eight days and tissue organization around the biomaterial with tendency for encapsulation with ever-smaller portions of HAP-91 ® , as well as fibrous capsule formation and mild inflammatory reaction, being observed in samples from 21 and 49 days, as reported by Li et al (1999), Xavier et al (2002), Berlin et al (2006), Carlo et al (2007), Dayan & Bassichis (2008), Vogt et al (2008), Jones (2009), Qu et al (2010 and Khashaba et al (2011).…”

Section: Resultssupporting

confidence: 78%

“…The fibrous capsule formation is related to a number of factors such as the size, shape and texture of the implant as well as its chemical properties, porosity, deployment location, and the physical and chemical stimuli caused by the implant, being an expected response to the material within the normal range (Li et al, 1999;Carlo et al, 2007;Qu et al, 2010;Khashaba et al, 2011). According to Xavier et al (2002), it is likely that this capsule prevents the leakage of the biomaterial, therefore reducing the inflammatory response near the site of application and thereby reducing the occurrence of granulomas.…”

Section: Resultsmentioning

confidence: 99%

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Composite synthetic hydroxyapatite 30%, in two physical states, as dermal filler

et al. 2013

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RESUMO Compósito de hidroxiapatita sintética 30%, em dois estados físicos, como preenchedor de relevo cutâneoObjetivou-se avaliar a resposta tecidual, após implantação de hidroxiapatita sintética 30% (HAP-91 ® ), em dois estados físicos, para preenchimento cutâneo. Foram utilizados 18 coelhos da raça Nova Zelândia, distribuídos aleatoriamente em dois grupos ® fluida e viscosa) e, posteriormente, divididos em três subgrupos, de acordo com o período pós-implante, em oito, 21 e 49 dias. Em cada grupo foi implantado 1,0 mL de HAP-91 ® , fluida ou viscosa, no tecido subcutâneo, 1 cm cranial à crista da escápula direita. Foi realizada a mensuração da espessura cutânea, imediatamente antes e durante 15 dias após a implantação do compósito. Avaliações de sensibilidade dolorosa foram realizadas, atribuindo-se os escores: 0 -quando o animal permitiu o toque e não manifestou reação; 1 -quando permitiu o toque, mas manifestou reação de dor, como aumento da frequência respiratória ou tentativa de fuga; 2 -quando não permitiu o toque. Aos oito, 21 e 49 dias, foi realizada a biópsia da região implantada. Não foi observada diferença entre a espessura cutânea dos animais (p > 0,05) e todos os animais receberam escore 0 para sensibilidade dolorosa. Na análise histológica, não foi notado processo inflamatório evidente. Houve predomínio de células mononucleares, nas amostras de oito dias, e uma organização tecidual ao redor do biomaterial, com tendência ao encapsulamento. A HAP-91 ® , tanto fluida quanto viscosa, é biocompatível e indicada para o preenchimento cutâneo.Palavras-chave: implante cutâneo, compósito, medicina estética. The aim of this study was to evaluate the response to the implantation of synthetic hydroxyapatite 30% (HAP-91 ® ) in different physical states as dermal filler. Eighteen New Zealand rabbits were used, distributed randomly into two equal groups and then divided into three groups according to the postoperative period at 8, 21 and 49 days. One mL of HAP-91 ® , fluid and viscous, was implanted in the subcutaneous tissue, 1 cm proximal to the cranial crest of the right scapula. The thickness of the skin was measured before and after implantation and for the following 15 days. Pain sensitivity assessment was conducted, assigning the following scores: 0 -when the animal allowed the touch of the implant area and expressed no signs of pain; 1 -when the animal allowed the touch, but pain reaction occurred, like increase of the respiratory rate or attempt to escape; 2 -when the animal did not allow the touch to the implanted area. At 8, 21 and 49 days, biopsy of the implanted area was performed. No difference was observed between the thickness of the skin (p>0.05) and all animals received a score 0 for soreness. Histological analysis did not reveal any obvious inflammatory process, showing a predominance of mononuclear cells in samples of eight days and tissue organization around the biomaterial with a tendency to encapsulation. The results indicate that HAP-91 ® , both viscous and fluid, is biocompatible...

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Composite synthetic hydroxyapatite 30%, in two physical states, as dermal filler

et al. 2013

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“…A long technological challenge has been traversed until the production method of Nanoskin, material resulting from the synthesis process and which, thus, is not limited, as it would be in the case of other materials such as natural hydroxyapatite. 3 The strong initial appeal of integrated implants 3 was the starting point so that new materials were studied [4][5][6] , opening a wide range of research seeking for the ideal biomaterial. The present study showed that Nanoskin is a biomaterial that can be well tolerated by the orbital cavity, not having caused any death or showing that the overall health of the animal had been affected, despite the need for biochemical and histology exams of target organs to confirm that.…”

Section: Discussionmentioning

confidence: 99%

Use of Nanoskin for volume replacement of the eye socket

Mussi¹,

Orsolini²,

Pellizon³

et al. 2016

Revista Brasileira De Oftalmologia

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Objective: The aim of this study was to evaluate the biocompatibility of Nanoskin for replacing volume in enucleated or eviscerated anophthalmic sockets of rabbits. Methods: An experimental study was carried out using enucleated or eviscerated rabbits, which received Nanoskin implants (Innovatecs®, São Carlos, Brazil), a cellulose produced by a bacteria (Acetobacter xylinum) using green tea as substrate. Implants of 10mm diameter/5mm of thickness were used placed in enucleated (G1) or eviscerated (G2) anophthalmic sockets of 21 rabbits. They were clinically examined daily, sacrificed at 7, 30 and 90 days after surgery and the material was removed and prepared for histological examination. Results: There were discrete signs of inflammation in the immediate postoperative period, with no evidence of infection or extrusion in any animal. However apparent reduction of volume during the trial period occurred. Histologically both groups were similar, with inflammatory cells (mainly monocytes and neutrophils), fibrin and hemaceas at 7 days postoperatively. The Nanoskin was presented as small pink spheres, with small gaps between them and permeated by few inflammatory cells. These cells have changed over the study, at 30 days multinucleated giant cells and mature fibroblasts that permeate the implant were observed. At 90 days, the structure of the implant was disorganized, amorphous, with necrotic debris and ovoid areas covered with thin pink membrane that seemed to cluster, empty or filled with no cellular pink or gray material. Conclusion: Nanoskin caused an inflammatory reaction leading to reabsorption and reduction of implant volume. New formulations should be studied in order to have a permanent product to repair the anophthalmic socket.

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“…Alguns estudos analisaram implantes de materiais gelatinosos, como o polietileno gel injetado no subcutâneo de cobaias (6,7) e esferas de polietileno gel, colocados em cavidades anoftálmicas de coe lhos (4) , ambos com boa resposta tecidual. Implantes não rígidos, feitos de silicone no estado gelatinoso já foram largamente utilizados em outros locais do corpo humano, como os implantes mamários, em mastoplastias de aumento (8) .…”

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Estudo comparativo entre as esferas de silicone gelatinosas e rígidas em cavidade eviscerada de coelho

Kanamura¹,

Padovani²,

Pereira³

et al. 2012

Arq. Bras. Oftalmol.

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INTRODUÇÃOAs técnicas de enucleação e evisceração geralmente requerem reposição de conteúdo orbitário para minimizar o déficit de volume e, consequentemente, assimetrias e prejuízos estéticos (1)(2)(3) . Para repor o volume orbitário perdido, podem ser utilizados materiais autólo-gos, homólogos, heterólogos ou aloplásticos.Materiais aloplásticos são os mais utilizados, podendo-se implantar esferas confeccionadas com diversas substâncias, como polimetilmetacrilato, silicone, hidroxiapatita sintética, polietileno poroso (4) , bio vidro e biocerâmica (5) . Todos os implantes aloplásticos que existem atualmente para re construção da cavidade anoftálmica são rígidos, assim como a pró tese externa. O movimento constante dos músculos extrínsecos e das pálpebras pode fazer com que a prótese externa tenha contatos traumáticos com o implante orbitário, propiciando pontos de RESUMO Objetivo: Avaliar e comparar a biocompatibilidade de esferas de silicone gelatinosas e rígidas em cavidades evisceradas de coelhos. Métodos: Trinta coelhos tiveram o olho direito eviscerado com implantação de esferas de silicone gelatinosas (Grupo I) ou rígidas (Grupo II). Foi realizada avaliação clínica diária, ultrassonografia da cavidade orbitária, análise histológica e morfométrica da pseudocápsula que se formou ao redor dos implantes aos 7, 30 e 90 dias após a cirurgia, com avaliação estatística dos resultados. Resultados: Houve boa integração das esferas com os tecidos orbitários e semelhança de resposta tecidual com ambas as esferas. Duas esferas de silicone gelatinosas e uma rígida extruíram. A pseudocápsula que se formou ao redor das esferas gelatinosas foi mais organizada, com espessura e reação inflamatória menores que a observada nas esferas rígidas. Conclusões: Esferas de silicone gelatinosas e rígidas tiveram boa integração tecidual em cavidades evisceradas de coelhos. Descritores

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Short-term evaluation of non-absorbable microgranular hydroxyapatite infiltration in the guinea pig subepidermal abdominal region

Cited by 3 publications

References 3 publications

Composite synthetic hydroxyapatite 30%, in two physical states, as dermal filler

Composite synthetic hydroxyapatite 30%, in two physical states, as dermal filler

Use of Nanoskin for volume replacement of the eye socket

Estudo comparativo entre as esferas de silicone gelatinosas e rígidas em cavidade eviscerada de coelho

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