Genomic characterization of Brazilian hepatitis C virus genotypes 1a and 1b

Abstract

Parts of 5' non-coding (5' NC) and of E1 envelope regions of the hepatitis C virus (HCV) genome were amplified from sera of 26 Brazilian anti-HCV antibody-positive patients using the reverse transcription-polymerase chain reaction (RT-PCR). Fourteen samples were PCR positive with primers from the 5' NC region and 8 of them were also positive with primers from the E1 region. A genomic segment of 176 bp from the E1 region of 7 isolates was directly sequenced from PCR products. The sequences were compared with those of HCV strains isolated in other countries and the Brazilian isolates were classified by phylogenetic analysis into genotypes 1a and 1b. This could have a clinical importance since it has been shown that individuals infected with type 1 viruses are less likely to respond to treatment with interferon than individuals infected with types 2 and 3 viruses. Two quasispecies isolated from the same patient with an interval of 13 months differed by two base substitutions (1.1%). The sequence of another isolate presented a three-nucleotide deletion at codon 329.

[No Title Available]

“…Detection and genotyping of HCV RNA -All samples were submitted to RNA extraction, reverse transcription, and a nested PCR with primers complementary to the conserved area of the 5' non-coding region of HCV, essentially as described by Ginabreda et al (1997). Positive samples were genotyped by line probe assay (INNO-LiPA HCV, Innogenetics NV, Ghent, Belgium).…”

Hepatitis C virus genotypes in hemodialysis patients in the Federal District, Brazil

“…Sixty-six anti-HCV positive samples were subjected to RNA extraction by the commercially standardized reagents (QIAamp Viral RNA Mini Kit, Qiagen, Hilden, Germany) according to the manufacturer's instructions, followed by reverse transcription, as described by GINABREDA et al 11 . cDNA was amplified using the primers complementary to the conserved area of the 5' NC and NS5B regions and PCR conditions described by GINABREDA et al 11 and SANDRES-SAUNÉ et al 21 , respectively.…”

“…cDNA was amplified using the primers complementary to the conserved area of the 5' NC and NS5B regions and PCR conditions described by GINABREDA et al 11 and SANDRES-SAUNÉ et al 21 , respectively.…”

Prevalence of hepatitis C Virus infection among hemophiliacs in Central Brazil

“…Positive samples were retested for confirmation using a line immunoassay (INNO-LIA HCV Ab III, Innogenetics). All samples were submitted to RNA extraction, reverse transcription, and a nested PCR with primers complementary to the conserved area of the 5' non-coding region of HCV, essentially as describe by Ginabreda et al (1997). Prevalence and 95% confidence intervals (95% CI) were calculated.…”