Resistance of Amaranthus retroflexus to acetolactate synthase inhibitor herbicides in Brazil

Francischini, Alessandra Constantin; Constantin, Jamil; Oliveira, Rubem Silvério de; Santos, Gizelly; Franchini, Luiz Henrique Morais; Biffe, Denis Fernando

doi:10.1590/s0100-83582014000200022

Cited by 19 publications

(17 citation statements)

References 20 publications

(15 reference statements)

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“…A summary of resistance reports confirmed in this work is found in Table 4. Biotype MT13 showed resistance in preemergence, but not in postemergence, considering the results found in previous studies (Francischini et al, 2014b) (Table 3). In the present work, three biotypes were identified with multiple resistance to ALS-and PS II inhibitors (GO3, GO5 and MT13), which further increases limits of control options for this species in cotton fields.…”

Section: Als Inhibitors (Trifloxysulfuron-sodium and Pyrithiobac-sodium)supporting

confidence: 78%

“…In Brazil, the most recent cases include A. retroflexus resistant to protoporphyrinogen oxidase (PPO) inhibitors (Heap, 2019) and A. palmeri, simultaneously resistant to glyphosate and ALS inhibitors (Gonçalves Netto et al, 2016). In cotton fields, A. retroflexus populations resistant to trifloxysulfuron-sodium and pyrithiobac-sodium were found (Francischini et al, 2014b) as well as A. viridis resistant to trifloxysulfuron-sodium (Francischini et al, 2014a), applied in postemergence.…”

Section: Introductionmentioning

confidence: 99%

“…In previous crop seasons in the states of Goiás, Mato Grosso do Sul and Mato Grosso, poor control of redroot pigweed (A. retroflexus) was observed for herbicides used in cotton preemergence, such as ALS and PS II inhibitors. Therefore, the objective of this research was to evaluate the occurrence of cross resistance to ALS-and PS II-inhibiting herbicides in redroot pigweed (A. retroflexus) biotypes, studied by Francischini et al (2014b), but this time with preemergence applications of products used in cotton fields.…”

Section: Introductionmentioning

confidence: 99%

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Multiple-and Cross-Resistance of Amaranthus retroflexus to Acetolactate Synthase (ALS) and Photosystem II (PSII) Inhibiting Herbicides in Preemergence

et al. 2019

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Herbicide resistance in Amaranthus genus occurs frequently around the word and has become a big problem in cotton producing areas. The objective of this work was to evaluate cross-and multiple-resistance of redroot pigweed (A. retroflexus) to herbicides used in preemergence in cotton fields in Brazil. Seven dose-response experiments were conducted with herbicides atrazine, prometryn, diuron, S-metolachlor, trifluralin, trifloxysulfuron-sodium and pyrithiobac-sodium, and the treatments consisted of application rates of 0, ¼, ½, 1, 2 and 4 times the recommended label rate. Eight A. retroflexus byotipes with suspect of resistance were sampled for experiments in three brazilian states of cotton producing. Resistance to prometryn was confirmed for one biotype in Goiás (GO), and one biotype from Mato Grosso (MT) showed cross-resistance to atrazine and prometryn. One byotipe from GO was identified with cross-resistance to trifloxysulfuron-sodium and pyrithiobac-sodium. One of the GO samples was identified with multiple resistance to prometryn and ALS inhibitors, another one to atrazine and ALS inhibitors, while MT byotipe was confirmed with multiple resistance to triazines and pyrithiobac. The herbicides S-metolachlor, diuron, and trifluralin were efficient for control of this species, therefore, they can be used as managment alternative in those regions.Keywords: redroot pigweed, Gossypium hirsutum L., triazines, sulfonylureas. RESUMO -A resistência a herbicidas em espécies do gêneroAmaranthus ocorre frequentemente no mundo e está se tornando um grande problema em regiões algodoeiras no Brasil. O objetivo deste trabalho foi verificar a resistência cruzada e múltipla de biótipos de caruru-gigante (A. retroflexus) aos herbicidas utilizados em pré-emergência na cultura do algodão no Brasil. Sete experimentos de doseresposta foram realizados em casa de vegetação com os herbicidas atrazine, prometryn, diuron, S-metolachlor, trifuralin, trifloxysulfuron-sodium e pyrithiobacsodium, sendo os tratamentos equivalentes a 0, ¼, ½, 1, 2 e 4 vezes a dose recomendada. Foram avaliados oito biótipos de A. retroflexus coletados em três Estados produtores de algodão do Brasil com suspeita de resistência a esses herbicidas. Confirmou-se a resistência em um biótipo de Goiás (GO) para prometryn, assim como um de Mato Grosso (MT), que apresentou resistência cruzada a atrazine e prometryn. Um biótipo do Estado do Mato Grosso do Sul (MS) e quatro de GO apresentaram resistência cruzada a trifloxysulfuron-sodium e pyrithiobac-sodium. Um biótipo de GO foi identificado com resistência múltipla a prometryn e inibidores Planta Daninha 2019; v37:e019179353 FRANCISCHINI, A. et al. Multiple-and cross-resistance of Amaranthus retroflexus to acetolactate synthase (ALS) and ... 2 da ALS, outro para atrazine e inibidores da ALS, enquanto para o bipótipo do MT foi confirmada resistência múltipla para triazinas e pirimiditio-benzoatos (pyrithiobac). Os herbicidas S-metolachlor, diuron e trifuralin foram eficientes no controle de todas as populações e, portan...

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Section: Als Inhibitors (Trifloxysulfuron-sodium and Pyrithiobac-sodium)supporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple-and Cross-Resistance of Amaranthus retroflexus to Acetolactate Synthase (ALS) and Photosystem II (PSII) Inhibiting Herbicides in Preemergence

et al. 2019

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“…Previously, redroot pigweed accessions/biotypes/populations resistant to one or more ALSinhibiting herbicides, including those evaluated in this research, have been reported from Brazil, Canada, China, Germany, Israel, Italy, Serbia, and the USA [5,[19][20][21]. The resistance level of the RRPW-R population to pyrithiobac, 1476-fold, is higher compared to resistance factors of 3 to 71 [19] and 7 to 38 [20] reported earlier for redroot pigweed from Brazil. The resistance index of 900 to trifloxysulfuron in the RRPW-R population was higher compared to redroot pigweed populations from Brazil that exhibited R/S values of 23 to 58 in 2014 [19] and 339-fold levels reported in 2019 [20].…”

Section: Discussionmentioning

confidence: 99%

Resistance to acetolactate synthase inhibitors is due to a W 574 to L amino acid substitution in the ALS gene of redroot pigweed and tall waterhemp

Nandula

Giacomini

Ray³

2020

PLoS ONE

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Several Amaranthus spp. around the world have evolved resistance (and cross resistance) to various herbicide mechanisms of action. Populations of redroot pigweed (RRPW-R) and tall waterhemp (TW-R) in Mississippi, USA have been suspected to be resistant to one or more acetolactate synthase (ALS) inhibiting herbicides. Whole plant dose-response experiments with multiple ALS inhibitors, ALS enzyme assays with pyrithiobac, and molecular sequence analysis of ALS gene constructs were conducted to confirm and characterize the resistance profile and nature of the mechanism in the RRPW-R and TW-R populations. Two susceptible populations, RRPW-S and TW-S were included for comparison with RRPW-R and TW-R, correspondingly. The resistance index (R/S; the herbicide dose required to reduce plant growth by 50% of resistant population compared to the respective susceptible population) values of the RRPW-R population were 1476, 3500, and 900 for pyrithiobac, imazaquin, and trifloxysulfuron, respectively. The R/S values of the TW-R population for pyrithiobac, imazaquin, and trifloxysulfuron were 51, 950, and 2600, respectively. I 50 values of RRPW-S and RRPW-R populations for pyrithiobac were 0.062 and 208.33 μM, indicating that the ALS enzyme of the RRPW-R population is 3360-fold more resistant to pyrithiobac than the RRPW-S population under our experimental conditions. The ALS enzyme of the TW-R population was 1214-fold resistant to pyrithiobac compared to the TW-S population, with the I 50 values for pyrithiobac of ALS from TW-R and TW-S populations being 87.4 and 0.072 μM, correspondingly. Sequencing of the ALS gene identified a point mutation at position 574 of the ALS gene leading to substitution of tryptophan (W) residue with a leucine (L) residue in both RRPW-R and TW-R populations. Thus, the RRPW-R and TW-R populations are resistant to several ALS-inhibiting herbicides belonging to different chemical classes due to an altered target site, i.e., ALS. Resistance in Amaranthus spp. to commonly used ALS-inhibiting herbicides warrants an

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“…For the purpose of this work, the populations were considered resistant when RF≥2.0 and simultaneously satisfied two additional conditions: C 80 and GR 80 values > highest recommended dose to control this species (Francischini et al, 2014;Santos et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Multiple resistance to atrazine and imazethapyr in hairy beggarticks (Bidens pilosa )

et al. 2016

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Resistance to herbicides is a serious threat to crop production worldwide, especially in agronomic crops and cereals. This research evaluated the possible occurrence of Bidens pilosa resistant to imazethapyr and atrazine in Brazil. The resistant biotype was collected from an area with a history of repeated application of photosystem II (PSII) and ALS inhibitor herbicides. The susceptible biotype was collected from an area with no history of herbicide application. Resistance verification experiments were carried out in the greenhouse. The treatments were arranged in a 3 x 8 factorial scheme, where the first factor was populations [susceptible (S), parent resistant (PR), and resistant F1 (RF1)]; and the second factor was herbicide dose (0, 375, 750, 1500, 3000, 6000, 12000 and 24000 g ha -1 for atrazine; or 0, 12.5, 25, 50, 100, 200, 400 and 800 g ha -1 for imazethapyr). The resistance factor to atrazine was 2.83 for PR and 5.55 for RF1. This population was more resistant to imazethapyr (>21-fold) than it was to atrazine. The recommended maximum dose of the herbicides did not control this B. pilosa population adequately. The data support the claim that B. pilosa population from this field in Quarto Centenário, Parana is resistant to two herbicide modes of action -PSII inhibitor (i.e. atrazine) and ALS inhibitor (i.e. imazethapyr). This is the first report of such case for this species, globally. Cross-resistance to other ALS inhibitors and other PS II inhibitors as well as the respective mechanisms of resistance to each herbicide are being investigated.Index terms: Photosystem II; resistance level; herbicide resistance. RESUMOA resistência de plantas daninhas à herbicidas constitui uma das principais ameaças para a agricultura no mundo, especialmente para a produção de grãos. O presente trabalho objetivou verificar a possível existência de Bidens pilosa resistente a imazethapyr e atrazina. Dois experimentos foram realizados em casa-de-vegetação, sendo um para cada herbicida. O biótipo resistente foi proveniente de área com histórico de aplicações de herbicidas inibidores do fotossistema II e da ALS, enquanto que o biótipo suscetível foi coletado em área sem histórico de aplicação de herbicidas. Os tratamentos foram dispostos em esquema fatorial 3 x 10, em que o primeiro fator foi composto por diferentes populações [suscetível (S), resistente parental (RP) e resistente F1 (RF1)], e o segundo fator constituiu de doses de cada herbicida (0, 375, 750, 1500, 3000, 6000, 12000 and 24000 g i.a. ha -1 para atrazina e 0; 12,5; 25; 50; 100; 200; 400 e 800 g e.a. ha -1 para imazethapyr). O fator de resistência variou entre 2,83 e 5,55 para atrazina e >21 para imazethapyr. Além disso, a dose máxima de registro dos herbicidas não controlou as plantas do biótipo resistente. Os resultados revelaram que a população de B. pilosa proveniente de Quarto Centenário, Paraná, apresenta resistência múltipla a dois mecanismos de ação: inibidores da ALS (atrazine) e do fotossistema II (imazethapyr). Este é o primeiro relato deste t...

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Resistance of Amaranthus retroflexus to acetolactate synthase inhibitor herbicides in Brazil

Cited by 19 publications

References 20 publications

Multiple-and Cross-Resistance of Amaranthus retroflexus to Acetolactate Synthase (ALS) and Photosystem II (PSII) Inhibiting Herbicides in Preemergence

Multiple-and Cross-Resistance of Amaranthus retroflexus to Acetolactate Synthase (ALS) and Photosystem II (PSII) Inhibiting Herbicides in Preemergence

Resistance to acetolactate synthase inhibitors is due to a W 574 to L amino acid substitution in the ALS gene of redroot pigweed and tall waterhemp

Multiple resistance to atrazine and imazethapyr in hairy beggarticks (Bidens pilosa )

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