Pseudomonas spp.: contamination sources in bulk tanks of dairy farms

Vidal, Ana Maria Centola; Netto, Arlindo Saran; Vaz, Andréia Cristina Nakashima; Capodifoglio, Eduardo; Gonçalves, Ana Carolina Siqueira; Rossi, Gabriel Augusto Marques; Figueiredo, Annanda Souza de; Ruiz, Vera Letticie de Azevedo

doi:10.1590/s0100-736x2017000900008

Cited by 14 publications

(8 citation statements)

References 24 publications

(35 reference statements)

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“…Pseudomonas sp. is widespread in the environment of dairy cows where the major sources in farms are multiple water supplies, soil, contaminated teat dips and infusion equipment [ 13 ]. The antibiotic recalcitrance associated with Pseudomonas can be mainly attributed to the formation of biofilms in different surfaces [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

et al. 2020

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Bovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventional drugs. Pseudomonas sp. is one of the pathogens involved in this infection. The therapeutic rate of cure for this environmental mastitis-causing pathogen is practically zero, regardless of treatment. Biofilm formation has been one of the main virulence mechanisms of Pseudomonas hence presenting resistance to antibiotic therapy. We have manufactured chitosan nanoparticles (NQo) with tripolyphosphate (TPP) using ionotropic gelation. These NQo were confronted against a Pseudomonas sp. strain isolated from milk samples of cows diagnosed with BM, to evaluate their antimicrobial and antibiofilm capacity. The NQo showed great antibacterial effect in the minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC) and disk diffusion assays. Using sub lethal concentrations, NQo were tested for inhibition of biofilm formation. The results show that the nanoparticles exhibited biofilm inhibition and were capable of eradicate pre-existing mature biofilm. These findings indicate that the NQo could act as a potential alternative to antibiotic treatment of BM.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

et al. 2020

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“…Tais resultados podem ser atingidos por qualquer indústria de laticínios por meio da adoção de boas práticas de ordenha e refrigeração adequada do leite cru. Sabe-se que a presença de bactérias psicrotróficas, como a Pseudomonas spp., pode afetar negativamente o processamento térmico de derivados lácteos, portanto, boas práticas higiênicas podem contribuir diretamente para a melhoria da qualidade desses produtos (VIDAL et al, 2017). VIDAL et al (2017), procurando as principais fontes de contaminação por bactérias psicrotróficas em tanques de expansão de dez fazendas leiteiras, verificaram que a má higienização da mão de ordenhadores, da superfície dos tetos das vacas, das teteiras e dos tanques de resfriamento são as principais causas da presença das bactérias no leite cru.…”

Section: Resultsunclassified

Microrganismos Psicrotróficos Em Leites Submetidos À Ultra Alta Temperatura Adquiridos Em Curitiba, Paraná

Souza

Rosa

Galvão

2021

Ars Vet

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O tratamento do leite em ultra alta temperatura (UAT) possibilita a eliminação de patógenos e aumento do tempo de prateleira, no entanto, bactérias psicrotróficas são associadas a efeitos negativos no produto, como lipólise, rancificação e a geleificação. Resultado da resistência de alguns esporos ao processamento, e à possível atividade residual de enzimas termorresistentes. Assim, o objetivo deste estudo foi enumerar bactérias psicrotróficas e realizar a prova de lactofermentação em leites UAT comercializados na região de Curitiba, Paraná. Foram adquiridas 60 amostras de duas marcas (30 da marca A e 30 da marca B) quinzenalmente em três diferentes momentos. Em cada momento 10 amostras de um mesmo lote eram separadas em dois grupos (D0 e D7), sendo D0: cinco amostras avaliadas no dia da compra, e D7: cinco amostras avaliadas após incubação a 37± 1ºC/7 dias e analisadas quanto à enumeração de bactérias psicrotróficas, coloração de Gram e prova da lactofermentação. Em D0, uma (3,4%) amostra (marca A) apresentou crescimento de psicrotróficos (2,50 x 10 5 UFC/mL), Gram negativos. As amostras da marca B não apresentaram crescimento microbiano. Vinte e cinco (83,3%) amostras não formaram coágulo, e cinco (16,7%) produziram coágulo esponjoso. Em D7, nenhuma amostra apresentou contagem bacteriana, 20 (66,7%) não produziram coágulo e 10 (33,3%) produziram coágulo esponjoso. O processo UAT utilizado pela marca A não garantiu efetividade na redução de bactérias psicrotróficas, e o resultado do teste de lactofermentação indica a possível contaminação por coliformes em ambas as marcas. Ainda, a ausência de coágulos em diversas amostras pode ser um indicativo do uso de conservantes, o que é considerado fraude.

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“…Milkers' hands, surface of cows' teats, teat cups and cooling tanks were associated with raw milk contamination with Pseudomonas spp. on farms with manual and mechanical milking system, showing that regardless of the type of milking system and season, proper hygiene procedures of equipment, utensils and workers' hands are essential to avoid contamination of raw milk (Vidal et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk

et al. 2018

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Pseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat’s and cow’s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk.

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Pseudomonas spp.: contamination sources in bulk tanks of dairy farms

Cited by 14 publications

References 24 publications

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

Microrganismos Psicrotróficos Em Leites Submetidos À Ultra Alta Temperatura Adquiridos Em Curitiba, Paraná

Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk

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