Toxicity and oxidative stress of canine mesenchymal stromal cells from adipose tissue in different culture passages

Abstract

Stem cells in regenerative therapy have received attention from researchers in recent decades. The culture of these cells allows studies about their behavior and metabolism. Thus, cell culture is the basis for cell therapy and tissue engineering researches. A major concern regarding the use of cultivated stem cell in human or veterinary clinical routine is the risk of carcinogenesis. Cellular activities require a balanced redox state. However, when there is an imbalance in this state, oxidative stress occurs. Oxidative stress contributes to cytotoxicity, which may result in cell death or genomic alterations, favoring the development of cancer cells. The aim of this study was to determine whether there are differences in the behavior of cultured mesenchymal stem cells from canine adipose tissue according to its site of collection (omentum and subcutaneous) evaluating the rate of proliferation, viability, level of oxidative stress and cytotoxicity over six passages. For this experiment, two samples of adipose tissue from subcutaneous and omentum where taken from a female dog corpse, 13 years old, Pitbull. The results showed greater levels of oxidative stress in the first and last passages of both groups, favoring cytotoxicity and cell death.

Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais

“…A mensuração da viabilidade e obtenção celular foi realizada nas três expansões do cultivo celular das CTPDC. Foram utilizados métodos padrões de avaliação em câmara de contagem celular (Sprada et al, 2015). As análises estatísticas foram realizadas por ANOVA seguido do teste TUKEY, quando comparado mais de dois grupos.…”

“…Karaöz et al (2010), relataram índices de viabilidade semelhantes em culturas de células obtidas da polpa dentária humana, sugerindo semelhança no padrão celular entre estas espécies. Sprada et al (2015), também demonstraram padrões aproximados de crescimento e viabilidade de células-tronco mesenquimais adipoderivadas caninas, o que evidencia as características semelhantes das CTIPDC e CTMs, não só em aspecto morfológico e padrões imunofenotípicos, mas também relativo aos valores de crescimento, proliferação e viabilidade celular.…”

Células-tronco mesenquimais de origem adiposa na fase de proliferação do processo de cicatrização de queimaduras frias

“…As ADSCs foram isoladas da gordura intraabdominal perigonadal de um rato Wistar macho, hígido, com 60 dias, após a eutanásia por sobredose anestésica de isoflurano. O processamento e o cultivo das ADSCs ocorreram conforme protocolo já estabelecido (Sprada et al, 2015). As células foram diferenciadas na terceira passagem (P3) em adipócitos e osteócitos, de acordo com o protocolo de Deus et al (2012).…”

Renal Progenitor Cells Have Higher Genetic Stability and Lower Oxidative Stress than Mesenchymal Stem Cells during In Vitro Expansion

“…Contemporary studies have identified that early passages, until the third passage, maintain the intrinsic characteristics of prolificity, viability, plasticity, antioxidant, and immunomodulation of some cell lines [4][5][6]. However, these observations may vary according to the sources of production and the lines studied, which raises relevant questions about the effect of growing time on genetic stability [7][8][9][10].…”

“…It seems evident to us that the preservation of the antioxidant system may have contributed to the lower rates of lipid peroxidation and nitrite production, as well as the lower average of DNA damage index and frequency, observed previously in this study. Previous studies had already described such in vitro behavior, in which stem cell lineages in early passages exhibited high resistance to necrosis induced by oxidative stress, mediated by the cellular capacity to reduce intracellular production of reactive oxygen species, as well as preserve the constitutive expression of the antioxidant system [4,50].…”