A leptospirose é uma doença sistêmica causada por bactéria Leptospira spp. que afeta seres humanos, animais domésticos e selvagens. O presente trabalho investigou a presença de anticorpos anti-Leptospira spp. na população canina da cidade de Teresina-Piauí, e os respectivos sorovares predominantes. Amostras de sangue de 425 cães foram coletadas no Centro de Controle de Zoonoses de Teresina, no período de julho de 2010 a janeiro de 2012, e submetidas à prova de Soroaglutinação Microscópica (SAM). Este estudo encontrou uma taxa de infecção média com 17,41% (IC 95%; 13,8 – 21,0) e 11 sorovares reagentes, sendo os prevalentes Canicola (18,9%), Autumnalis (16,2%), Icterohaemorrhagiae (12,1%) e Butembo (12,1%). As questões levantadas neste estudo indicam a ocorrência de infecção por Leptospira spp em cães da cidade de Teresina-Piauí, Brasil.

Leptospirosis is a worldwide zoonosis whose transmission is interlinked by multiple factors in the man-animal-ecosystem interface. This study aimed to evaluate the risk factors for the occurrence of anti-Leptospira antibodies in dogs in the capital Teresina (PI), and to determine their spatial distribution. Five hundred fifty-eight dog blood samples were submitted to the Microscopic Serum Agglutination (MSA) test. We applied semi-structured questionnaires to dog owners and obtained the area of residence for projection in geographical maps. Serum prevalence was 13.8%, in which the most common serovar was icterohaemorrhagiae, with 49.2%. Dogs with street access, failure to collect food bowl and low income of owners were risk factors. There was a higher number of seropositive dogs in the rainy season, with 87.1%, which is a probable risk factor for the occurrence of cases. The distribution of seropositive dogs was widely spread in the city, with predominance of cases in anthropized areas. These risk factors favor the occurrence of anti-Leptospira antibodies in dogs that are agent maintenance sources in the city and reinforce the need for epidemiological and environmental surveillance to prevent leptospirosis.

In vitro senescence of multipotent cells has been commonly associated with DNA damage induced by oxidative stress. These changes may vary according to the sources of production and the studied lineages, which raises questions about the effect of growing time on genetic stability. This study is aimed at evaluating the evolution of genetic stability, viability, and oxidative stress of bone marrow mesenchymal stem cells (MSCBMsu) and renal progenitor cells of the renal cortex (RPCsu) of swine (Sus scrofa domesticus) in culture passages. P2, P5, and P9 were used for MSCBMsu and P1, P2, and P3 for RPCsu obtained by thawing. The experimental groups were submitted to MTT, apoptosis and necrosis assays, comet test, and reactive substance measurements of thiobarbituric acid (TBARS), nitrite, reduced glutathione (GSH), and catalase. The MTT test curve showed a mean viability of 1.14±0.62 and 1.12±0.54, respectively, for MSCBMsu and RPCsu. The percentages of MSCBMsu and RPCsu were presented, respectively, for apoptosis, an irregular and descending behavior, and necrosis, ascending and irregular. The DNA damage index showed higher intensity among the MSCBMsu in the P5 and P9 passages (p<0.05). In the TBARS evaluation, there was variation among the lines of RPCsu and MSCBMsu, presenting the last most significant variations (p<0.05). In the nitrite values, we identified only among the lines, in the passages P1 and P2, with the highest averages displayed by the MSCBMsu lineage (p<0.05). The measurement of antioxidant system activity showed high standards, identifying differences only for GSH values, in the RPCsu lineage, in P3 (p<0.05). This study suggests that the maintenance of cell culture in the long term induces lower regulation of oxidative stress, and RPCsu presents higher genetic stability and lower oxidative stress than MSCBMsu during in vitro expansion.

uma terapia alternativa/coadjuvante para a recuperação do parênquima renal. Este estudo possibilitou identificar as principais áreas de depósito de pesquisas, assim como o crescente interesse de inovações nessa área.

A B S T R A C TThis research aimed to evaluate the topography, external morphology, the intraparenchymal branching and distribution of the linens arterial vessels of the spleen of the cutia (Dasyprocta prymnolopha). Twenty spleens were analyzed of young and adult animals, thirteen males and seven females, from the Center for the Study and Preservation of Wild Animals of the Agricultural Sciences Center of the UFPI. In sixteen spleens, the topography and the external morphology were verified "in situ" after the opening of the abdominal cavity. For the study of arterial vascularization, ten organs were used, six of them were removed from the abdominal cavity, and injected with 60% latex Neoprene through the linens artery, fixed with 10% aqueous solution of formaldehyde, and dissected. In four other spleens, the artery was filled with a solution of colored vinyl acetate, submitted to the corrosion method, using 30% sulfuric acid, obtaining the vascular molds. The spleen of the cutia with triangular shape was verified in 68.7%, and in 31.3%, rectangular. The linens artery, always with extra-parenchymal division, originates from 5 to 12 branches, predominantly 6 branches, in 40% of cases, that penetrate the parenchyma, vascularize independent territories, characterizing the arterial segments. In 20% of the spleens examined, we observed extrahilar arteries and, in only 1 case (10%) an anastomosis was seen between the segmental arterial branches. K E Y W O R D SAnatomy, Splenic artery, Wild animals R E S U M OEsta pesquisa teve por objetivo avaliar a topografia e morfologia externa, ramificação e distribuição intraparenquimal dos vasos arteriais lienais do baço de cutia (Dasyprocta prymnolopha). Foram analisados vinte baços de animais jovens e adultos, treze machos e sete fêmeas, procedentes do Núcleo de Estudos e Preservação de Animais Silvestres do Centro de Ciências Agrárias da UFPI. Em dezesseis baços, verificou-se "in situ" a topografia e a morfologia externa, após a abertura da cavidade abdominal. Para o estudo da vascularização arterial, foram utilizados dez órgãos, destes, seis foram retirados da cavidade abdominal, injetados com Neoprene látex a 60%, por meio da artéria lienal, fixados com solução aquosa de formaldeído a 10%, e dissecados. Em outros quatro baços, procederam a repleção da referida artéria, com solução de acetato de vinil corado, submetidos ao método de corrosão, utilizando-se ácido sulfúrico a 30%, obtendo-se os moldes vasculares. Verificou-se o baço da cutia com formato triangular em 68,7%, e em 31,3%, retangular. A artéria lienal, sempre com divisão extra-parenquimal origina de 5 a 12 ramos, preponderantemente, 6 ramos, em 40% dos casos, que penetram no parênquima, vascularizam territórios independentes, caracterizando os segmentos arteriais. Em 20% dos baços examinados, observou-se artérias extra-hilares e, em apenas 1 caso (10%) foi visto uma anastomose entre os ramos arteriais segmentares.

/agrariacad Efeitos biológicos da radiação e radioproteção-uma revisão sistemática. Biological effects of radiation and radioprotection-a systematic review.

The agouti has been used as an experimental model in several studies focused on reproductive biology. The umbilical cord, an embryonic attachment that connects the foetus to the placenta, has been reported as an important anatomical site for obtaining stem cells. The objective of this study was to describe macro- and microscopically the umbilical cord of agoutis at different stages of gestation, to expand and cultivate in vitro the progenitor cells and to report their morphological characteristics. Seven cutias were submitted to caesarean section to collect the umbilical cords: five were destined for studies of cord structure in different stages of gestation (30, 35, 50, 75 and 100 days postcoital), and two were collected in the third stage of gestation for isolation and cell culture. The umbilical cord of cutias assumes a spiral arrangement, with veins and arteries on it starting 50 days after coitus. The arteries present an outer layer of smooth muscle fibres in a longitudinal and circular arrangement and a medium layer of smooth muscle fibres with only longitudinal and intimate orientation and coated by the endothelium. The veins consist of longitudinal smooth muscle fibres with an extract of smooth muscle cells, and the endothelium, in all analysed gestational phases, is a structure bounded by simple pavement epithelial tissue originating from the amnion, adhered to Wharton's Jelly and forming the umbilical vessels and allantoid duct. The proposed protocol allowed the collection of a high cellular concentration of umbilical cord progenitor cells from viable cutias.

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