Oral fibrosarcoma in jararaca (Bothrops pubescens): anatomopathological and immunohistochemical aspects

Abstract

RESUMO.-[Fibrossarcoma oral em jararaca (Bothrops pubescens): aspectos anatomopatológicos e imuno-histoquímicos.] Uma serpente de cativeiro, fêmea, quatro anos de idade, do gênero Bothrops apresentou aumento de volume no lado esquerdo da cavidade oral, sugerindo tratar--se de neoplasma. A massa foi removida cirurgicamente e enviada para exame anatomopatológico. Dois meses depois foi observado novo aumento de volume no mesmo local, sugerindo recidiva. A lesão foi removida por completo e também enviada para análise. Histologicamente, as duas amostras consistiam de massa altamente celular, composta por células anaplásicas fusiformes organizadas em feixes entrelaçados e distribuídos por toda extensão tecidual e, ocasionalmente, células poligonais arranjadas em fascículos irregulares. A coloração de tricrômico de Masson apresentou quantidade modesta de colágeno sustentando as células neoplásicas. Não foi observado conteúdo PAS-positivo no citoplasma das células neoplásicas. Os achados histológicos e histoquímicos indicavam tratar-se de neoplasma de célu-las fusiformes, porém não era possível sua classificação. A E-mail: acmotta@upf.br A 4-year-old female captive-bred snake of the genus Bothrops showed swelling on the left side of the oral cavity, suggesting the development of neoplasia. The mass was removed surgically and sent for pathological examination. Two months later a new increase in volume in the same site was observed, suggesting recurrence. The lesion was completely removed and sent for pathological analysis. Histologically, the two-samples consisted of a mass with highly-cell density composed of spindle-shaped anaplastic cells arranged in interwoven bundles, distributed throughout the tissue extension and, occasionally, polygonal cells arranged in irregular fascicles. The Masson trichrome staining showed modest amount of collagen supporting the neoplastic cells. PAS-positive content was not observed in the cytoplasm of neoplastic cells. Histological and histochemical findings indicated that it was a spindle cell neoplasm, but the classification was not possible. Immunohistochemistry was requested and performed using the streptavidin-biotin-peroxidase method. The markers used were anti-vimentin, anti-PCNA, anti-EMA, anti-melan A and anti-melanosome, anti--desmin, anti-actin, anti-CD68 and anti-S100protein. The neoplastic cells were immunoreactive for vimentin and PCNA and negative for the other antibodies. The morphology characterization, histochemical and immunohistochemical analysis of neoplastic cells allowed the definitive diagnosis of oral fibrosarcoma. Oral fibrosarcoma in jararaca (Bothrops pubescens): anatomopathological and immunohistochemical aspects imuno-histoquímica foi requisitada e realizada pelo método streptavidina-biotina-peroxidase, utilizando os anticorpos anti-vimentina, anti-PCNA, Anti-EMA, anti-melan A, anti--HMB45, anti-desmina, anti-actina, anti-CD68 e anti-proteí-na S-100. As células neoplásicas foram imunorreativas para vimentina e PCNA e, negativas para os demais anticorpos. A ca...

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“…Second, as an antihuman antibody was used in this study, the result could have been false-negative [22]. According to previous studies, the negative results could be due to the non-specificity of the antibody [22][23][24][25][26]. Moreover, the positive controls, smooth and striated muscle samples, in this case have demonstrated to be negative to vimentin, meaning non-specificity of the antibody in this reptile species (Supplementary Figure S1).…”

“…Therefore, although the tumor originated from smooth muscle cells, it was possible to lack reactivity to vimentin. Second, as an antihuman antibody was used in this study, the result could have been false-negative [22]. According to previous studies, the negative results could be due to the non-specificity of the antibody [22][23][24][25][26].…”