Avaliação microbiológica, histológica e imunológica de frangos de corte desafiados com Salmonella Enteritidis e Minnesota e tratados com ácidos orgânicos

Pickler, Larissa; Hayashi, R. M.; Lourenço, Miguel L.; Miglino, Leonardo B.; Caron, Luíz Felipe; Beirão, Breno Castello Branco; Silva, Ana V.F.; Santín, Elizabeth

doi:10.1590/s0100-736x2012000100006

Cited by 32 publications

(26 citation statements)

References 24 publications

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“…The Ecology and Animal Epidemiology session of the 13 th International Symposium Salmonella and Salmonellosis, held in France in 2013, concluded that wild birds and reptiles may be considered as new epidemiological routes of Salmonella (International Symposium Salmonella and Salmonellosis, 2103). Pickler et al 2012 evaluated the effectiveness of the supplementation of broilers with organic acids in the feed and drinking water during the pre-harvest period for the prevention of Salmonella Minnesota and Salmonella Enteritidis contamination, and showed that organic acids were not effective against Salmonella Minnesota. The use of organic acid during the preharvest period is recommended by international guidelines (CODEX, 2011;OIE, 2015), and it is adopted by many Brazilian poultry companies.…”

Section: Resultsmentioning

confidence: 99%

Serotyping and Genotyping of Salmonella Strains Isolated From Broilers, Chicken Carcasses Before and After Chilling, and Frozen Chicken Breasts Produced in The States of Mato Grosso do Sul and Santa Catarina, Brazil

Machado

Pereira

Aquino

et al. 2017

Rev. Bras. Cienc. Avic.

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The present study investigated the effectiveness of a single Salmonella prevention and control program applied in two different processing plants, located in the states of Mato Grosso do Sul (plant A) and Santa Catarina (Plant B), belonging to the same company, and identified Salmonella strain subtypes isolated from broilers, carcasses before and after chilling, and frozen chicken breasts. The Salmonella prevention and control program was 90% effective in plant A and 100% in plant B, considering a level of 10% positive samples per frozen chicken breast batch acceptable. A total of 128 strains were serotyped, being 10 from drag swabs, 31 from cloacal swabs, 83 from carcasses, and 4 from frozen chicken breasts. After serotyping analysis, 30 strains isolated at different processing steps and drag swabs, and three Salmonella Minnesota strains isolated in 2012 in plant A, were genotyped by PFGE. In plant A, the most frequently strain isolated was Salmonella Minnesota (90.35%), followed by Salmonella Newport (8.77%), and in Plant B, Salmonella Senftenberg (80%). Salmonella Minnesota strains were differentiated by PFGE into 19 pulsotypes distributed in three clusters. The phenotypic identification by serotyping of four strains diverged from their PFGE genotypic results. Most Salmonella Minnesota strains genotyped in plant A and the strains isolated from environmental samples in 2012 in the same broiler processing plant belong to a single cluster, confirming the dominance and persistence of this clone over time.

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Section: Resultsmentioning

confidence: 99%

Serotyping and Genotyping of Salmonella Strains Isolated From Broilers, Chicken Carcasses Before and After Chilling, and Frozen Chicken Breasts Produced in The States of Mato Grosso do Sul and Santa Catarina, Brazil

Machado

Pereira

Aquino

et al. 2017

Rev. Bras. Cienc. Avic.

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“…This sample was rendered resistant to antibiotics (nalidixic acid and novobiocin), which were incorporated into brilliant green agar to inhibit gut microbiota and facilitate counting. SH was grown in BHI broth for 18-24 h at 37°C until reaching a concentration of 1.0 × 10 9 CFU/ml; serial dilution was then performed until a concentration of 1.0 × 10 6 CFU/ml was achieved (Pickler et al, 2012). For birds in groups 1 and 2, 0.5 ml of this culture was inoculated orally at 3 Note: T1 (vaccinated and challenged at 3 days of age), T2 (unvaccinated and challenged at 3 days of age), T3 (vaccinated and challenged at 21 days of age), T4 (unvaccinated and challenged at 21 days of age).…”

Section: Challengementioning

confidence: 99%

Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with Salmonella Heidelberg

Muñíz¹,

Verdi²,

Leão³

et al. 2017

Avian Pathology

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Salmonellosis ranks among the major diseases of commercial poultry, and its presence in poultry flocks is responsible for economic losses and risks related to public health. Vaccines are an important tool within integrated programmes to control salmonellosis. The purpose of this study was to assess cross-protection provided by the Poulvac® ST vaccine in the control of Salmonella Heidelberg in experimentally challenged 3-and 21-day-old birds. Eighty birds were identified and separated into four treatments (T1: vaccinated and challenged at 3 days of age, T2: unvaccinated and challenged at 3 days of age, T3: vaccinated and challenged at 21 days of age, and T4: unvaccinated and challenged at 21 days of age). The inoculum was produced from a Brazilian field strain of SH. At the end of the experiment, caecum and liver/ spleen samples were collected for quantitative and qualitative analysis of SH, respectively. Analysis of the liver/spleen showed that Poulvac® ST significantly (P ≤ 0.05) reduced the percentage of SH positivity in the group challenged at 3 days of age, while in the group challenged at 21 days this difference was almost considered significant (P = 0.1818). On the other hand, there was no statistically significant difference in SH count in the caecum (CFU/g) in the group challenged at 3 days, but for the group challenged at 21 days the SH counts were significantly (P ≤ 0.05) lower in the vaccinated group when compared to the positive control.ARTICLE HISTORY

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“…O líquido foi diluído até a concentração 0,5 da escala de MacFarland, que corresponde a concentração 10 8 de UFC (Unidades Formadoras de Colônias). Fez-se diluições seriadas em tubos contendo 9 mL de solução fisiológica esterilizada até a concentração 10 6 UFC/mL sendo posteriormente cultivada em ágar Mueller Hinton para determinar a contagem de unidades formadoras de colônias por mL (Pickler et al, 2012),…”

Section: Digestibilidadeunclassified

“…As análises de contagem de Salmonella foram realizadas de acordo com Pickler et al (2012). Os suabes de cloaca, os inglúvios inteiros e os cecos com conteúdo foram macerados e diluídos em água peptonada a 2% em proporção de 1:10.…”

Section: Análises Microbiológica Histopatológica E Imuno-histoquímicaunclassified

Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella

et al. 2015

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RESUMO.-Objetivou-se com o presente estudo comparar o efeito de diferentes sorovares de Salmonella na resposta imune local da mucosa do intestino de frangos de corte. Aos sete dias de idade, as aves foram desafiadas com os sorovares S. Enteritidis, S. Typhimurium, S. Senftenberg, S. Mbandaka e S. Minnesota. Foi observado que todos os sorovares testados foram capazes de colonizar o intestino das aves sendo possível o isolamento de Salmonella em suabes de cloaca, 48 h após inoculação. De maneira geral, as aves do grupo controle negativo, que não foram desafiados apresentaram quantidade significativamente menor de células imunológicas na mucosa intestinal do que as aves desafiadas. Porém, verificou--se que os sorovares de Salmonella, utilizados neste estudo, apresentaram diferentes efeitos sobre a dinâmica celular da mucosa do íleo e ceco e afetaram de modo diferente o ganho de peso e ganho médio diário das aves demonstrando distintos graus de patogenicidade. Os sorovares Enteritidis e Typhimurium apresentaram um efeito mais intenso tanto no desempenho quanto na mobilização de células imunoló-gicas na mucosa intestinal de frangos de corte. The study was designed to compare the effect of different Salmonella serovars in immune response across the count of CD8+ cells, CD4+ cells, goblet cells and macrophages in the gut mucosa of broilers. During the experimental inoculation at 7 day-old were used Salmonella enterica subspecies enterica sorovars Enteritidis, Typhimurium, Senftenberg, Mbandaka and Minnesota. It was observed that all serovars tested were capable of contaminating the poultry being possible counts of Salmonella in cloacal swabs, 48 h after inoculation and into the crop and cecum, at 14 and 20 day-old. Serovars tested had different effects on broiler performance assessed at 20 days. In the mucosa of the ileum and cecum of broilers, it was observed that some of the serotypes increased CD8 + cells, CD4 + cells, goblet cells and macrophages compared to the negative control group both at 14 and at 20 day-old. S. Enteritidis and S. Typhimurium are the serovars that showed the more intense effect in live performance and in the immune system of birds showing pathogenic characteristic; generally the broilers of the negative control showed significantly less immune cells on the intestinal mucosa than broilers inoculated experimentally. However, it was found that the Salmonella serovars used in this study had different effects on the cellular dynamics of the mucosa of the ileum and cecum and differently affect weight gain and average daily gain of poultry showing different levels of pathogenicity. Avaliação da resposta imunológica da mucosa intestinal

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Avaliação microbiológica, histológica e imunológica de frangos de corte desafiados com Salmonella Enteritidis e Minnesota e tratados com ácidos orgânicos

Cited by 32 publications

References 24 publications

Serotyping and Genotyping of Salmonella Strains Isolated From Broilers, Chicken Carcasses Before and After Chilling, and Frozen Chicken Breasts Produced in The States of Mato Grosso do Sul and Santa Catarina, Brazil

Serotyping and Genotyping of Salmonella Strains Isolated From Broilers, Chicken Carcasses Before and After Chilling, and Frozen Chicken Breasts Produced in The States of Mato Grosso do Sul and Santa Catarina, Brazil

Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with Salmonella Heidelberg

Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella

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