Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with <i>Salmonella</i> Heidelberg

Muñíz, Eduardo; Verdi, R.; Leão, Joice Aparecida; Back, Alberto; Nascimento, Vladimir Pinheiro do

doi:10.1080/03079457.2017.1348598

Cited by 26 publications

(14 citation statements)

References 35 publications

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“…Based on these findings, we hypothesized that 24 hours after the booster vaccine was administered via drinking water, the immune cells continuously migrate to the liver and maintain a systemic and specific immune response, even though Salmonella recovery was not statistically reduced in this organ at 28 days. Muniz et al (2017), in a study of the same live ST vaccine administered via spray on the first day, observed reduced SH recovery in the ceca of 21-day-old broilers, results similar to those obtained herein.…”

Section: Discussionsupporting

confidence: 89%

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Salmonella Typhimurium Vaccine to Control a Brazilian Salmonella Heidelberg Strain in Broiler Chickens

Hayashi¹,

Tujimoto-Silva²,

Muñíz³

et al. 2018

Ars Vet

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Foodborne Salmonella infections in humans, which results from the consumption of contaminated poultry meat and eggs, are a major public health concern. Vaccination of animals against Salmonella is one strategy to prevent these infections and reduce the risks to public health. Live attenuated Salmonella enterica vaccines can confer protection against salmonellosis by inducing both cell-mediated and mucosal immune responses. This study assessed a live, attenuated Salmonella enterica Typhimurium (ST) vaccine in broiler chickens against a heterologous challenge with Salmonella Heidelberg (SH) by evaluating bacterial quantification, immune cells infiltration, and cytokine gene expression in the cecum. The treatments were: T1, non-vaccinated, non-challenged; T2, non-vaccinated, SH-challenged; T3, ST-vaccinated and SH-challenged. At 28 days of age, the ST-vaccinated group had significantly recovered reduction of SH in the crop (P<0,01) and cecum (P = 0,021) compared to the non-vaccinated SH-challenged group, with no significant changes (P˃0,05) in macrophages, T CD4+, or T CD8+ cells dynamics during the same period. Aerosol vaccination on the first day promoted greater interleukin-12 expression in the liver (P<0,05) and interleukin-10 expression and T CD8+ cells in the ileum 16 hours after housing. After prime-boosted oral immunization on the 13th day, the vaccinated group had greater expression of macrophages and T CD4+ cells in the liver (P<0,05) than the control group. Two doses of a live ST-attenuated vaccine promoted a partial cross-protective effect against SH strain UFPR1 challenge in broilers.

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Section: Discussionsupporting

confidence: 89%

“…enterica serovar Typhimurium (STM-1), this serovar contains immunological antigens which are the same as those seen in other group B Salmonellae (such as SH). This similarity in the somatic and flagellar antigens explains the good heterologous immune response (MUNIZ et al, 2017).…”

Section: Discussionmentioning

confidence: 92%

Salmonella Typhimurium Vaccine to Control a Brazilian Salmonella Heidelberg Strain in Broiler Chickens

Hayashi¹,

Tujimoto-Silva²,

Muñíz³

et al. 2018

Ars Vet

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“…While investigating the contribution of intestinal microbiota on the vaccine response is of growing interest, it is also likely there is an effect of vaccination on the gut microbiota in chicken. A few recent studies report the effect of speci c vaccination against Salmonella on their gut microbiota (27).…”

Section: Introductionmentioning

confidence: 99%

Caecal Microbiota Composition of Experimental Laying Hens Differs According to Genetic Line and Vaccination to IBV

Borey

Bed'Hom

Bruneau

et al. 2021

Preprint

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BackgroundInteractions between the gut microbiota and the immune system may be involved in the vaccine response. In the present study, we studied the correlations between caecal microbiota composition and the immune response in six experimental laying hen lines harboring different haplotypes at the Major Histocompatibility complex (MHC), 7 weeks after their first vaccination against the infectious bronchitis virus (IBV). Two lines were previously considered as high responders (HR) to IBV vaccination and two other ones as low responders (LR). We explored to what extent the gut microbiota could be related to this variability through the characterization of caecal bacterial communities with a 16S rRNA gene amplicon sequencing approach, one week after an IBV infectious challenge. ResultsWe observed significant effects of both the vaccination and the genetic line on the microbiota, with lower bacterial richness in vaccinated chickens, especially in the Ruminococcaceae family. We also observed dissimilar caecal communities among the different MHC lines, and among the vaccinated and non-vaccinated animals. The effect of vaccination was similar in all the lines, with a reduced abundance of OTUs from the Ruminococcacea UCG-014 and Faecalibacterium genera, and an increased abundance of OTUs from the Eisenbergiella genus. The main association between the microbiota and the immune phenotypes implied TCRϒδ expression on TCRϒδ+ T cells, which especially shared negative associations with OTUs from the Escherichia-Shigella genus. Finally, opposing the HR and LR groups, we identified 3 OTUs more abundant in the HR group, from the Tyzzeralla family, the Angelakisella genus, and to a lower extent the Flavonifractor family. Conversely, an OTU from the CAG-352 genus was more abundant in the LR group. ConclusionsWe assessed that the caecal microbiota composition is associated with the IBV vaccine response in laying chickens, and that TCRϒδ expression on TCRϒδ+ T cells appears as one of the main components potentially involved in this interaction.

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“…Live Salmonella vaccines are commonly used to reduce colonization of broad-host Salmonella enterica serovars in poultry (Hassan and Curtiss, 1997), the primary vehicle of human salmonellosis (Batz et al, 2012). These vaccines successfully reduce Salmonella ceca colonization (Muniz et al, 2017) and environmental contamination (Dórea et al, 2010). Given that S. enterica serovar Typhimurium virulence is stimulated by norepinephrine and epinephrine (Karavolos et al, 2008;Pullinger et al, 2010), S. Typhimurium-derived vaccines could also respond to neurochemicals.…”

Section: Introductionmentioning

confidence: 99%

Oral Treatments With Probiotics and Live Salmonella Vaccine Induce Unique Changes in Gut Neurochemicals and Microbiome in Chickens

et al. 2020

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Cross-talk between the gut microbiota and neurochemicals affects health and wellbeing of animals. However, little is known about this interaction in chickens despite their importance in food production. Probiotics and live Salmonella vaccines are microbial products commonly given orally to layer pullets to improve health and ensure food safety. This study's objective was to determine how these oral treatments, individually or in combination, would impact the gut environment of chickens. White Leghorn chicks were either non-treated (CON) or orally given probiotics (PRO), a recombinant attenuated Salmonella vaccine (RASV; VAX), or both (P+V). Birds were fed with probiotics daily beginning at 1-day-old and orally immunized with RASV at 4-days-old and boosted 2 weeks post-primary vaccination. At 5 weeks, ceca content, ceca tissues, and small intestinal scrapings (SISs) were collected from ten birds/group post-euthanasia for analyses. Catecholamine, but not serotonergic, metabolism was affected by treatments. Dopamine metabolism, indicated by L-DOPA and DOPAC levels, were increased in P+V birds versus CON and PRO birds. Based on 16S sequencing, beta diversity was more similar among vaccinated birds versus birds given probiotics, suggesting live Salmonella vaccination has a major selective pressure on microbial diversity. Abundances of Akkermansia muciniphila and Enterobacteriaceae positively correlated with levels of tyrosine and norepinephrine, respectively. Both enumeration and 16S sequencing, determined that PRO exhibited the greatest levels of Enterobacteriaceae in the ceca and feces, which was associated with greater IgA production against E. coli virulence factors as tested by ELISA. In summary, we demonstrate that using probiotics alone versus in combination with a live vaccine has major implications in catecholamine production and the microbiota of layer pullets. Additionally, unique correlations between changes in some neurochemicals and specific bacteria have been shown.

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Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with Salmonella Heidelberg

Cited by 26 publications

References 35 publications

Salmonella Typhimurium Vaccine to Control a Brazilian Salmonella Heidelberg Strain in Broiler Chickens

Salmonella Typhimurium Vaccine to Control a Brazilian Salmonella Heidelberg Strain in Broiler Chickens

Caecal Microbiota Composition of Experimental Laying Hens Differs According to Genetic Line and Vaccination to IBV

Oral Treatments With Probiotics and Live Salmonella Vaccine Induce Unique Changes in Gut Neurochemicals and Microbiome in Chickens

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