Primary metabolite mobilization during germination in rosewood (Aniba rosaeodora Ducke) seeds

Lima, Renata Braga Souza; Gonçalves, José Francisco de Carvalho; Pando, Silvana Cristina; Fernandes, Andréia Varmes; Santos, André Luis Wendt dos

doi:10.1590/s0100-67622008000100003

Cited by 22 publications

(15 citation statements)

References 24 publications

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“…In a study on reserve mobilization in Aniba rosaeodora seeds, there was a 30% reduction in soluble sugars when the radicle length was 2-5 cm, with this mobilization being associated with the formation of the first leaf (Lima et al, 2008). Soluble sugar levels also decreased during imbibition of Senna macranthera , Apuleia leiocarpa (Pontes et al, 2002) and Caesalpinia peltophoroides (Corte et al, 2006) seeds, indicating the mobilization of carbohydrates for the germination process.…”

Section: Resultsmentioning

confidence: 99%

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

et al. 2013

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Seed imbibitions is the first stage of the germination process and is characterized by the hydration of tissues and cells and the activation and/or induction of the enzymes responsible for mobilizing reserves for respiration and the construction of new cell structures. The objective of this study was to investigate the alterations in reserve substances during slow hydration of Bahia Rosewood (Dalbergia nigra) seeds in water. Seeds from two different lots (Lot I and II) were placed in saturated desiccators (95-99% RH) to hydrate at 15 and 25 °C until water contents of 10, 15, 20 and 25% were reached. At each level of hydration, changes in lipid reserves, soluble carbohydrates, starch and soluble proteins were evaluated. The mobilization of reserves was similarly assessed in both lots, with no differences being observed between the two hydration temperatures. Lipid contents showed little variation during hydration, while the contents of soluble carbohydrates and starch decreased after the 15% water content level. Soluble proteins showed a gradual tendency to decrease between the control (dry seeds) up to 25% water content.

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Section: Resultsmentioning

confidence: 99%

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

et al. 2013

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“…Durante a germinação de sementes de Aniba rosaeodora, o decréscimo no teor de amido foi relacionado ao aumento na atividade da α-amilase, indicando ser esta enzima a responsável pela quebra inicial do amido na espécie (Lima et al, 2008).…”

Section: Introductionunclassified

Alterações nas reservas de sementes de Melanoxylon brauna Schott. (Fabaceae Caesalpinoideae) durante a germinação em diferentes temperaturas

Ataíde

Borges

Picoli

et al. 2017

Agraria

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RESUMODurante a germinação, as sementes utilizam os compostos de reserva para fornecerem os nutrientes necessários até que as plântulas se tornem autotróficas. Neste trabalho objetivou-se investigar as alterações nas reservas de carboidratos, amido, lipídios e proteínas em sementes de Melanoxylon brauna durante o período germinativo em diferentes temperaturas. Para tanto, sementes da espécie foram colocadas para germinar sob temperaturas de 10, 25, 30 e 40 °C, sendo retiradas amostras de sementes a cada 24 horas durante o período de 96 horas. Uma pequena redução nos teores de lipídios foi observada durante a germinação nas temperaturas analisadas, enquanto os teores de açúcares solúveis decresceram a partir de 48 horas de embebição, sendo prontamente utilizados, especialmente nas temperaturas de 25 e 30 °C. Para as proteínas solúveis, verificou-se declínio desde o início do período germinativo às temperaturas de 25 e 30 °C, sendo este mais intenso no período pós-germinativo à temperatura de 30 °C. Conclui-se que as reservas de carboidratos solúveis, lipídios e proteínas decresceram durante o período germinativo de sementes de Melanoxylon brauna, variando esses decréscimos de acordo com a temperatura. Palavras-chave: amido; carboidratos; lipídios; proteínas Changes in seed reserves of Melanoxylon brauna Schott. (Fabaceae Caesalpinoideae) during germination at different temperatures ABSTRACTDuring germination, seeds use the reserve compounds to provide the nutrients until the seedlings become autotrophic. The aim of this study was to investigate the changes in carbohydrates, starch, lipids and proteins reserves during germination of Melanoxylon brauna seeds at different temperatures. For both, the seeds were germinated at temperatures of 10, 25, 30 and 40 °C, and samples were collected every 24 hours during 96 hours. Was observed small reduction of lipid levels during germination in temperatures analyzed, while the levels of soluble sugars decreased from 48 hours of soaking, being readily used, especially at temperatures of 25 and 30 °C. For soluble proteins, there was a decrease from the beginning of the germination period at temperatures of 25 to 30 °C, this being more intense in the post-germination at 30 °C period. It is concluded that soluble carbohydrate, lipid and protein reserves decreased during the germination period of Melanoxylon brauna seeds, varying these decreases according to temperature.

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“…progressive increases in dry matter mass due to the synthesis and deposition of reserves, such as carbohydrates, lipids and proteins (Suda & Giorgini, 2000;Lima et al, 2008;Bewley et al, 2013). These processes occur in specific organelles that may be present in the embryo and/or reserve tissues, such as the endosperm.…”

Section: Introductionmentioning

confidence: 99%

“…Proteins store mainly nitrogen and sulfur, which are essential for the synthesis of new proteins, nucleic acids, and secondary compounds in the growing seedling (Lima et al, 2008;Bewley et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Histochemical changes during the ontogeny of malagueta and biquinho pepper seeds

Abud

Araújo

et al. 2017

Acta Sci. Agron.

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ABSTRACT. This study aimed to evaluate the structural changes and reserve deposition of malagueta and biquinho pepper seeds and to determine their chemical composition during ontogeny. The fruits were collected 25, 40, 55, 70, 85, and 100 days after anthesis (DAA). After collection, the seeds were extracted and fixed in FAA 50 and then stored in 70% ethanol. Subsequently, the seeds were embedded in methacrylate (Historesin) and sectioned with a microtome. The sections were stained with Toluidine blue O for anatomical analysis and Xylidine Ponceau, Periodic acid Schiff (PAS) and Lugol's iodine solution for histochemical analysis. Sections were made using a cryomicrotome for acid phloroglucinol staining and Sudan III tests. Progressive lignification of the seed coat cells was observed. Proteins and lipids were the main reserve compounds of malagueta and biquinho pepper seeds stored in the embryo and endosperm. Starch served as a transient reserve during embryo development. The lipid reserves were observed in embryo and endosperm cells from 25 until 100 DAA. At 100 DAA, the protein contents of the malagueta and biquinho pepper seeds were 0.33 and 0.62 g g Alterações histoquímicas durante a ontogenia de sementes de pimenta malagueta e biquinho RESUMO. Objetivou-se acompanhar as alterações estruturais, a deposição de reservas e determinar a composição química de sementes de pimenta malagueta e biquinho durante a sua ontogênese. Frutos foram coletados aos 25, 40, 55, 70, 85 e 100 dias após a antese (DAA). Após a coleta, as sementes foram extraídas e fixadas em FAA 50 e, armazenadas em álcool 70%. Posteriormente, foram incluídas em Historesina Metacrilato e seccionadas em micrótomo. Os cortes foram submetidos à coloração com Azul de Toluidina, para análise anatômica e a análise histoquímica realizada com XylidinePonceau, Periodic acid Schiff (PAS) e Lugol. Para reação com Floroglucina Ácida e Sudam III, foram realizados cortes em criomicrótomo. Verificou-se que houve lignificação progressiva das células do tegumento. As proteínas e os lipídeos constituem os principais compostos de reserva das sementes de pimenta malagueta e biquinho, estocados no embrião e endosperma. O amido constitui uma fonte transitória de reserva para o desenvolvimento do embrião. As reservas lipídicas são observadas nas células do embrião e do endosperma dos 25 aos 100 DAA. Aos 100 DAA, as sementes de pimenta malagueta e biquinho apresentam concentração de proteínas de 0,33 e 0,62 g g -1 M.S. e lipídeos de 0,30 e 0,25 g g -1 M.S. respectivamente.

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Primary metabolite mobilization during germination in rosewood (Aniba rosaeodora Ducke) seeds

Cited by 22 publications

References 24 publications

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

Alterações nas reservas de sementes de Melanoxylon brauna Schott. (Fabaceae Caesalpinoideae) durante a germinação em diferentes temperaturas

<b>Histochemical changes during the ontogeny of <i>malagueta</i> and <i>biquinho</i> pepper seeds

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