Comportamento fisiológico de sementes osmocondicionadas de Platymiscium pubescens Micheli (tamboril-da-mata)

e, Eduardo Borges Euclydes de Lima; J, De; Gonçalves, Rita de Cássia; de, Sebastião Rezende Tavares; Ribeiro, Silvana Garcia

doi:10.1590/s0100-67622002000500011

Cited by 10 publications

(14 citation statements)

References 17 publications

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“…It was not detected in the embryonic axis, which reinforces the possibility of its use. Another possibility is that it may have been eliminated into the medium during imbibition, similarly to what was observed by Borges et al (2002) in Platymiscium pubescens seeds, when a significant reduction was noticed in rhamnose, xylose and galactose contents. Average mannose contents increased significantly in cotyledons during imbibition in Apuleia leiocarpa seeds, denoting variations in behavior depending on the species (PONTES et al 2002).…”

Section: Carrijo L C Et Alsupporting

confidence: 53%

“…According to Feurtado et al (2001), -galactosidase was detected in the embryo and also in lateral and micropylar portions of the endosperm in Lycopersicon esculentum seeds. Borges et al (2002), on the other hand, found no relationship between -galactosidase activity and changes in the composition of the cell wall or in sugar contents in Platimiscium pubescens seeds during germination. After its characterization in cotyledons and in the embryonic axis of dormant Caesalpinia peltophoroides seeds, Borges et al (2005) concluded that it is present in different isoforms within seed structures.…”

Section: Carrijo L C Et Almentioning

confidence: 79%

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α-galactosidase activity and carbohydrate mobilization in seeds of Dalbergia nigra (vell.) Allemão ex Benth. - fabaceae (Brazilian rosewood) during germination

Carrijo

Borges

Pontes

et al. 2010

CERNE

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Complex substances are converted by enzyme action into soluble molecules during the germination process, and these are in turn translocated to the growing plant embryo, serving as an energy source or physical structure. With the objective of quantifying -galactosidase enzyme activity as well as mono-and oligosaccharide mobilization during germination, this study was conducted using Brazilian rosewood seeds. Seeds were kept in a germinator at 25ºC under continuous light for evaluation of radicle emergence (protrusion) over a period of 10 days. Mono-and oligosaccharide contents and also the specific activity of -galactosidase enzyme were quantified on days zero, one, three and five. There was mobilization of glucose and mannose stored in cotyledons, and also of xylose in cotyledons and in the embryo in the first three days of germination. Rhamnose contents increased in cotyledons and in the embryonic axis. Raffinose was the initially used oligosaccharide, both in the embryonic axis and in cotyledons, while sucrose accumulated in both. Enzyme activity varied throughout, with greater specific activity on day one of imbibition, both in cotyledons and in the embryonic axis.Key words: Physiology, enzyme, reserves. ATIVIDADE DE -GALACTOSIDASE E MOBILIZAÇÃO DE CARBOIDRATOS EM SEMENTES DE

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Section: Carrijo L C Et Alsupporting

confidence: 53%

Section: Carrijo L C Et Almentioning

confidence: 79%

α-galactosidase activity and carbohydrate mobilization in seeds of Dalbergia nigra (vell.) Allemão ex Benth. - fabaceae (Brazilian rosewood) during germination

Carrijo

Borges

Pontes

et al. 2010

CERNE

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“…Porém é necessá-rio que a semente alcance determinado nível de umidade, cujo teor é variável com a espécie (Borges et al 2002). Diversos benefícios têm sido relatados com o emprego do osmocondicionamento, dentre eles, a maior probabilidade de se obter uma melhor germinação e emergência, particularmente em condições de estresse (Warren & Bennett 1997;Mohammadi 2009 (Souza 2009).…”

Section: Introductionunclassified

Germinação de sementes osmocondicionadas e não osmocondicionadas e crescimento inicial de Physalis angulata L. (Solanaceae) em ambientes salinos

Souza

Pelacani

2011

Acta Bot. Bras.

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Germinação de sementes osmocondicionadas e não osmocondicionadas e crescimento inicial de Physalis angulata L. (Solanaceae) em ambientes salinos RESUMO (Germinação de sementes osmocondicionadas e não osmocondicionadas e crescimento inicial de Physalis angulata L. (Solanaceae) em ambientes salinos). O gênero Physalis pertencente à família Solanaceae, possui 110 espécies, das quais 11 ocorrem no Brasil. Physalis angulata L. destaca-se no gênero pela presença de vitaesteróides, principalmente as fi salinas, encontradas em raízes, caules e folhas, com propriedades imunomoduladora, antimicrobiana e anticancerígena. Este trabalho teve por objetivo avaliar a germinabilidade de sementes osmocondicionadas e não osmocondicionadas e o crescimento inicial de P. angulata em ambientes salinos. Foram realizados experimentos nos quais as sementes foram submetidas ao osmocondicionamento (PEG 6000) e colocadas para germinar em soluções com diferentes concentrações de NaCl (2, 4, 6, 10, 12, 14 e 16 dS m -1 ). O controle (0 dS m -1 ) foi realizado com água destilada. Os parâmetros avaliados foram a germinabilidade das sementes, o índice de velocidade de germinação das sementes (IVG), o coefi ciente de uniformidade de germinação e a massa seca total das plântulas. Houve diminuição da germinabilidade e do IVG, em ambos os tratamentos. No entanto, as sementes osmocondicionadas apresentaram maior tolerância ao estresse salino. Apesar da redução signifi cativa da massa seca em relação ao aumento da salinidade do meio, o osmocondicionamento como tratamento pré-germinativo poderia ser utilizado para favorecer a tolerância ao estresse salino, uma vez que a germinabilidade foi maior para sementes osmocondicionadas.Palavras-chave: NaCl, massa seca, PEG 6000, salinidade, taxa de germinação Manuela Oliveira de Souza 1,2 , Cíntia Luiza Mascarenhas de Souza 1 e Claudinéia Regina Pelacani 1 ABSTRACT (Germination of osmoprimed and non-osmoprimed seeds and initial growth of Physalis angulata (Solanaceae) in saline environments). Th e genus Physalis belongs to the Solanaceae family, with 110 species, of which 11 occur in Brazil. Physalis angulata L. stands out in the genus due to the presence of vitasteroids, especially physalins, found in roots, stems and leaves, showing antimicrobial, anticancer and immunomodulatory properties. Th is study aimed to evaluate the germination of osmotic priming treatment and non-primed seeds and the initial growth of P. angulata in saline environments. Th e seeds were subjected to priming (PEG 6000) and incubated in solutions with increasing concentration of NaCl (2, 4, 6, 10, 12, 14 and 16 dS m -1 ). Distilled water was used as control (0 dS m -1 ). Th e parameters evaluated were seed germinability, rate of germination, coeffi cient of uniformity and total dry mass of seedlings. A decrease in germinability and IVG was observed in both treatments. However, primed seeds showed higher tolerance of salt stress. Despite the signifi cant reduction in dry mass with increasing salinity, priming as a pre-germinative treatme...

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“…The authors emphasized that glycose exerts a strong influence on the germination process, reducing the effect of ABA on the inhibition of hydrolytic enzyme synthesis, favoring activation of the α-amylase enzyme. Among the enzymes present in the embryos and cotyledons of Platymiscium pubescens, glycose was the only sugar detected during seed imbibition in PEG, and it is thought that this sugar is used in respiration and osmosis, resulting in more water absorption in the early stages of imbibition (Borges et al, 2002a). Also, in isolated embryos of Senna macranthera, a high concentration of glycose was observed in the dry seeds, with a significant increase after 72 h imbibition, with this increase being credited to the metabolism of other reserves, such as lipids and proteins (Borges et al, 2002b).…”

Section: Resultsmentioning

confidence: 99%

Activities of α-galactosidase and polygalacturonase during hydration of Dalbergia nigra ((Vell.) Fr All. ex Benth.) seeds at different temperatures

et al. 2013

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-Germination is a process that begins with seed water uptake, stimulating enzyme synthesis or activating enzymes already present. The objective of this study was to evaluate variations in monosaccharide reserves and the activities of the α-galactosidase and polygalacturonase enzymes during the hydration of two lots of Dalbergia nigra (Bahia Rosewood) seeds. Seeds from different origins constituted the two lots I and II, classified as high and low vigor, respectively. Both lots were placed in desiccators with a high relative humidity to hydrate and at 15 and 25 °C until levels of 10, 15, 20 and 25% moisture levels in seeds were reached. The seed cotyledons were analyzed for the quantity of monosaccharides and enzyme activity. The control had higher concentrations of xylose and rhamnose, which decreased during hydration until the 15% level was reached, after which concentrations increased again in both lots. Lot I, with a superior quality, showed higher glucose synthesis and degradation during seed hydration. Both enzymes were pre-existing since activity was already present in the seeds without imbibition. The polygalacturonase enzyme increased and the α-galactosidase enzyme remained relatively constant during seed hydration.Index terms: imbibition, Bahia Rosewood, biochemical modifications, monosaccharides.Atividades de α-galactosidase e poligalacturonase durante a hidratação de sementes de Dalbergia nigra ((Vell.) Fr. All. ex Benth.) sob diferentes temperaturas RESUMO -A germinação é um processo que se inicia com a absorção de água pelas sementes, estimulando a síntese de enzimas ou ativação daquelas pré-existentes. O objetivo deste trabalho foi estudar as variações nas reservas de monossacarídeos e atividades das enzimas α-galactosidase e poligalacturonase durante a hidratação de sementes de dois lotes de Dalbergia nigra (jacarandá-da-Bahia). Para tanto, sementes de duas procedências constituíram os lotes I e II, classificados como de alto e baixo vigor, respectivamente. Os lotes foram colocados para hidratar em dessecadores com alta umidade relativa nas temperaturas de 15 e 25 °C até atingirem os níveis de hidratação de 10, 15, 20 e 25% de umidade nas sementes. Os teores de monossacarídeos e as atividades das enzimas foram analisados nos cotilédones das sementes. Os teores de ramnose e xilose apresentaram valores superiores na testemunha, com redução durante a hidratação até 15%, momento a partir do qual aumentaram novamente, em ambos os lotes. O lote I, de qualidade superior, possui maior síntese e degradação de glicose durante a hidratação das sementes. Ambas as enzimas mostraram ser pré-formadas, por já apresentarem atividade nas sementes sem embebição. A poligalacturonase aumentou e a α-galactosidase manteve-se relativamente constante durante a hidratação das sementes.

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Comportamento fisiológico de sementes osmocondicionadas de Platymiscium pubescens Micheli (tamboril-da-mata)

Cited by 10 publications

References 17 publications

α-galactosidase activity and carbohydrate mobilization in seeds of Dalbergia nigra (vell.) Allemão ex Benth. - fabaceae (Brazilian rosewood) during germination

α-galactosidase activity and carbohydrate mobilization in seeds of Dalbergia nigra (vell.) Allemão ex Benth. - fabaceae (Brazilian rosewood) during germination

Germinação de sementes osmocondicionadas e não osmocondicionadas e crescimento inicial de Physalis angulata L. (Solanaceae) em ambientes salinos

Activities of α-galactosidase and polygalacturonase during hydration of Dalbergia nigra ((Vell.) Fr All. ex Benth.) seeds at different temperatures

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