Caracterização de um isolado de Bidens mosaic virus proveniente de alface

Abstract

In 2004 lettuce plants showing mosaic symptoms collected in São Manuel, SP were analyzed by electron microscopy, and particles with 730 nm typically from potyvirus were observed. After biological purification by monolesionals on Chenopodium quinoa, this isolate was sap inoculated on a host range assay. The virus infected C. quinoa and C. amaranticolor, causing local lesions and systemic mosaic. The virus did not induce symptoms on pea (Pisum sativum), but induced mosaic on the leaves of some lettuce cultivars such as Trocadero, White Boston, Regina, Verônica, Lucy Brown, Rafaela, Tainá, Vera and Laurel. The lettuce cultivar Gizele was tolerant to the virus. The coat protein gene was sequenced, and 97% aminoacids identity was observed with Bidens mosaic virus -BiMV (GenBank accession number AY960151). Differently of others previously BiMV isolates described, this isolate from lettuce was not able to infect Bidens pilosa, Helianthus annuus, Nicotiana tabacum TNN and N. glutinosa. The natural occurrence of BiMV infecting lettuce and causing mosaic symptoms similar to LMV, also the susceptibility of many lettuce cultivars to BiMV, could be an alert to the correct diagnosis of the viruses infecting this plant .

Biological and molecular characterization of bidens mosaic virus infecting patchouli (Pogostemon cablin) in Brazil

“…An isolate of BiMV identified in L. sativa plants experimentally infected Chenopodium quinoa, C. amaranticolor, P. sativum and L. sativa, while it did not infected H. annuus and B. pilosa(Suzuki et al, 2009).…”

“…To visualize the cytopathic effect on cells of patchouli plants, small pieces of symptomatic leaves were fixed, post-fixed, dehydrated, infiltrated, embedded and thin sectioned for examination with a transmission electron microscope following the procedures described by Kitajima and Nome (1999). To obtain the complete nucleotide sequence of the CP gene of the BiMV isolates, total RNA extracted from leaves of the six symptomatic patchouli plants was used in RT-PCR performed with the BiMV-specific primer pair 8331/9046, which amplifies a fragment of 715 bp from the CP gene of this potyvirus (Suzuki et al, 2009).…”

Biological and molecular characterisation of Bidens mosaic virus supports its assignment as a member of a distinct species in the genus Potyvirus

“…Bidens mosaic virus (BiMV) was described in 1961 [1] as a virus causing mosaic symptoms in Bidens pilosa L. and belonging to the genus Potyvirus. This virus is apparently restricted to Brazil and was reported to naturally infect B. pilosa, Helianthus annuus [2], Coreopsis lanceolata [3], Pisum sativum [4], Lactuca sativa [5], and Galingsoga parviflora [6]. Although BiMV was initially considered a separate potyvirus [1][2][3][4][5], it is currently classified as a strain of potato virus Y (PVY) [7] based on its coat protein (CP) gene sequence [8].…”

“…This virus is apparently restricted to Brazil and was reported to naturally infect B. pilosa, Helianthus annuus [2], Coreopsis lanceolata [3], Pisum sativum [4], Lactuca sativa [5], and Galingsoga parviflora [6]. Although BiMV was initially considered a separate potyvirus [1][2][3][4][5], it is currently classified as a strain of potato virus Y (PVY) [7] based on its coat protein (CP) gene sequence [8]. In order to clarify the taxonomic status of BiMV, we sequenced the complete genome of an isolate of the virus.…”

“…However, when their entire genomes were compared, the nt sequence identity between BiMV and BiMoV was 67%. Despite the fact that cleavage sites for HC-Pro/P3, 6K1/CI, 6K2/VPg, Vpg/NIa, and NIa/NIb are the same in SCMoV and BiMV, the biological differences in experimental host range [20,21] and the ability of BiMV to naturally infect pea and lettuce [4,5], including the emergence of a strain with distinct biological characteristics that causes severe symptoms in the latter host, support the inclusion of BiMV in a distinct species in the genus.…”