Biological and molecular characterisation of Bidens mosaic virus supports its assignment as a member of a distinct species in the genus Potyvirus

Abstract

The complete nucleotide (nt) sequence of Bidens mosaic virus (BiMV) isolate SP01 was determined and shown to consist of 9,557 nt. Since it shared highest identities in the nt sequence of the whole genome (66-73 %) and in the aa sequence of the polyprotein (60-76 %) with viruses of the potato virus Y subgroup, it was compared with them genetically and biologically. Phylogenetic analysis showed that the closest relative of BiMV is sunflower chlorotic mottle virus, from which it, however, differed significantly in various respects. These results indicate that BiMV should represent a distinct species in the genus Potyvirus.

Biological and molecular characterization of bidens mosaic virus infecting patchouli (Pogostemon cablin) in Brazil

“…The complete nucleotide sequence of the CP gene from the BiMV isolates found in patchouli plants was compared to the three available complete nucleotide sequences of CP gene of BiMV isolates in GenBank. These sequences correspond to BiMV isolates identified in infected plants of B. pilosa (GenBank AY960151, KF649336) and P. sativum (GenBank AY960150) in Brazil (Inoue‐Nagata et al., 2006; Sanches et al., 2014). The complete nucleotide sequences of the CP gene among the BiMV isolates showed identity ranging from 96.8% to 97.1% (Table 1).…”

“…The primer pairs BiMV‐CI F/BiMV‐CI R and BiMV‐HC‐Pro F/BiMV‐HC‐Pro R efficiently detected the BiMV isolates in total RNA extracted from leaves of symptomatic patchouli plants. The partial nucleotide sequences of the CI gene (GenBank OR085484, OR085485, OR085486, OR085487, OR085488 and OR085489) and HC‐Pro gene (GenBank OR085490, OR085491, OR085492, OR085493, OR085494 and OR085495) of the BiMV isolates shared, respectively, 96.90% to 97.06% and 95.78% to 96.04% identity with the sequence of a BiMV isolate (GenBanK KF649336) identified in infected plants of B. pilosa in Brazil (Sanches et al., 2014).…”

“…An isolate of BiMV identified in L. sativa plants experimentally infected Chenopodium quinoa , C. amaranticolor , P. sativum and L. sativa , while it did not infected H. annuus and B. pilosa (Suzuki et al., 2009). Conversely, the BiMV isolate identified in B. pilosa plants experimentally infected H. annuus , L. sativa , N. clevelandii , Nicotiana occidentalis , Nicotiana rustica, N. tabacum , P. sativum , Z. elegans and C. pepo (Sanches et al., 2014). Another BiMV isolate identified in C. asiatica did not infect B. pilosa ; however, it caused severe mosaic symptoms in N. benthamiana and local lesions in C. amaranticolor , and C. quinoa (Camelo‐Garcia et al., 2021).…”

“…in Brazil (Inoue-Nagata et al, 2006;Sanches et al, 2014). The complete nucleotide sequences of the CP gene among the BiMV isolates showed identity ranging from 96.8% to 97.1% (Table 1).…”

Discovery of a new polerovirus infecting Bidens pilosa

“…The weed also serves as an alternative host for several viral pathogens that cause diseases in crops. Five viruses, Bidens mosaic virus (BiMV, genus Potyvirus, family Potyviridae), Bidens mottle virus (BiMoV, Potyvirus) [1], tomato spotted wilt orthotospovirus (TSWV, genus Orthotospovirus, family Tospoviridae) [2], tomato zonate spot orthotospovirus (TZSV, Orthotospovirus) [3] pepper chlorotic spot orthotospovirus (PCSV, Orthotospovirus) [4], have been reported in B. pilosa. As one of the intermediate hosts of the above viruses, B. pilosa may play important roles in transmission and perpetuating the disease cycle of the viruses that threaten production [5].…”