Caracterização morfofisiológica de isolados do gênero Cylindrocladium

Aparecido, Christiane Ceriani; Furtado, Edson Luiz; Figueiredo, M. B.

doi:10.1590/s0100-54052008000100008

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…There was one particular consideration on nutritional status, regarding to the confirmed influence caused by transference of mycelia from a richer agar medium to a poorer one, such as performed in the present study. Aparecido et al (2008) noticed morphological changes induced by substrate changing within the same fungal isolate. It was unknown whether Hohenbuehelia species changed their mycelia pattern growth exclusively to capture nematodes so far, although our results show such changes also as consequence of mycelia transference from PDA to water-agar (2%) medium.…”

Section: Resultsmentioning

confidence: 99%

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Lubian¹,

Martinha²,

Portz³

et al. 2018

JAS

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Biological control is a method of controlling pests through the use of other living organisms. The purposes of this study were to test Hohenbuehelia species as biological control agents against Panagrellus redivivus in vitro, evaluating nematodes influence on mycelia growth; establishing daily indexes for predation and growth and setting predation percentage. Five species previously identified as 436-Hohenbuehelia mastrucata (Nematoctonus hamatus), 528-H. bullulifera (not described so far), 581-H. paraguayensis (N. sp.), 582-H. sp. (N. sp.) and 631-H. portegna (N. campylosporus) were submitted to anamorphic purification directly from basidioma. Afterwards, 100 nematodes were added to each pure colony for predation test. Evaluation started right after 24 hours of nematode-fungus interaction. Immobilized and/or penetrated nematodes were counted and mycelia growth was measured. Results were subjected to variance analyses. Hohenbuehelia mastrucata had the best performance in growth speed, followed by H. portegna and H. paraguayensis; Nematodes multiplyied much but none specie grew more as an influence of their movement under mycelium, however all species formed trap devices and some of them produced adhesive or repelent substances. Trap devices were formed in control plates also. The plates of H. paraguayensis without nematodes grew more than treatments. Cumulative predation of H. portegna was the highest at 24 (195.5%) and 48 hours (235%). At the last evaluation day, H. paraguayensis preyed the same amount (185.75%) than H. portegna, followed by H. mastrucata (109.51%). Resulst of predation daily indexes displayed chronological activity for each isolate, where H. portegna was very reactive at first 24 hours, H. mastrucata raised its predacious activity in 48 hours being constant from this time on and H. paraguayensis pointed out itself at 72 hours. Other species presented low predation and growth indexes throughout experiment.

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Section: Resultsmentioning

confidence: 99%

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Lubian¹,

Martinha²,

Portz³

et al. 2018

JAS

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“…have been detected in Brazil, most of them pathogenic to forest species and agronomic crops of major importance [15]. Ho wever, they have also been found in plants, and may be pathogenic, saprophytic, endophytic and epiphytic [16].…”

Section: Introductionmentioning

confidence: 99%

Production of α-Amylase and Amyloglucosidase by the Fungus <i>Cylindrocladium </i>sp<i>.</i> in Semi-solid State Fermentation

Onofre¹,

Groff²,

Sartori³

et al. 2012

MICROBIOLOGY

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A mylases are very important and have a wide range of applications. Semi-solid state fermentation is increasingly used for the production of enzymes, since it requires simple technology and costs are low. In this regard, it is worth emphasizing the importance of rice as a substrate, due to its readily availability and low cost. This study evaluated the ability of the fungus Cylindrocladium sp. to produce α-amylase and amy loglucosidase. The studies were carried out with the fungus strain Cylindrocladium sp. with a rice-based substrate. The material was kept in an oven at 28 °C for 96 hours and measured every 24 hours. Fo r the quantification of amy loglucosidase enzymes, the DNS reagent method was adopted. The α-amylase activity was determined by measuring the concentration of starch using iodine dosage. Analysis of the data obtained in this study showed that the fungus Cylindrocladium sp. produces α-amylase and amyloglucosidase enzymes; peak production for α-amy lase was at 72 hours with 245.02 U/g, and at 92 hours for amy loglucosidase with 73.58 U/g. Through the interpretation of these results it was possible to conclude that the fungus Cylindrocladium sp. produces relevant values of the enzy mes α-amylase and amy loglucosidase.

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Comparação de dois métodos de elaboração e validação de escala diagramática para a quantificação da severidade da mancha de Cylindrocladium em eucalipto

2014

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A mancha de Cylindrocladium em Eucalyptus (Myrtaceae) apresenta ampla ocorrência em campo, causando danos com desfolha a partir do primeiro ano de plantio. A necessidade de quantificação da doença e ausência de escalas confiáveis já publicadas, gerou a necessidade da elaboração e validação de uma escala diagramática para avaliar a mancha de Cylindrocladium spp. em cultivos de eucalipto. O estudo foi desenvolvido no município de Botucatu, São Paulo, Brasil, em talhão de E. grandis com, aproximadamente, dois anos de idade, espaçamento de 2,5 x 3,0 metros, em condições de epidemia em campo. Cem folhas de E. grandis foram coletadas e digitalizadas com uso de escâner e elaboradas duas escalas, a primeira realizada pelo software AFSoft para classificar as cores referentes às áreas saudáveis e lesionadas, e a segunda por meio de delimitação manual com auxílio de mesa digitalizadora com tratamento nos softwares GIMP e ImageJ. Após, determinou-se a severidade em função da distribuição da amostra. Para o teste de acuidade visual e validação das escalas, as folhas foram submetidas à avaliação de severidade, sem e com escala por dez avaliadores (cinco para cada escala), seguida de análise de regressão. A comparação das escalas mostrou que podem ser utilizadas para quantificar a severidade da mancha de Cylindrocladium spp. em E. grandis, com maior acurácia e precisão no segundo método. O AFSoft é indicado para elaboração de escalas com pequena variação entre as tonalidades da amostragem e menor tempo de trabalho, no contrário indica-se a delimitação manual com auxílio de mesa digitalizadora.

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Caracterização morfofisiológica de isolados do gênero Cylindrocladium

Cited by 4 publications

References 15 publications

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Production of α-Amylase and Amyloglucosidase by the Fungus <i>Cylindrocladium </i>sp<i>.</i> in Semi-solid State Fermentation

Comparação de dois métodos de elaboração e validação de escala diagramática para a quantificação da severidade da mancha de Cylindrocladium em eucalipto

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