Occurrence of Pseudomonas corrugata causing pith necrosis on tomato plants in Goiás, Brazil

Quezado-Duval, A. M.; Guimarães, Carielli M.N.; Martins, O. M.

doi:10.1590/s0100-41582007000600014

Cited by 8 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pseudomonas mediterranea was initially isolated from infected plants revealing pith necrosis in Italy, France and Spain (Catara et al, 2002), and subsequently in Por-tugal (Moura et al, 2005), Turkey (Sahin et al, 2005), Brazil (Quezado-Duval et al, 2007), Saudi Arabia (Molan et al, 2010) and Argentina (Alippi & L opez, 2010). It is a Gram-negative, non-spore-forming rod.…”

Section: Introductionmentioning

confidence: 99%

Diversity among Pseudomonas corrugata and Pseudomonas mediterranea isolated from tomato and pepper showing symptoms of pith necrosis in Greece

et al. 2014

View full text Add to dashboard Cite

Bacterial isolates from tomato plants with symptoms revealing pith necrosis collected over a 20‐year period were screened and characterized using biochemical and pathogenicity tests, as well as molecular approaches. The examined isolates were categorized into two main Pseudomonas species: P. corrugata and P. mediterranea. The diversity of these isolates was characterized through a variety of biochemical, serological, pathogenicity, DNA fingerprinting and multi‐locus sequence analysis features. Of the total Pseudomonas isolates causing pith necrosis, the biochemical profile of the selected non‐fluorescent pseudomonads consisting of P. corrugata and P. mediterranea revealed differentiation in only three characters among 43 examined. Serological analysis managed to identify the P. corrugata isolates using the laboratory prepared antiserum anti‐PC14. Genomic analyses using rep‐PCR fingerprinting and multilocus sequence analysis (MLSA) revealed considerable inter‐ and intraspecies genetic diversity. However, artificial inoculations of several plant species revealed similar pathogenicity patterns for both P. corrugata and P. mediterranea isolates. These results provide the basis for a more comprehensive understanding of the biology, sources and shift in genetic diversity and evolution of both P. corrugata and P. mediterranea populations and could support the development of molecular identification tools and epidemiological studies in diseases caused by these species. Moreover, this is the first report of P. mediterranea in Greece on tomato as well as on pepper plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Diversity among Pseudomonas corrugata and Pseudomonas mediterranea isolated from tomato and pepper showing symptoms of pith necrosis in Greece

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The symptoms caused by P. fluorescens and P. putida are identical, as well as those of the pith necrosis caused by P. corrugata (Quezado-Duval et al, 2007), thus requiring laboratory analysis to differentiate them. Such symptoms include lower leaf wilting that progresses upwards, extensive pith necrosis at the base of the plant, leaf chlorosis, dark-brown lesions, longitudinal stem cracks, adventitious root growth, and premature plant death, which are similar to the symptoms described by Aiello et al (2013).…”

Section: Resultsmentioning

confidence: 99%

“…In Brazil, the pith necrosis caused by Pseudomonas corrugata (Rodrigues Neto et al, 1989;Quezado-Duval et al, 2007), P. mediterranea (Rodrigues et al, 2010), and P. viridiflava (Monteiro Clark & Watson, 1986), P. viridiflava (Alivizatos, 1986;Malathrakis & G o u m a s , 1 9 8 7 ) , P s e u d o m o n a s fluorescens (Malathrakis & Goumas, 1987;Lo Cantore & Iacobellis, 2002;Saygili et al, 2004;Polizzi et al, 2007), Pseudomonas mediterranea (Catara et al, 2002;Trantas et al, 2015), Pseudomonas putida (Polizzi et al, 2007;Dimartino et al, 2011), and P. marginalis (Bella & Catara, 2010; P ith necrosis is a tomato (Solanum lycopersicum) disease caused by different bacteria belonging to the genus Pseudomonas. The disease is important because results in yellowing of the leaves, and impaired development of the plant that may be related to lower productivity (Monteiro et al, 2019).…”

Section: Resumo Ocorrência De Pseudomonas Fluorescens E Pseudomonas Putida Em Tomateiro No Brazilmentioning

confidence: 99%

Occurrence of Pseudomonas fluorescens and P. putida associated to tomato pith necrosis in Brazil

2021

View full text Add to dashboard Cite

In 2016, several reports emerged of fresh-market staked tomato plants with severe symptoms of pith necrosis and premature death in commercial cultivation areas in the states of Paraná and Minas Gerais, Brazil, which are similar to those caused by Pseudomonas corrugata. Four bacterial strains were isolated from infected tissue samples and characterized as Gram-negative, aerobic, and fluorescent on King’s B Medium. LOPAT tests were performed and the isolate UFU H120 was classified into group Vb (+ + − + −) and the isolates UFU H3, UFU H6, and UFU H21 into group Va (− + − + −). The pathogenicity of the isolates was confirmed by artificial inoculation on tomato plants of Santa Cruz type, cv. Kada Gigante, and Koch’s postulates were accomplished successfully. The isolates sequences of their 16S rRNA gene region were compared with those deposited in GenBank, the isolate UFU H120 aligning with Pseudomonas fluorescens (99,45% similarity) and the isolates UFU H3, UFU H6, and UFU H21 with Pseudomonas putida (99.2, 99.53 and 99.64% similarity, respectively). Notably, P. fluorescens and P. putida are most known as saprophytic bacteria normally present in the soil, although both of these species have already been reported to infect tomato plants in Italy. However, so far, there have not been any reports of such bacteria being phytopathogenic on tomato or any crop in Brazil. Thus, to our knowledge, this is the first report of pith necrosis of such occurrence in Brazil.

show abstract

“…Besides P. viridiflava [1], the bacteria Pseudomonas agglomerans [4], Pseudomonas corrugata [5], Pseudomonas mediterranea [6], Pseudomonas marginalis [7], Pseudomonas cichorii [8], Pseudomonas fluorescens and Xanthomonas perforans [9] may cause the pith necrosis of tomato. Up to now, only Pseudomonas corrugata, P. viridiflava and Pseudomonas mediterranea were reported as the etiological agent of tomato pith necrosis in Brazil.…”

Section: Introductionmentioning

confidence: 99%

“…Up to now, only Pseudomonas corrugata, P. viridiflava and Pseudomonas mediterranea were reported as the etiological agent of tomato pith necrosis in Brazil. The former was reported in the States of São Paulo [10], Rio Grande do Sul [11] and Goiás [5], P. viridiflava was reported in the State of Santa Catarina [1], and the latter was reported in the State of São Paulo [6].…”

Section: Introductionmentioning

confidence: 99%

Pith Necrosis of Tomato Caused by Pseudomonas viridiflava May Not Decrease Production

Monteiro

Ogoshi

Cardoso

et al. 2019

AJAHR

View full text Add to dashboard Cite

Aims: This study aimed to measure the losses in the production of tomato plants caused by the bacteria P. viridiflava. Study Design: Experiments were performed in a completely randomized design with six replicates. Place and Duration of Study: The study was conducted on the Caçador experimental station of the Agricultural Research and Rural Extension Enterprise of Santa Catarina (EPAGRI) from October to April during 2017/2018 and 2018/2019 crop season. Methodology: Bacteria were isolated from tomato plants with pith necrosis symptoms, using nutrient agar. The isolated strain was identified by a scheme of tests for bacteria that emit fluorescence, known as LOPAT, and by sequencing the 16S rDNA region. Tomato plants were cultivated for two seasons during 2017/2018 and 2018/2019. In the first year the cultivar Paronset was cultivated and in the next season the experiment was performed with the cultivars Compack, Nagai, Paronset and Pizzadoro. During the production season, tomato fruits were harvested and the weight was accounted for. At the beginning of the first bunch formation, the stems of the plant were inoculated with wood sticks containing bacterial colonies removed from a 48h-Petri dish culture medium. Tomato plants cultivated as control treatments were not inoculated. At the end of the cultivation seasons, the stems were cut to analyze the pith necrosis progress. Results: In both cultivation seasons, there was no decrease in the production associated with the pith necrosis caused by P. viridiflava EPAGRI BacPvT1 because the total weight of fruits harvested from inoculated plants was not statistically different compared to the non-inoculated plants. The disease progressed in all inoculated plants and adventitious root formation as external symptoms was observed. Conclusion: The bacteria Pseudomonas viridiflava EPAGRI BacPvT1, one of the etiological agents of pith necrosis of tomato, may not decrease the production. Even causing some injuries, it may be not cause any damage.

show abstract

Occurrence of Pseudomonas corrugata causing pith necrosis on tomato plants in Goiás, Brazil

Cited by 8 publications

References 0 publications

Diversity among Pseudomonas corrugata and Pseudomonas mediterranea isolated from tomato and pepper showing symptoms of pith necrosis in Greece

Diversity among Pseudomonas corrugata and Pseudomonas mediterranea isolated from tomato and pepper showing symptoms of pith necrosis in Greece

Occurrence of Pseudomonas fluorescens and P. putida associated to tomato pith necrosis in Brazil

Pith Necrosis of Tomato Caused by Pseudomonas viridiflava May Not Decrease Production

Contact Info

Product

Resources

About