Interceptação, pelo serviço de quarentena, de vírus em mudas meristemáticas de bananeiras importadas

Abstract

Quarantine Service interception of virus in imported meristem cultures of banana In 2003, banana (Musa sp.) producers located in the Northeast Region of Brazil imported a great number of micropropagated banana plantlets with the objective of enhancing production for exportation and internal consumption. Although it is an important activity for agribusiness, these importations can introduce and/or spread exotic and economically important pests to Brazil, causing serious damage to our agriculture. With the objective of minimizing the risk of virus introduction, representative samples from a group of 140,000 banana buds imported from Costa Rica were analyzed by ELISA tests at the Quarantine Laboratory of Embrapa Genetic Resources and Biotechnology (Cenargen). Banana bract mosaic virus, which is an exotic virus to Brazil and Banana streak virus were detected in the analyzed material. Although the acquisition of new genotypes is very important for the implementation of banana production in Brazil, it is shown here that the risk of introduction and dissemination of viruses associated with the material is real, and therefore, the need of phytosanitary measures to minimize those risks must be emphasized.

Clonagem e purificação de fragmento da proteína capsidial de Banana streak OL virus

“…No Brasil, o Banana streak virus (BSV) foi primeiramente descrito em bananeiras por Brioso et al (2000) e, posteriormente, por Marinho & Batista (2005), em mudas de bananeira provenientes de cultura de meristemas, importadas da Costa Rica.…”

“…Foram desenvolvidos ensaios para a detecção do BSV por PCR multiplex (Figueiredo & Brioso, 2007) e por imunocaptura seguida de reação de PCR (IC-PCR) Le Provost et al, 2006). Ndowora et al, 1999, comprovaram a presença, no genoma da bananeira, de EPRV ("endogenous pararetroviral sequences") que são sequências virais integradas ao genomas de plantas. Para o genoma da bananeira, já foram descritos dois tipos de EPRV relacionados ao BSV: um tipo que contém o genoma completo do vírus e que pode originar partículas virais e causar infecção epissomal; e outro tipo com sequências incompletas do BSV, não relacionadas à manifestação da doença (Jaufeerally-Fakim et al, 2006).…”