Establishment of a bank of positive controls for diagnosis of quarantine viruses and viroids in Brazil through PCR and RT-PCR

Duarte, Priscilla de Sousa Geraldino; Figueira, Antônia dos Reis; Galvino-Costa, Suellen Bárbara Ferreira; Sotero, Anderson de Jesus; Pompeu, Daniele Costa; Fernandes, João Ronaldo Clemente

doi:10.1016/j.cropro.2016.03.007

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…The pairs of primers for detecting the three tobamoviruses were designed to amplify the genomic fragments, located in the capsid gene by Geraldino Duarte et al (2016), as detailed in Table 1. The cDNA were synthesized from the total RNAs extracted as described before, by reverse transcription using the M-MLV RT reverse transcriptase from Promega®, following the manufacturer's instructions and the reverse primers for each virus.…”

Section: Cdna Synthesis and Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis

et al. 2018

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The transmission of tobamovirus by tomato and pepper seeds is an important mean of virus introduction in crops. Therefore, detecting its presence in the seed becomes essential for the preventive control of virus diseases. In this study, a method was proposed for the detection of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) in tomatoes (Solanum lycopersicum) and Pepper mild mottle virus (PMMoV) in pepper (Capsicum annum) seeds. Seed lots with different levels of incidence were analyzed by biological, serological, and molecular methods. Using DAS-ELISA technique, it was possible to detect TMV up to the limit

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Section: Cdna Synthesis and Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis

et al. 2018

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“…A 'stable' collection of cloned materials, either infective full-length clones or cDNAs/DNAs of relevant viral genes or parts of viral genomes, provides a cost-effective alternative to the use of live collections for reference, in particular when it comes to the monitoring of the performance of PCR tests over a given period of time (Ahlquist et al, 1984). A further advantage is that the use of cloned materials and CP extracts reduces the need for costly greenhouse facilities (De Sousa Geraldino Duarte et al, 2015). The use of plasmid-derived viral cDNA/DNA as a PAC thereby avoids the shortcomings of using freshly extracted nucleic acids from infected plants where inherent variation in virus concentration does not faithfully reflect the performance of the test.…”

Section: Common Collection For Referencementioning

confidence: 99%

“…Once the stability of the antigenicity of CP extracts has been ensured, they can be used as 'stable' positive controls in serological tests such as ELISA. A further advantage is that the use of cloned materials and CP extracts reduces the need for costly greenhouse facilities (De Sousa Geraldino Duarte et al, 2015). It also prevents the risk of cross contamination during the propagation of viruses in plants.…”

Section: Common Collection For Referencementioning

confidence: 99%

Euphresco project VirusCollect – fulfilling the need for a common collection of plant viruses and viroids for reference

Roenhorst¹,

Lacomme

Nisbet

et al. 2017

EPPO Bulletin

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The availability and accessibility of suitably characterized plant virus and viroid isolates for reference is vital for research and diagnostic laboratories. To ensure the long‐term availability of isolates and reference materials, there is a need for international collaboration. The Euphresco (European Plant Health Research Coordination) project VirusCollect aimed to establish a platform to link collections of viruses and viroids maintained by individual institutions via Q‐bank (http://www.q-bank.eu/), a database on plant pests and diseases. Within the VirusCollect project, standard operating procedures were developed and implemented by the participating laboratories to guarantee the quality of isolates and data. In addition, over 135 virus and viroid isolates of phytosanitary and/or economic importance were added to Q‐bank, which now provides links to over 500 virus isolates of almost 100 species, in addition to basic information on many more plant viruses. VirusCollect has enabled the first step in collaboration between curators and standardization of maintenance of virus collections. The project established the basis for improving the quality of individual collections and the layout of Q‐bank as a platform to share data and information. The follow‐up project, VirusCollect II, enables further international collaboration to ensure future access to reliable collections of plant viruses and viroids.

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Viroids: small entities with a mean punch

Srivastava

Prasad

2020

Applied Plant Virology

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Establishment of a bank of positive controls for diagnosis of quarantine viruses and viroids in Brazil through PCR and RT-PCR

Cited by 5 publications

References 31 publications

Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis

Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis

Euphresco project VirusCollect – fulfilling the need for a common collection of plant viruses and viroids for reference

Viroids: small entities with a mean punch

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