Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis

Almeida, João Eduardo Melo de; Figueira, Antônia dos Reis; Duarte, Priscilla de Sousa Geraldino; Lucas, Maurício Antônio; Alencar, Nara Edreira

doi:10.1590/1678-4499.2017317

Cited by 7 publications

(7 citation statements)

References 22 publications

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“…Biological, serological, and molecular assays have generally been used for identification of plant viruses. Although ELISA is preferred assay for routine virus detection, RT-PCR has increasingly been used for detection and identification of viruses due to higher level of sensitivity (Almeida et al, 2018). Among the molecular methods, the most widely used is the RT-PCR (Liu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Biological, Serological and Molecular Detection of Tomato Mosaic Virus Infecting Pepper Plants From Turkey

Çulal-Kiliç¹

2019

Appl. Ecol. Env. Res.

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This study was carried out to detect the presence Tomato mosaic virus (ToMV) by biological, serologic and molecular methods in the pepper production areas in Burdur and Denizli provinces. Symptoms related with virus infections on the pepper plants were observed during the surveys. Mechanical inoculation studies, DAS-ELISA (Double-antibody sandwich enzyme linked immunosorbent assay) tests, IC-RT-PCR (Immunocapture Reverse transcription polimerase chain reaction) studies were carried out during the work carried out to define the virus. The plants collected from the field that were suspicious with regards to being infected with the virus were first tested by the ELISA method. It was determined that 10 of the 209 tested samples were infected with ToMV. The samples that gave positive results during ELISA tests were used in the mechanical inoculation studies. Symptoms were observed in the indicator plants within a period of 7-10 days. 20 plant samples that gave negative ToMV values in the ELISA tests but had absorbance values close to positive along with the 10 samples that were tested as positive in the DAS-ELISA were used in the IC-RT-PCR method. All of the 30 samples produced expected amplification size of band with 318 bp as observed on agarose gel electrophoresis.

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Section: Discussionmentioning

confidence: 99%

“…The ELISA tests used in the diagnosis work for many plant viruses have widely been used by researchers (Geyik, 2017;Kapoor et al, 2018). The fact that it is fast, sensitive, economical and reliable makes this method a preference (Vinayarani et al, 2011;Almeida et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Biological, Serological and Molecular Detection of Tomato Mosaic Virus Infecting Pepper Plants From Turkey

Çulal-Kiliç¹

2019

Appl. Ecol. Env. Res.

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“…Sap transmission was conducted by crushing 0.1 g of diseased leaf with 1 mL buffer phosphate 0.005 M pH 7 and 10 µL mercaptoethanol. Sap was inoculated into the Chenopodium amaranticolor and Nicotiana benthamiana by mechanical inoculation (Almeida et al, 2018). The virus morphology was visualized using transmission electron microscope (JEOL JEM 1400, Japan) by a quick dipping method with negative stain using ammonium molybdat 1% pH 7 (Gebre-Selassie & Marchoux, 1991;Latifah et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

Astuti¹,

Sulandari²,

Hartono³

et al. 2021

Jurnal Hama dan Penyakit Tumbuhan Tropika

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Yellow mosaic stunt disease was found at some nurseries of Petunia in Sleman, Yogyakarta, also in Muntilan and Magelang Central Java. The disease was very important due to its ability reducing the quality and quantity of Petunia seedlings. The causal agent of the disease may be carried over to imported seeds and necessary to identify as a basic information for developing control strategies. This research was done by mechanical transmission on indicator plants. The observation of the causal agents was conducted using electron microscope with quick dipping method and the molecular detection was done using nested PCR with TobRT up1-TobRT do2 as the external primers and TobN up3-TobN do4 as the internal primers. Mechanical inoculation showed chlorosis symptoms that developed into local spot on Chenopodium amaranticolor as well as mosaic and vein banding on Nicotiana benthamiana. The observation using electron microscope showed rod-shaped virus particles sized approximately 300 nm and by PCR method produced around 568 bp and 400 bp DNA band. Based on the sequence analysis, the disease was caused by Rehmania mosaic virus. This type of Tobamovirus has 96% similarity with ReMV-Japan. ReMV, a plant pathogen which was a member of Tobamovirus that has never been reported in Indonesia. This research was the first report of ReMV in Indonesia infecting Petunia as ornamental plant.

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“…Sampai saat ini pendekatan metode serologi dan molekuler masih menjadi pilihan utama untuk melakukan deteksi virus karena kedua metode tersebut memiliki sensitivitas dan spesifitas yang tinggi (Kumar et al 2011;Kumar dan Prakash 2016;Almeida et al 2018). Walaupun demikian, kedua metode tersebut memerlukan waktu yang relatif lama, kurang efisien untuk jumlah sampel yang banyak, dan memerlukan tindakan destruktif pada tanaman.…”

Section: Pendahuluanunclassified

Metode single image-NDVI untuk deteksi dini gejala mosaik pada Capsicum annuum

Hasan¹,

Widodo²,

Mutaqin³

et al. 2021

J Fitopatol Indones

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Single image-NDVI method for early detection of mosaic symptoms in Capsicum annuum Mosaics are a symptom of a disease often found in red chilies (Capsicum annuum) and is generally caused by viral infections such as the Tobacco mosaic virus. Severe infection can cause stunting and significant yield loss. Serological and molecular detection is a common detection method for plant viruses although they are time-consuming, relatively inefficient for large samples, and are destructive to plants. On the other hand, direct symptoms observation is hampered by human visual abilities and latent symptoms in virus infection. Therefore, detection method based on the plant’s ability to absorb and reflect various spectrums of sunlight, such as the normalized difference vegetation index (NDVI), has the potential to be developed. This study aims to evaluate the potential of a single image-NDVI as an NDVI variant for the early detection of mosaic symptoms in red chilies. The main activity involved image recording of chili plants that were not inoculated (V0) and inoculated (V1) by the virus, and given minimal nutrients (M) using an unmodified RGB camera and lens filter to capture blue and Near-Infrared light reflection. Furthermore, image processing is carried out using the Photo Monitoring plugin on the Fiji-ImageJ application. The recording was done one day after inoculation (dai) until the symptoms were visible. The results showed that there was an increasing trend in the integrated NDVI value in all treatments. Howewer, the increasing trend in V1 was not significant compared to V0 and M. The difference in the mean value of integrated NDVI between V1 was very significant compared to V0 (at 5 dai) and M (at 1 dai). This method’s level of sensitivity, specificity, and accuracy ranges from 80–90% at 5 dai.

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Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis

Cited by 7 publications

References 22 publications

Biological, Serological and Molecular Detection of Tomato Mosaic Virus Infecting Pepper Plants From Turkey

Biological, Serological and Molecular Detection of Tomato Mosaic Virus Infecting Pepper Plants From Turkey

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

Metode single image-NDVI untuk deteksi dini gejala mosaik pada Capsicum annuum

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