Detection of Varicosavirus and Ophiovirus in lettuce associated with lettuce big-vein symptoms in Brazil

Colariccio, Addolorata; Chaves, Alexandre Levi Rodrigues; Eiras, Marcelo; Chagas, C. M.; Roggero, P.

doi:10.1590/s0100-41582005000400014

Cited by 10 publications

(6 citation statements)

References 7 publications

(10 reference statements)

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“…Leaves from this cultivar classified with mild and severe symptoms were positive only to the presence of MLBVV, and absorbance values obtained in these samples for LBVaV did not reach the cut-off or threshold value required to be considered positive. In several other studies, both MLBVV and LBVaV have almost always been detected in field-grown lettuce plants with big-vein symptoms (Lot et al, 2002;Roggero et al, 2003;Navarro et al, 2004;Colariccio et al, 2005;Hayes et al, 2006;Sasaya et al, 2008). In our study, ELISA results revealed that in analyzed naturally infected lettuce leaves, 100% of plants with mild, moderate, and severe symptoms were infected by MLBVV, as well as 96% of the samples were co-infected by LBVaV and MLBVV.…”

Section: Resultssupporting

confidence: 66%

“…On the other hand, lettuce plants with big-vein symptoms that are serologically negative for MLBVV, but positive for LBVaV, were described in Italy (Roggero et al, 2003). Both MLBVV and LBVaV have almost always been detected in fieldgrown lettuce plants with BVD symptoms (Roggero et al, 2003;Navarro et al, 2004;2005;Hayes et al, 2006) suggesting that LBVV may also play a role in symptom expression or disease development. In a more recent study, Sasaya et al (2008) developed methods to isolate MLBVV and LBVaV from doubly infected lettuce plants, and separately and consecutively transfer viruses to lettuce seedlings using fungal isolate zoospores.…”

Section: Bvd Is Associated With Two Viral Agents Mirafiori Lettuce Bmentioning

confidence: 99%

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Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Araya

Peña

Salazar

et al. 2011

Chilean J. Agric. Res.

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Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein and viral RNA accumulation in infected plants. Naturally infected lettuce plants, from the field or greenhouse, were classified according to their symptomatology: mild, moderate, severe, and symptomless. Coat protein accumulation was evaluated by a double antibody sandwich/enzyme-linked immunosorbent assay (DAS-ELISA), and RNA levels were studied by semiquantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Virus coat protein accumulation did not differ for the two viruses associated with this disease among lettuce plants showing different symptom severity. Similarly, abundance of Mirafiori lettuce big-vein virus (MLBVV)-RNA3 or Lettuce big-vein associated virus (LBVaV)-RNA-2 were not different (P > 0.05) for diverse big vein disease severity rating scales. This suggests that symptom severity expressed by big-vein diseased lettuce plants did not necessarily reflect the accumulation of viruses associated with this disease in the host. Therefore, lettuce plants showing mild symptoms of BVD do not necessarily present lower virus levels than plants showing more severe symptomatology.

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Section: Resultssupporting

confidence: 66%

Section: Bvd Is Associated With Two Viral Agents Mirafiori Lettuce Bmentioning

confidence: 99%

Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Araya

Peña

Salazar

et al. 2011

Chilean J. Agric. Res.

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“…Sur A. esculentus sont également présents des virus comme le Leaf curl virus (OLCV) (N'Guessan, 2001). Concernant L. sativa, il peut être infecté par plusieurs virus notamment le Lettuce big vein virus (LBVV) (Colariccio et al, 2005).…”

Section: Discussionunclassified

Contribution à l’inventaire des plantes hôtes du virus de la mosaïque du concombre dans la commune de Yamoussoukro, Côte d’Ivoire

Kouadio¹,

Agneroh²,

Kesse³

et al. 2015

Int. J. Bio. Chem. Sci

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Le virus de la mosaïque du concombre (CMV) est un virus qui constitue une très grande menace pour la réussite des cultures car il présente une répartition cosmopolite dans le monde. Une étude a été menée de mai 2011 à août 2011, dans le but de contribuer à l'identification des plantes hôtes de ce virus en Côte d'Ivoire. Trois cent vingt-huit (328) échantillons de plantes ont été collectés dans 57 parcelles, présentant des symptômes de type viral. Ces échantillons collectés ont été conservés au chlorure de calcium et testés au Double Antibody Sandwich-Enzyme Linked Immunosorbent Assay (DAS-ELISA) avec un anticorps spécifique polyclonal anti-CMV. Pour le test sérologique, 117 échantillons ont été testés. Cette étude a confirmé la présence du virus dans certaines plantes qui ont été déjà identifiées comme étant des hôtes du CMV. Ainsi, Lycopersicon esculentum, Capsicum frutescens, Vigna unguiculata, Dioscorea alata, et les adventices Chromolaena odorata, Centrosema pubescens et Solanum torvum se sont avérées positives quant à la présence du CMV. Le taux d'infection pour L. esculentum est estimé à 18,75%, C. frutescens à 33,33%, D. alata à 7,14%, V. unguiculata à 20% et les adventices à 40%. En outre, ce travail rapporte pour la première fois en Côte d'Ivoire l'infection de la plante ornementale Catharanthus roseus et des mauvaises herbes C. pubescens et S. torvum par le CMV.

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“…Quanto às viroses, destacam-se o mosaico da alface induzido pelo Lettuce mosaic virus (LMV), que é transmitido por inúmeras espécies de afídeos (Colariccio et al, 2003), o espessamento das nervuras da alface ou "big-vein", induzido pelo complexo viral Lettuce bigvein associated virus (LBVaV) e Mirafiori lettuce virus (MiLV), os dois últimos transmitidos pelo fungo de solo Olpdium brassicae (Colariccio et al, 2005). Outras viroses como o "vira-cabeça", causada por espécies de Tospovirus transmitidas por tripes, não vêm sendo relatadas como problemas fitossanitários para a cultura da alface, devido ao manejo sistemático das áreas cultivadas (Colariccio et al, 2003).…”

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Efeito da aplicação de maravilha (Mirabilis jalapa L.), primavera (Bougainvillea spectabilis L.) e isolados de Trichoderma na produção de alface

Filho¹,

Lucon²,

Duarte³

et al. 2011

Rev. bras. plantas med.

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Objetivou-se com esse trabalho avaliar o efeito de extratos foliares de primavera (Bougainvillea spetabilis L.) e maravilha (Mirabilis jalapa L.) e de Trichoderma spp., isoladamente ou em combinação, no manejo de viroses e na produção de alface. Os ensaios foram conduzidos em área de produção de hortaliças folhosas no município de Pinhalzinho/SP. O delineamento experimental foi de blocos casualizados, com dez tratamentos comparados com controle químico convencional. Foram observadas reduções de 18% e 32% na população de bactérias da rizosfera nas plantas de alface tratadas com o isolado IB18/22 e extrato de primavera + isolados de Trichoderma spp., respectivamente. Verificou-se que o extrato de maravilha estimulou o aumento do número de UFC/g da população de fungos. Entretanto, não houve diferença significativa na massa fresca da parte aérea e, apesar da pressão de inóculo e da alta densidade populacional de insetos vetores de vírus no local, não foi constatada a ocorrência de viroses, com exceção do espessamento de nervuras, que ocorreu em menor número em plantas tratadas, comparadas as do controle. Economicamente, houve 27,8% de lucratividade e margem bruta de 38,5%, indicando a viabilidade do uso de ferramentas de base ecológica no cultivo de alface. Além disso, os produtos usados são de fácil aquisição, seguros em termos de aplicação, meio ambiente e para o consumidor.

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Detection of Varicosavirus and Ophiovirus in lettuce associated with lettuce big-vein symptoms in Brazil

Cited by 10 publications

References 7 publications

Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Contribution à l’inventaire des plantes hôtes du virus de la mosaïque du concombre dans la commune de Yamoussoukro, Côte d’Ivoire

Efeito da aplicação de maravilha (Mirabilis jalapa L.), primavera (Bougainvillea spectabilis L.) e isolados de Trichoderma na produção de alface

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