103 farmers' plots located in 13 important plantain productions regions were screened. In all, 424 samples having symptoms of yellow or moderate chlorotic streaks were analyzed by reverse transcriptase-PCR and by immunocapture-PCR. Viruses identified were BSOLV, BanMMV and CMV respectively in proportions of 78%, 63% and 5.4% of the samples analyzed. Mixed infections of these three viruses were found in the 13 regions while CMV was present only in 3 regions. None of the samples collected were infected by BBrMV. Infected suckers used by farmers to establish their banana field could be the cause of these viral infections. The results showed that 9% of symptomatic samples were not associated with the presence of one or the other of the viruses studied. Further study is required to identify reported viruses in banana and plantain across the world.

In this work, we have used multispectral imaging technology to classify cassava leaves infected by African mosaic virus by the use of their unique spectral finger print. The spectra are extracted from transmission, reflection and diffusion of their multispectral images; they have been then analyzed with statistical multivariate analysis techniques. Principal component analysis (PCA) has been used followed by K-means and Ascending Hierarchical Classification (AHC) to endorse the classification. The contribution of this work is the use of multispectral imagery which binds both spatial and spectral information to differentiate and sort infected leaves. The results show that the multimodal and imaging spectroscopy may allow blind identification and characterization of infected leaves.

No virus has been reported in Kenya in cannas, and to our knowledge this is the first report of CaYMV in the country. Accepted by publication 15 January 2015. Published 25 February 2015.

This study was aimed at drawing up an inventory of the post-harvest pathogenic fungi of banana dessert and to assess their susceptibility to Azoxystrobin. A sampling was carried out in banana fruit processing factories of the main Ivorian zones of banana production (Niéky, AboissoAyamé-Akressi and Tiassalé). The identification and frequency of isolation of the fungal species associated with the affected parts of the fruit was based on their cultural aspects, reproductive structures and percentage of contamination. The susceptibility of post-harvest fungi to different doses of Azoxystrobin 3.5, 4 and 4.5 g/hl was compared to a reference product, Bitertanol at a dose of 2 g/hl and to a check where only water is applied. Of the 13 pathogenic fungal species isolated, Botryodiplodia theobromae was the most recurrent and was identified as the main causal agent of postharvest rots. It is followed by Colletotrichum musae, Aspergillus niger, Fusarium moniliforme, Geotrichum candidum, Fusarium semitectum and Aspergillus flavus, in which the isolation frequencies are significantly different. The results on the susceptibility of pathogenic fungi to Azoxystrobin show that the three doses of Azoxystrobin have significantly different effects from that of the reference product and the check. They reduce infection rates up to 91.67% and give a high proportion of marketable fruits, respectively. Surprisingly, the dose of the reference product gives a low rate of reduction of infections (49%) and a proportion of marketable fruit that is significantly identical to that of water treatment.

oculated Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana, N. tabacum (cvs. Havana, Glurk and Turkish), N. glutinosa. Datura stramonium, common bean (cvs. Topcrop and Pinto bean), pumpkin (cv. Small Sugar), pepper (Capsicum annuum, cv. Yolo Wonder) and cucumber (cvs. Emparator and Poinsett 76) plants (results of three independent experiments with six plants per experiment). Symptomless infections were detected in pepper (24 of 30), N. benthamiana (25 of 25), and N. tabacum cv. Turkish (11 of 24) plants by RT-PCR with the pCLV4/pCLVR4 primer pair. To our knowledge, this is the first report of CLVd infecting tomato in Mali. RT-PCR tests of seeds collected from CLVd-infected tomato, pepper, and N. benthamiana plants also detected CLVd (1). Thus, it is possible that CLVd was introduced into Mali in association with seed.

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Objective: Eggplant (Solanum melongena) is one of the important vegetables in Africa and Asia. Begomoviruses are emerging plant viruses that cause significant losses. However, there is little research on begomoviruses infecting eggplant. Therefore, this study aimed at identifying begomoviruses infecting eggplant. Methodology and results: Six samples of virus-like infected eggplants were collected in Ferkessedougou in the North of Cote d’Ivoire. The molecular tests Polymerase Chain Reaction (PCR) and Rolling Circle Amplification (RCA) were performed on the samples. One sample tested positive by PCR and RCA while the five others were negative by PCR for begomoviruses. Products from both tests were sequenced to get partial sequence of begomovirus Pepper yellow vein Mali virus (PepYVMLV) from PCR and two full genome components DNA A and DNA B of PepYVMLV from RCA. The sequences were released in Genbank. Conclusion and application of findings: This study has done the molecular characterization of the complete two genome sequence components DNA A and DNA B of Pepper yellow vein Mali on eggplant. Agro-infection of eggplants with the two components could reveal actual specific symptoms which are caused by PepYVMLV on eggplant. This could help opens possibilities of engineering resistant eggplant to PepYMLV. Keywords: Eggplant, begomovirus, Pepper yellow vein Mali virus, new host, Cote d’Ivoire RESUME Objectif: L’aubergine (Solanum melongena) est l’un des légumes les plus importants en Afrique et en Asie. Les begomovirus sont des virus émergents qui causent de pertes importantes. Toutefois, il y a très peu de recherches sur les begomovirus de l’aubergine. Ainsi, cette étude visait à l’identification des begomovirus infectant l’aubergine. Méthodologie et résultats: Nous avons collecté 6 échantillons d’aubergine à Ferkéssédougou au Nord de la Côte d’Ivoire, parmi des plants d’aubergine qui présentaient des symptômes de type viral. Les tests moléculaires Polymerase Chain Reaction (PCR) et Rolling Circle Amplification (RCA) ont été réalisés sur les échantillons. Un échantillon a été positif à la fois à la PCR et la RCA alors que les 5 autres étaient négatifs à la PCR pour les begomovirus. Le séquençage des produits de la PCR a donné une séquence partielle du Soro et al., J. Appl. Biosci. 2021 Identification of eggplant (Solanum melongena) as a new host of begomovirus Pepper yellow vein Mali virus in Côte d’Ivoire 16154 begomovirus Pepper yellow vein Mali virus (PepYVMLV). Les produits issus de la RCA ont donné des séquences des composants ADN A et ADN B de PepYVMLV qui ont été publiées dans le Genbank. Conclusion et application des résultats: Notre étude a effectué la caractérisation moléculaire des deux sequences complètes des composantes DNA A et DNA B du génome complet du Pepper yellow vein Mali virus sur l’aubergine. L’agro-infectioon des aubergines avec les deux composantes pourrait révéler les symptômes spécifiques réels qui sont causés par le PepYVMLV sur l’aubergine. Cela pourrait ouvrir des possibilités de mise en place de variétés d’aubergines résistantes au PepYMLV. Mots-clés: Aubergine, begomovirus, Pepper yellow vein Mali virus, nouvelle plante hôte, Côte d’Ivoi