Técnica de restrição hídrica: efeito sobre Acremonium strictum, protrusão de sementes e obtenção de sementes de milho infetadas

Teixeira, Hudson; Machado, José da Cruz; Oride, Denis; Alves, Marcelo de Carvalho; Noda, Alexandre

doi:10.1590/s0100-41582005000200001

Cited by 5 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The increase in the pathogen incidence on inoculated seeds through all inoculum potentials proved the efficiency of this methodology which allowed the presence of the pathogen in high levels in seeds. Accordingly, similar results and observations from other papers support this as the most appropriate technique (Teixeira and Machado, 2003;Teixeira et al, 2005;Araújo et al, 2006;Sousa et al, 2008).…”

Section: Resultssupporting

confidence: 87%

Transmission and effects of Fusarium oxysporum f. sp. vasinfectum on cotton seeds

Araújo¹,

Machado²,

Pedrozo³

et al. 2016

Afr. J. Agric. Res.

View full text Add to dashboard Cite

This paper aimed to evaluate the transmission of Fusarium oxysporum f. sp. vasinfectum (FOV) and the effects of this pathogen on the initial development of cotton plants following inoculation of seeds. Two cultivars (susceptible and resistant) and two strains (most and least aggressive) of the pathogen were used in this study. The inoculation method was based on the contact between seeds and fungal colonies on substrates containing mannitol. Percentage of FOV in seeds and the percentage of seed germination were evaluated by blother test and germination test, after inoculation. Emergence of seedlings and speed index, initial and final stands, size and dry weight of the plants were verified in trays containing soil substrate. Disease severity, pathogen transmission and plant infection, from seed to plant, were determined in separate trial on plants. Occurrence of the pathogen was higher when inoculum potential was increased for all variables analyzed. The number of normal seedlings, determined by seed germination test, decreased when the incidence of the pathogen in the seed was increased. The same occurred to other variables, in which there was difference between cultivars where IAC 20-233 presented the best performance. No significant differences were found between strains for emergence speed index, initial and final stands variables. Transmission and infection rates were increased according to the inoculum potentials increasing and the maximum pathogen transmission rate, from seed to plant was around 50%.

show abstract

Section: Resultssupporting

confidence: 87%

Transmission and effects of Fusarium oxysporum f. sp. vasinfectum on cotton seeds

Araújo¹,

Machado²,

Pedrozo³

et al. 2016

Afr. J. Agric. Res.

View full text Add to dashboard Cite

show abstract

“…In this study, there was no effect of A. alternata isolates. According to earlier reports, fungal development may be stimulated at potentials varying from -0.3 to -2.0 MPa (Adebayo & Harris, 1971;Wearing & Burgess, 1979;Subbarao et al, 1993;Gao & Shain, 1995;Whiting & Rizzo, 1999;Carvalho et al, 2001;Farias et al, 2004;Teixeira et al, 2005;Machado et al, 2008). On the other hand, at more negative potentials than -1.0 MPa the growth of some fungi can be affected by some of the solutes used (Alam et al, 1996).…”

Section: Mycelial Growth On Osmotically Modified Agar Mediummentioning

confidence: 96%

“…Growth of Colletotrichum lindemunthianum, Bipolaris sorokiniana, Acremonium strictum, Drechslera oryzae, Phoma sorghina, Pyricularia grisea, Gerlachia oryzae, Fusarium oxysporum f.sp. phaseoli, Macrophomina phaseolina and Rhizoctonia solani increased progressively as the osmotic potential increased in the range varying from -0.3 MPa to -1.0 MPa (Carvalho et al, 2001;Farias et al, 2004;Teixeira et al, 2005;Machado et al, 2008). The effect was independent of the solute type, either salt or sucrose, in the agar medium.…”

Section: Introductionmentioning

confidence: 99%

Use of osmotic solutions for inhibition of sunflower seed germination in blotter test

Falleiro

Almeida

Coutinho

et al. 2010

Trop. plant pathol.

View full text Add to dashboard Cite

The aim of this work was to investigate the feasibility of using a water restriction technique with osmotic solutes to control sunflower (Helianthus annuus L.) seed germination in blotter tests. The effects of water potential (-0.8 MPa -Megapascal, -1.0 MPa, -1.2 MPa and -1.4 MPa) of substrates osmotically modified with sodium chloride or mannitol were evaluated for seed germination, radicle length and mycoflora present in the samples. The effects of levels of osmotic potentials ranging from -0.35 MPa to -1.4 MPa were also investigated on mycelial growth of the fungi Alternaria alternata and Alternaria zinniae on PDA medium. All osmotic potentials were able to inhibit or to retard seed germination to the extent that seed examination in stereomicroscope became easier without affecting seed mycoflora associated with the seeds. Mycelial growth of A. alternata was stimulated by all osmotic solutes tested, while mycelial growth of A. zinniae was stimulated by mannitol and reduced by all NaCl osmotic potential levels used. Key words: Helianthus annuus, sodium chloride, mannitol. RESUMO Uso de solutos osmóticos na inhibição da germinação de sementes de girasol em testes de sanidade ("blotter test")Neste trabalho, o objetivo foi avaliar o uso de solutos osmóticos em testes de sanidade de sementes de girassol pelo método de incubação em papel de filtro com restrição hídrica do substrato, induzida pela adição de cloreto de sódio e manitol, em relação à germinação de sementes, comprimento de radícula e a detecção da micoflora associada às sementes ou plântulas. Foram testados os níveis de restrição hídrica de -0,8 MPa (Megapascal), -1.0 MPa, -1,2 MPa e -1,4 MPa. O crescimento micelial in vitro dos fungos Alternaria alternata e Alternaria zinniae foi também avaliado em meio de BDA osmoticamente modificado com os mesmos solutos e níveis de restrição, variando de -0,35 MPa (BDA padrão) a -1,4 MPa. A restrição hídrica do substrato de papel induzida pelos solutos testados, nos níveis avaliados, reduziu a germinação das sementes e o comprimento de radícula e não interferiu na detecção dos fungos. A restrição hídrica do meio BDA, induzida por manitol nos diferentes potenciais osmóticos testados, estimulou o crescimento micelial tanto de A. alternata quanto de A. zinniae, enquanto que o meio modificado com NaCl estimulou apenas o crescimento micelial de A. alternata, afetando adversamente o crescimento micelial de A. zinniae. Palavras-chave: Helianthus annuus, cloreto de sódio, manitol.

show abstract

“…According to the authors, the expressive rise in the number of abnormal seedlings and dead seeds is a reflex of the higher inoculation potential, as a consequence of the more extended contact with the pathogen. Nevertheless, seeds exposed to high osmotic potentials for too long may lose their germination capacity, as Teixeira et al (2005) Means followed by the same letter statistically do not differ, according to Tukey's test at a 5% probability level.…”

Section: Germination Testmentioning

confidence: 99%

Use of water restriction in the inoculation of carrot seeds

Pereira

Torres²,

Silva

et al. 2019

J. Seed Sci.

View full text Add to dashboard Cite

This study aimed at evaluating the method of inoculation of carrot seeds in a potato-dextrose-agar medium (PDA) added of mannitol. Alternaria radicina was the fungus chosen to test such methodology. The osmosis-controlling agent was employed to obtain the osmotic potentials of 0.6 MPa, 0.8 MPa, 1.0 MPa and 1.2 MPa. In comparison with the control treatment, no significant mycelial growth inhibition was noticed at any of the osmotic potentials considered. The seeds were distributed over the fungal colonies for infection, where they remained for 48, 72, 96, 120 and 144 hours, at the osmotic potentials of 0.0 MPa (control), 0.6 MPa, 0.8 MPa, 1.0 MPa and 1.2 MPa, respectively. Tests of germination, sanitization and emergence were performed to evaluate the effects of colonization. Regarding the germination, the different osmotic potentials resulted in similar percentages of abnormal seedlings and dead seeds. As for the sanitization testing, higher infection rates were observed at the osmotic potentials of 1.0 MPa and 1.2 MPa. The lowest percentage of emergence was found at -1.2 MPa, therefore suggesting that this osmotic potential (produced with mannitol) might be used for infecting carrot seeds for research purposes.

show abstract

Técnica de restrição hídrica: efeito sobre Acremonium strictum, protrusão de sementes e obtenção de sementes de milho infetadas

Cited by 5 publications

References 9 publications

Transmission and effects of Fusarium oxysporum f. sp. vasinfectum on cotton seeds

Transmission and effects of Fusarium oxysporum f. sp. vasinfectum on cotton seeds

Use of osmotic solutions for inhibition of sunflower seed germination in blotter test

Use of water restriction in the inoculation of carrot seeds

Contact Info

Product

Resources

About