Distinct features of Pepper yellow mosaic virus isolates from tomato and sweetpepper

Abstract

Determination of virus diversity in the field is vital to support a sustainable breeding program for virus resistance of horticultural crops. The present study aimed to characterize four field potyvirus isolates found naturally infecting sweet pepper (Capsicum annuum) (Sa66 and Sa115) and tomato (Lycopersicon esculentum) (IAC3 and Sa21) plants. Their biological characteristics revealed differences among the isolates in their ability to infect distinct Capsicum spp. and tomato genotypes, and in the severity of symptoms caused by these isolates compared to the infection caused by an isolate of Pepper yellow mosaic virus (PepYMV). Absence of cross-reaction was found among the studied isolates with antiserum against Potato virus Y (PVY). However, all isolates reacted, at different intensities, with antiserum against PepYMV. All isolates showed high identity percentage (97 to 99%) of the amino acid sequence of the coat protein with PepYMV (accession AF348610) and low (69 to 80%) with other potyvirus species. The comparison of the 3' untranslated region also confirmed this finding with 97 to 98% identity with PepYMV, and of 47 to 71% with other potyviruses. The results showed that PepYMV isolates were easily differentiated from PVY by serology and that the host response of each isolate could be variable. In addition, the nucleotide sequence of the coat protein and 3' untranslated region was highly conserved among the isolates.

Integrated control of pests in tropical and subtropical sweet pepper production

“…The green peach aphid, Myzus persicae (Sulzer), causes direct damage to leaves by the secretion of phytotoxins 25. General aphid damage is indirect: sooty moulds grow on secreted honeydew, and many aphids can transmit potyviruses 3. Another aphid pest found in subtropical covered peppers is Aphis gossypii (Glover) 26.…”

“…Sweet pepper ( Capsicum annuum L.) is an important crop in all parts of the world. In open fields, where yield is ⩽12 t ha −1 , sweet pepper is subject to a number of pests, including beetles,1 caterpillars,2 aphids3 and thrips 4. As an alternative to exclusively treating pests with insecticides, integrated pest management (IPM) includes various cultural, biological and chemical tactics.…”

Genome-Wide Analysis of Differentially Expressed Genes During the Early Stages of Tomato Infection by a Potyvirus

“…Pepper yellow mosaic virus (PepYMV) is a typical potyvirus recently described infecting sweet pepper (Capsicum annnum) (Inoue-Nagata et al 2002) and tomatoes (Solanum lycopersicum) (Cunha et al 2004). It is closely related to Potato virus Y (PVY), to such an extent that PVY and PepYMV isolates cannot be differentiated based on host range and symptomatology (Truta et al 2004).…”

Plant Health Newsletter on Horizon Scanning – September 2022

“…Pepper yellow mosaic virus is a pest of solanaceous plants, such as peppers (Moura et al, 2011) 9 , tomatoes (da Cunha et al 2004) 10 and garden petunias (Duarte et al, 2021) 11 . The aphid Aphis gossypii has been associated with the transmission of this virus (Souza et al 2020) 12 .…”