Variabilidade genética de isolados do complexo Colletotrichum associados a sementes de algodoeiro, por meio de técnicas moleculares e inoculação em plantas

Silva-Mann, Renata; Salgado, Kalinka C. C.; Vieira, M.G.G.C.; Machado, José C.

doi:10.1590/s0100-41582002000100004

Cited by 13 publications

(9 citation statements)

References 10 publications

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“…cephalosporioides mantidos na micoteca do Laboratório de Patologia de Sementes do Departamento de Fitopatologia na Universidade Federal de Lavras (UFLA) (Tabela 1). Os isolados foram repicados em placas com BDA e mantidos em condições controladas de temperatura, umidade e iluminação (Silva-Mann et al, 2002).…”

Section: Isolados Fúngicosunclassified

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Compatibilidade sexual e vegetativa do complexo Glomerella-Colletotrichum associado a sementes de algodão

et al. 2004

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RESUMOColletotrichum gossypii (Cg) (Glomerella gossipii) e Colletotrichum gossypii var. cephalosporioides (Cgc) são agentes etiológicos da antracnose e da ramulose no algodoeiro (Gossypium hirsutum). Ambos os patógenos são encontrados na forma mitospórica na natureza e a diferenciação entre eles é feita principalmente a partir dos sintomas das doenças causadas. O presente trabalho foi realizado com o objetivo de verificar a ocorrência de reações de compatibilidade sexual e incompatibilidade vegetativa entre e dentro de isolados de Cg e Cgc e avaliar alguns aspectos citológicos das formas de esporulação.Pelos resultados, observou-se existência de grande variabilidade dentro dessa espécie. Os conídios diferiram em tamanho em todos os isolados, sendo encontrados conídios bi-e trinucleados. Em cultura, nas áreas de contato (entre as hifas), foram observadas reações de incompatibilidade vegetativa tanto entre quanto dentro dos isolados. Em apenas um dos isolados avaliados ocorreu reprodução sexuada de forma espontânea, sugerindo comportamento homotálico.Palavras-chave adicionais: anastomose, antracnose, ramulose, citogenética, reprodução sexual. ABSTRACT Sexual and vegetative compatibility of Glomerella-Colletotrichum complex associated with cotton seedsColletotrichum gossypii (Cg) (Glomerella gossypii) and Colletotrichum gossypii var. cephalosporioides (Cgc) are pathogens of anthracnose and ramulose in cotton (Gossypium hirsutum). These pathogens cause typical and distinct symptoms in susceptible plants and they occur in the mitosporic phase of hosts in nature. This work aimed to check the occurrence of sexual and vegetative incompatibility reactions between and within isolates of Cg and Cgc, and also to study some cytological aspects involving the sporulation process. A large variability was observed within these pathogens. Conidia were differentiated in size in all isolates and some presented two or three nuclei. Vegetative incompatibility between and within isolates took place at the contact lines of mycelia growth. Spontaneous sexual reproduction was found in only one isolate, indicating the homothallic behavior of this isolate.

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Section: Isolados Fúngicosunclassified

“…cephalosporioides (Costa & Fraga) (Cgc) quando causa a ramulose no algodoeiro. Ambos os fungos são semelhantes morfologicamente, e a diferenciação entre eles baseia-se principalmente nos sintomas da doença causada nas plantas de algodoeiro e/ou com o uso de marcadores moleculares (Tanaka et al, 1996;Cia & Salgado, 1997;Silva-Mann et al, 2002).…”

Section: Introductionunclassified

Compatibilidade sexual e vegetativa do complexo Glomerella-Colletotrichum associado a sementes de algodão

et al. 2004

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“…Most of the studies on genetic variability of strains from the Colletotrichum complex in cotton are based on polymorphism among randomly amplified DNA fragments (CHITARRA, 1996;VIEIRA, 1996;MEHTA et al, 2001;SILVA-MANN et al, 2002, 2005 and isozyme patterns (VIEIRA, 1996;CARVALHO et al, 1997). From the results of those studies, it is concluded that the primers used might be unable to distinguish members of those groups of fungi associated with cotton seeds.…”

Section: Introductionmentioning

confidence: 99%

Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis

Carvalho¹,

Figueira²,

Machado³

et al. 2015

Biosci. J.

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ABSTRACT:The use of DNA sequences analysis has been an important mean to distinguish and to identify populations of organisms at different levels. By molecular markers several complex organisms have been successful detected in plants for distinct aims. Ribosomal DNA (rDNA) has been used to evaluate genetic variability, microorganism phylogeny and to develop specific primers for detection of plant pathogens in plant tissues. In this study, the objective was to characterize isolates of Colletotrichum gossypii var. cephalosporioides and Colletotrichum gossypii, collected in different regions of Brazil, by analyzing the nucleotide sequence of rDNA regions. ITS1, ITS2, and the intervening 5.8S gene were amplified by PCR and their sequences compared to each other and to those from other species registered in the GenBank. The rDNA of isolates associated with Gossypium spp. showed sequence identities ranging from 96 to 100% in the ITS1 region, 98 to 100% in the 5.8S gene, and 97 to 100% in the ITS2 region. The sequences were submitted to UPGMA analysis, and according to the phylogenetic trees, the C. gossypii var. cephalosporioides and C. gossypii species clustered together along with isolates of Glomerella cingulata from mango and papaya, and thus no distinction could be made between isolates of those organisms.

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“…These markers can be affected by many environmental factors and especially by stress. In contrast, heat resistant proteins are potential markers that do not present any known catalytic activity (Silva-Mann et al, 2002) presenting polymorphism and stability in seeds, such as in maize (Roveri José et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Physiological quality and protein patterns of corn seeds produced under water and salt stresses

Diniz¹,

Pinho²,

Santos³

et al. 2015

Afr. J. Agric. Res.

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This study evaluated the effect of water and salt stresses on the physiological quality and on the electrophoresis patterns of heat resistant proteins in corn seeds. The experiments were carried out in the laboratories of seed analysis and biotechnology at the Universidade Federal de Lavras. Corn seeds of the hybrid GNZ 2004 and the inbred line LE 57 were used. The seeds were produced in soils with an electric conductivity of 3 dS m -1 (under stress) and 0.4 dS m -1 (without stress) and in pots containing substrate with a water holding capacity of 40% (with stress) and 70% (without stress). The randomized complete block design with treatments arranged in a split-plot scheme was used with four replications. Seeds were harvested at different stages of development. Seed physiological quality was evaluated using the tests of germination, artificial aging and cold test. The patterns of heat resistant proteins were evaluated by electrophoresis. The results showed that depending on seed developmental stage there was an effect of water and salt stresses on the seed vigor. The electrophoretical patterns of heat resistant proteins were stable in seeds produced under different stress conditions.

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Variabilidade genética de isolados do complexo Colletotrichum associados a sementes de algodoeiro, por meio de técnicas moleculares e inoculação em plantas

Cited by 13 publications

References 10 publications

Compatibilidade sexual e vegetativa do complexo Glomerella-Colletotrichum associado a sementes de algodão

Compatibilidade sexual e vegetativa do complexo Glomerella-Colletotrichum associado a sementes de algodão

Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis

Physiological quality and protein patterns of corn seeds produced under water and salt stresses

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