Toxicidade de filtrados fúngicos a meloidogyne incognita

Costa, Mauro Junior Natalino da; Campos, Vicente Paulo; Pfenning, Ludwig H.; Oliveira, Denilson F.

doi:10.1590/s0100-41582001000400011

Cited by 18 publications

(18 citation statements)

References 7 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Czapeck-dox liquid medium, which is chemically defined, was suitable for stimulating the production of this type of metabolite produced by Fusarium, in accordance with reports by other investigators (18)(19)(20)(21)(22)(23)(24)(25).…”

Section: Discussionsupporting

confidence: 85%

“…This same type of extract, obtained from cultures of F. oxysporum in the plant studies of Melo and Piccinin (18), provoked reactions that produced withering in cucumber cells and plantlets, and cell death. Another study evaluating the effects of filtrates from different fungal species on the eclosion, motility and mortality of phytonematodes demonstrated that the F. oxysporum filtrate showed greater toxic activity than the filtrates obtained from other species (19).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats

Hernandes¹,

Marangon²,

Salci³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Abstract:The genus Fusarium is known to produce mycotoxins that cause fusariosis in plants, animals and humans. Mycotoxins are among the virulence factors of this genus. Metabolic extracts of Fusarium oxysporum, isolated from a patient with onychomycosis and sterilized by filtration or autoclave, were inoculated intradermally into Wistar rats at concentrations of 0.25, 0.5 and 1 μg/μL, and the effects on their tegument were observed at 24 and 72 hours. After histological procedures and staining by hematoxylineosin, the sections were studied for their inflammatory-reaction intensity and for evidence of injury and tissue distortion. Inflammatory reactions in the dermis and the subcutaneous tissue were observed at all concentrations of the inoculated extract tested. There was a significant influx of neutrophils, mastocytes and lymphocytes, as well as a large quantity of macrophages. Apoptotic bodies and hyperemic blood vessels were observed. This reaction was directly related to the extract concentration, and was most intense in animals that received the 1 mg/µL dose. The maximum peak was observed at 24 hours. The autoclaved metabolic extract produced the same effects as the untreated one, indicating the presence of heat-resistant metabolites. In conclusion, the metabolic extracts obtained from sterilized culture filtrates of F. oxysporum are capable of inducing an inflammatory response within 24 hours in the dermis and subcutaneous tissue of rats.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats

Hernandes¹,

Marangon²,

Salci³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…A suspensão foi autoclavada (121 o C, 30 min) e depois filtrada de acordo com Costa et al (2001). Os organismos Bacillus subtilis e Pseudomonas putida foram isolados de folhas de café conforme recomendações de Maringoni (1995).…”

unclassified

Avaliação de produtos alternativos no controle da ferrugem do cafeeiro

2007

Self Cite

View full text Add to dashboard Cite

de produtos alternativos no controle da ferrugem do cafeeiro. Fitopatologia Brasileira 32: 150-155. 2007. RESUMOObjetivou-se neste trabalho avaliar produtos orgânicos quanto ao efeito protetor e indutor de resistência à ferrugem do cafeeiro (Hemileia vastatrix), comparando-se com fungicidas sistêmicos. O trabalho foi desenvolvido em casa-de-vegetação, utilizando-se mudas de cafeeiro cv. Catuaí Vermelho 144. Foram avalidados como indutores de resistência extratos foliares, suspensões de conídios fúngicos e de células bacterianas, fertilizantes foliares, hipocloritos e acibenzolar-S-methyl (ASM). O delineamento experimental utilizado foi em blocos casualizados com três repetições, sendo que cada parcela experimental foi constituída de cinco mudas de café perfazendo um total de 15 mudas por tratamento. Os experimentos foram repetidos duas vezes com resultados semelhantes. Quando avaliado o efeito protetor, a maioria dos compostos reduziu o número de pústulas e a área foliar com ferrugem em comparação à testemunha 1 (água). Extratos de folhas de café, obtidos pelo processo aquoso e extratos de sementes de Azadirachta indica ('neem") obtidos pelo processo de extração com metanol apresentaram proteção similar, mas nunca superior a epoxiconazole + piraclostrobin. Extratos aquosos de folhas de café, ASM, Bacillus subtilis e Pseudomonas putida reduziram a infecção causada por H. vastatrix em mais de 77 %.Palavras-chave adicionais: controle alternativo de doenças, efeito protetivo, Coffea arábica, Hemileia vastatrix. ABSTRACT Evaluation of alternative products and fungicides for the control of coffee leaf rustThe main objective of this study was to evaluate the use of organic products for their protective effect against coffee leaf rust (Hemileia vastatrix Berk. & Br.) and also their ability to induce resistance , compared to systemic fungicides. This study was carried out under greenhouse conditions using the cv. 'Catuai Vermelho 144'. Leaf extracts, suspensions of fungal conidia and bacterial cells, foliar fertilizers, hypochlorites and acibenzolar-S-methyl (ASM) were evaluated as potential inducers of resistance. The experiment was installed in a complete randomized block design with three replications. Each experimental plot contained five coffee plants and each treatment had 15 plants. The experiments were repeated twice with similar results. With regard to the protective effect, most of the treatments decreased the number of pustules and the leaf area with rust in comparison to treatment 1 (leaves sprayed with water). Water extracts of coffee leaves and methanol extracts of seeds of Azadirachta indica ("neem") gave similar results, but never better than the protection obtained by using epoxiconazole + piraclostrobin. Regarding induction of resistance, extracts of coffee leaves, ASM, Bacillus subtilis and Pseudomonas putida reduced the infection caused by H. vastatrix by more than 77 %.

show abstract

“…Esses autores concluíram que o somatório dos efeitos de substâncias diferentes exige tempo para sua expressão e, portanto, o período de avaliação de 48 horas seria mais apropriado. Costa et al (2001) verificaram, com o filtrado do fungo Paecilomyces lilacinus, que, ao passar da diluição de 1:2 para 1:3 (filtrado:água), houve queda na porcentagem de J2 de M. incognita mortos, passando de aproximadamente 95% para 30%. Park et al (2002) observaram porcentagens de redução da mobilidade de Caenorhabiditis elegans entre 50 e 90%, em períodos de 3 e 6 horas de incubação, respectivamente, quando colocados diretamente na colônia de Streptoverticillium albireticuli, cultivado em placas de Petri por sete dias no meio FMEA.…”

Section: Resultsunclassified

Estreptomicetos no controle da meloidoginose em mudas de tomateiro

Sousa

Soares

Garrido

et al. 2006

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -Este trabalho teve como objetivo avaliar o efeito de seis isolados de estreptomicetos na mortalidade e eclosão de juvenis de segundo estádio (J2) de Meloidogyne incognita e no controle da meloidoginose em mudas de tomateiro. Foi montado um bioensaio em placas tipo Elisa, sendo adicionados em cada célula, 200 µL de metabólitos dos isolados, com 20 µL de uma suspensão com 25 juvenis de segundo estádio (J2) de M. incognita. Os metabólitos produzidos por Streptomyces griseus subsp. griseus causaram 98,2% de mortalidade dos J2 de M. incognita. Em outro bioensaio, foram adicionados 3 mL dos metabólitos em frascos de vidro, com 100 µL da suspensão contendo 25 ovos de M. incognita. O isolado N0035 de Streptomyces proporcionou 98,8% de inibição na eclosão de J2 de M. incognita. Num terceiro bioensaio, o substrato de produção de mudas foi infestado com suspensão de estreptomicetos e incubado por 30 dias. Quinze dias depois da germinação das sementes do tomateiro, foi realizada a inoculação com 2.000 J2 por planta. Verificou-se a redução de 68% no número de galhas por grama de raiz e de 76,8% na massa de ovos por grama de raiz, nas mudas produzidas no substrato infestado e incubado com Streptomyces griseus subsp. griseus, quando comparado com a testemunha.Termos para indexação: Meloidogyne incognita, Lycopersicon esculentum, Streptomyces, actinomicetos, nematóide-das-galhas, biocontrole. Streptomycetes in the control of Meloidogyne incognita in tomato plantsAbstract -This work aimed to evaluate the effect of six streptomycete isolates on mortality, hatching, and control of Meloidogyne incognita on tomato seedlings. An assay was conducted in Elisa plates, with the addition of 200 µL of the streptomycete metabolites, along with 20 µL of suspension containing 25 second stage juveniles (J2) of M. incognita. The metabolites produced by Streptomyces griseus subsp. griseus caused mortality of 98.2% on M. incognita. A second assay was conducted with 3 mL of streptomycete metabolites and 25 eggs of M. incognita. The isolate N0035 of Streptomyces caused inhibition of 98.8% on hatching. In a third assay, potting mix was infested with suspension of the streptomycetes isolates and incubated for 30 days. Fifteen days after germination, tomato seedlings were inoculated with a suspension containing 2,000 J2 of M. incognita. Reductions of 68% in the number of galls per gram of roots and of 76.8% in the egg mass per gram of roots were observed on tomato seedlings grown in the potting mix inoculated with Streptomyces griseus subsp. griseus, compared to the control.

show abstract

Toxicidade de filtrados fúngicos a meloidogyne incognita

Cited by 18 publications

References 7 publications

Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats

Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats

Avaliação de produtos alternativos no controle da ferrugem do cafeeiro

Estreptomicetos no controle da meloidoginose em mudas de tomateiro

Contact Info

Product

Resources

About