2012
DOI: 10.1590/s1678-91992012000200003
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Toxic thermoresistant metabolites of Fusarium oxysporum are capable of inducing histopathological alterations in Wistar rats

Abstract: Abstract:The genus Fusarium is known to produce mycotoxins that cause fusariosis in plants, animals and humans. Mycotoxins are among the virulence factors of this genus. Metabolic extracts of Fusarium oxysporum, isolated from a patient with onychomycosis and sterilized by filtration or autoclave, were inoculated intradermally into Wistar rats at concentrations of 0.25, 0.5 and 1 μg/μL, and the effects on their tegument were observed at 24 and 72 hours. After histological procedures and staining by hematoxyline… Show more

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Cited by 7 publications
(7 citation statements)
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References 24 publications
(35 reference statements)
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“…as the most prevalent species and a potent source of biologically active compounds (37,38). Many studies have been carried out on metabolites production from several Fusarium species especially F. oxysporum and F. solani (39)(40)(41)(42). Fusarium equiseti (Nectriaceae) (teleomorph: Gibberella intricans) is a toxigenic species and a soil inhabitant known to cause disease in several plant species.…”
Section: Discussionmentioning
confidence: 99%
“…as the most prevalent species and a potent source of biologically active compounds (37,38). Many studies have been carried out on metabolites production from several Fusarium species especially F. oxysporum and F. solani (39)(40)(41)(42). Fusarium equiseti (Nectriaceae) (teleomorph: Gibberella intricans) is a toxigenic species and a soil inhabitant known to cause disease in several plant species.…”
Section: Discussionmentioning
confidence: 99%
“…Experience of disease outbreaks in zebrafish facility is very critical when considering the precise results from the experiments, operational cost and public health (diseases transmissible to researchers), etc. More importantly, confounding effects of unknown infections or stressors in experimental mammals reportedly changed the research outcome of histology, gene regulation, physiology and immunology (Gentry & Cooper 1981;Hernandes et al 2012). Therefore, identifications of new pathogens in zebrafish systems and knowledge of factors associated with pathogenicity, growth regulatory and inhibitory factors are benefited to overall zebrafish community by improving the fish health and reduction of unwanted variability to further development of the zebrafish as a versatile model organism.…”
Section: Introductionmentioning
confidence: 99%
“…However, it should be noted that during dialysis to obtain the crude extract, molecules with a weight below 12–16 KDa, such as the trichothecenes, (250–500 Da), which are known to cause tissue reaction, whether in the form of inflammation or induction of programmed cell death, were also eliminated. Additionally, some molecules may have been damaged when obtaining the fraction, when the crude extract was heated to approximately 40 °C in a rotaevaporator . The results of TUNEL staining reinforce this hypothesis, as there was no TUNEL‐positive staining in the skin injected with the fraction.…”
Section: Discussionmentioning
confidence: 58%
“…Additionally, some molecules may have been damaged when obtaining the fraction, when the crude extract was heated to approximately 40°C in a rotaevaporator. [17] The results of TUNEL staining reinforce this hypothesis, as there was no TUNEL-positive staining in the skin injected with the fraction. Contrastingly, topically treated skin [18] or skin injected with the crude extract had staining in the epidermis, dermis, and even the subcutaneous tissue.…”
Section: Discussionmentioning
confidence: 58%