Identificação sorológica de espécies de vírus que infetam cucurbitáceas em áreas produtoras do Maranhão

Abstract

Os vírus representam sérios obstáculos para o sucesso da olericultura no mundo inteiro, constituindo a identificação daqueles de maior incidência numa região, papel fundamental para o estabelecimento de estratégias de controle. Visitas de campo foram realizadas a plantios de espécies de cucurbitáceas em áreas produtoras do Maranhão e amostras foliares foram coletadas de 118 plantas com sintomas ou suspeita de sintomas de vírus, sendo 46 de abóbora (Cucurbita moschata), 30 de melancia (Citrullus lanatus), 23 de maxixe (Cucumis anguria), 13 de pepino (C. sativus) e seis de melão (C. melo). Todas as amostras foram testadas contra anti-soros específicos para os principais vírus das famílias Bromoviridae, Comoviridae e Potyviridae que infetam cucurbitáceas no Nordeste, mediante "enzyme-linked immunosorbent assay" (ELISA) indireto e dupla difusão em agar. Os resultados revelaram a identificação sorológica de Papaya ringspot vírus (PRSV) em 64,4% das amostras analisadas, seguido de Watermelon mosaic virus-2 (WMV-2) em 15,2%, Cucumber mosaic virus (CMV) em 6,8%, Squash mosaic virus (SqMV) em 3,4% e Zucchini yellow mosaic virus (ZYMV) em 3,4%. Este levantamento confirma a predominância do PRSV em espécies de cucurbitáceas cultivadas no estado do Maranhão.

Characterization of Cucurbit Production Systems and Disease Prevalence in Municipalities in Pernambuco

“…In the agar doublediffusion test, the squash mosaic virus (SqMV) was not present, either. Studies by Oliveira et al (2000) in northeast Brazil, Moura et al (2001) in the state of Maranhão, Halfeld-Vieira et al (2004) in Roraima and Silveira (2009) in the Lower Basin of the São Francisco river did not show the occurrence of squash in significant amounts in serological survey studies, indicating low or no prevalence of such viruses in the study area.…”

“…ZYMV was identified in a significant degree of intensity: it was present in 42.8%, 50% and 78.6% of the infected samples of watermelons; pumpkins and melons, respectively (Table 2). This virus has been identified and reported in various regions of Brazil, e.g., in Maranhão by Moura et al (2001); Roraima by Halfeld-Vieira et al (2004); in the Lower Basin of the São Francisco river by Silveira et al (2009) and more recently by Alencar et al (2012), who confirmed the presence of this virus in 44% of the leaf samples in a survey on cucurbits in the state of Tocantins.…”

Susceptibility assessment of watermelon and pumpkin cultivars to potyvirus in Tocantins

“…The location has been associated to the virus predominance, since there are different strains of viruses in each region. Thus, in Brazil, PRSV and ZYMV were predominant in the region of São Francisco valley (SILVEIRA et al, 2009), in the state of São Paulo (REGATIER et al, 2008) and Maranhão state (MOURA et al, 2001). According to Halfeld-Vieira et al (2004), in Roraima, there are PRSV-W, WMV and ZYMV in simple and mixed infections.…”

Serology Applied to Plant Virology

“…More than 20 virus species were found naturally infecting different cucurbit species, but only five species were serologically detected and isolated in Northeastern Brazil, including those that belong to the following families and genera: family Bromoviridae (Lima & Amaral, 1985;Moura et al, 2001;.…”