Analysis of the nucleotide sequence of the coat protein and 3'-untranslated region of two Brazilian Potato virus Y isolates

Inoue‐Nagata, Alice K.; Fonseca, M. E. N.; Lobo, Tatiana O.T.A.; Ávila, A. C. de; Monte, D. C.

doi:10.1590/s0100-41582001000100008

Cited by 14 publications

(5 citation statements)

References 17 publications

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“…Therefore, classification of potato isolates of PVY based on the 3 0 -UTR implies that this region cannot be used to demarcate strains of the isolates. Furthermore, it seems the unique 3 0 -UTR sequence of PVY is not related to the tuber or tobacco leaf necrosis symptoms, as also indicated by others [7,25]. One would expect there to be some level of correlation between the serology assays and CP sequences of PVY since the MAbs bind to the CP, however, as we have demonstrated in this paper, there is no direct correlation.…”

Section: Strain Demarcationsupporting

confidence: 57%

Characterisation of potato virus Y isolates from Iran

et al. 2010

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A survey of Potato virus Y (PVY) was conducted in cultivated fields in six Iranian provinces between January 2005 to July 2007. Two hundred samples from potato and tomato were collected and analyzed by enzyme-linked immunosorbent assay (ELISA) for potyviruses. Almost one fourth of the samples were found to be infected by PVY. Analysis of these PVY-positive samples using three monoclonal antibodies (MAbs) facilitating the simultaneous detection of three main strains namely the ordinary (PVY(O)), strain (PVY(N)) and C (PVY(C)) strains. However, the fourth strain (PVY(NTN)) and some others recombinant isolates were also identified by molecular methods. Host range and symptoms analysis using sap inoculation of four different strains of PVY onto a range of plants revealed that the four strains showed biological properties that seemed to be consistent with their molecular grouping. Fourteen isolates of PVY were chosen based on the host and geographical location, primer specificity and serology for further biological and molecular characterisation. The coat protein (CP) and P1 genes and 3'-non-translated region (3'NTR) from 14 representative isolates were sequenced and analysed with the sequences available in GenBank. Composite analysis of the P1, CP and 3'-UTR sequences with all full genome sequences of PVY revealed that there are three potential strains of PVY in Iran, PVY(O), PVY(N)-W and PVY(NTN). Isolate KER.SA(N) was the most divergent of all the 14 isolates reacted with PVY(N) specific MAbs but grouped with PVY(O) strains in maximum likelihood phylogentic analysis. The PVY(NTN) isolates from Iran more closely related to the European than North American PVY(NTN) isolates.

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Section: Strain Demarcationsupporting

confidence: 57%

Characterisation of potato virus Y isolates from Iran

et al. 2010

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“…This is a derivative of PVY N strain, which induces vein necrosis in tobacco leaves and provokes PTNRD and severe chlorotic mosaic in potato leaves (5).…”

Section: Discussionmentioning

confidence: 99%

Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187

2015

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“…However, the composition of the recombinant strains in Brazil has not been studied on a systematic basis and the contribution of mutations and new recombination events occurring in Brazil has not been determined. Among the Brazilian isolates reported to date, there are some with strong similarities to PVY O and PVY N strains and some recombinants similar to PVY NTN (Inoue‐Nagata et al. , 2001; Sawazaki et al.…”

Section: Introductionmentioning

confidence: 99%