A novel type of <i>Potato virus Y</i> recombinant genome, determined for the genetic strain PVY<sup>E</sup>

Galvino-Costa, Suellen Bárbara Ferreira; Figueira, Antônia dos Reis; Camargos, V. V.; Geraldino, P. S.; Hu, XJ; Николаева, О. В.; Kerlan, Camille; Karasev, Alexander V.

doi:10.1111/j.1365-3059.2011.02495.x

Cited by 56 publications

(20 citation statements)

References 32 publications

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“…Эти гены путем селекции переносились в различные культуры картофеля, резуль татом чего стало появление множества резистентных к PVY сортов. Основными разновидностями устойчивых к PVY генотипов являются генотипы, несущие гены Ny, Nc и Nz, которые отвечают за развитие HR, и ген Ry, отвечающий за возникновение ER (Valkonen, 2015 (Singh et al, 2008;GalvinoCosta et al, 2012;Karasev, Gray, 2013). Доминантные вирусспецифичные гены R, вызыва ющие ER, ингибируют развитие вирусной инфекции и эффективно защищают растения картофеля.…”

Section: специфическая устойчивость растений к вирусамunclassified

Resistance to viruses of potato: current status and prospects

Makarova¹,

Макаров²,

Taliansky³

et al. 2017

Vestn. VOGiS

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Section: специфическая устойчивость растений к вирусамunclassified

Resistance to viruses of potato: current status and prospects

Makarova¹,

Макаров²,

Taliansky³

et al. 2017

Vestn. VOGiS

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“…This was verified with PVY NTN specific primers. Thus, it can be excluded that among samples tested there are PVY N Wi strains with genome structures like isolates 156 or Syr-NB-16, or novel PVY molecular genotypes such as isolate NE-11 (Lorenzen et al 2008) or strain PVY E (Galvino-Costa et al 2012). These genotypes cannot be detected using the specific primers mentioned above.…”

Section: Strain Specific Detection Of Pvy Using Conventional Pcrmentioning

confidence: 99%

“…Sections from NE-11 can be found in PVY-MON (accession number FJ204165), which is an isolate of strain PVY E . Therefore, recombinants can be parents for further recombinant genotypes(Galvino-Costa et al 2012). The genome of isolate L26 displays typical features of PVY NTN -type isolates with 3 RJs and was classified as PVY NTN by…”

mentioning

confidence: 99%

Comparison of three methods for the detection of Potato virus Y in seed potato certification

Hühnlein¹,

Drechsler²,

Steinbach³

et al. 2013

J Plant Dis Prot

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Potato virus Y (PVY) is becoming increasingly important in potato growing regions worldwide. The main reason for this is an increase in the incidence of infections with recombinant forms of PVY, such as PVY N Wi and PVY NTN . They are characterized by high virulence and low symptom expression, which is especially true of PVY N Wi. This makes it difficult to detect infected seed potato plants during certification. In Mecklenburg-Western Pomerania (North-East Germany) in 2008 an unusually high incidence of infection with PVY was recorded in fields where seed potatoes were being grown. In this study we examined, which strains of PVY caused these infections. Furthermore, we have developed a reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) assay for direct tuber testing, which we compared to direct tuber testing by ELISA and growing-on tests. As a result, we recommend for direct tuber testing by RT-qPCR or ELISA. These methods are less space-and time-consuming and therefore less costly alternatives to conventional ELISA testing of eye cuttings from seed potatoes. Additionally, the RT-qPCR method has a high efficacy, so that even freshly harvested non-dormant tubers can be tested, which makes testing very fast and economical. This is of special interest in cases when tubers shall be exported to the other hemisphere of the world.

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“…Within a suitable host, the PVY RNA genome is translated into a large precursor polyprotein, which is cleaved into 10 mature proteins (Berger et al, 2005). PVY has a large diversity of strain groups and genetic variants within strains, linked to its biological, serological and molecular properties (Singh et al, 2008;Karasev et al, 2011 (Galvino-Costa et al, 2012). According to their sequences, isolates of the PVY N strain group fall into two genetic subgroups, which are the European (PVY N ) and North American (NA-PVY N ) (Chikh Ali et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Biological, serological and molecular typing of potato virus Y (PVY) isolates from Tunisia

Tayahi

Gharsallah

Khamassy³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. In Tunisia, potato virus Y (PVY) currently presents a significant threat to potato production, reducing tuber yield and quality. Three hundred and eighty-five potato samples (six different cultivars) collected in autumn 2007 from nine regions in Tunisia were tested for PVY infection by DAS-ELISA. The virus was detected in all regions surveyed, with an average incidence of 80.26%. Subsequently, a panel of 82 Tunisian PVY isolates (PVY-TN) was subjected to systematic biological, serological and molecular typing using immunocapture reverse-transcription polymerase chain reaction and a series of PVY OCand PVY N -specific monoclonal antibodies. Combined analyses revealed ~67% of PVY NTN variants of which 17 were sequenced in the 5'NTR-P1 region to assess the genetic diversity and phylogenetic relationship of PVY-TN against other worldwide PVY isolates. To investigate whether selective constraints could act on viral genomic RNA, synonymous and non-synonymous substitution rates and their ratio were analyzed. Averages of all pairwise comparisons obtained in the 5'NTR-P1 region allowed more synonymous changes, suggesting selective constraint acting in this region. Selective neutrality test was significantly negative, suggesting a rapid expansion of PVY isolates. Pairwise mismatch distribution gave a bimodal pattern and pointed to an eventually early evolution characterizing these sequences. Genetic haplotype network topology provided evidence of the existence of a distinct geographical structure. This is the first report of such genetic analyses conducted on PVY isolates from Tunisia.

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A novel type of Potato virus Y recombinant genome, determined for the genetic strain PVY^E

Cited by 56 publications

References 32 publications

Resistance to viruses of potato: current status and prospects

Resistance to viruses of potato: current status and prospects

Comparison of three methods for the detection of Potato virus Y in seed potato certification

Biological, serological and molecular typing of potato virus Y (PVY) isolates from Tunisia

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A novel type of Potato virus Y recombinant genome, determined for the genetic strain PVYE

Cited by 56 publications

References 32 publications

Resistance to viruses of potato: current status and prospects

Resistance to viruses of potato: current status and prospects

Comparison of three methods for the detection of Potato virus Y in seed potato certification

Biological, serological and molecular typing of potato virus Y (PVY) isolates from Tunisia

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Product

Resources

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A novel type of Potato virus Y recombinant genome, determined for the genetic strain PVY^E