Characterisation of potato virus Y isolates from Iran

Hosseini, Atefe; Massumi, Hossain; Heydarnejad, Jahangir; Pour, Akbar Hosseini; Varsani, Arvind

doi:10.1007/s11262-010-0546-8

Cited by 12 publications

(9 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our analysis revealed that PVY O and PVY NTN were predominant strains in Iran. Comparing results of this study with those published earlier (Pourrahim et al, 2007;Hosseini et al, 2011) suggested that during the past decade the prevalence of PVY NTN was signifi cantly increased within Iranian PVY population.…”

Section: Discussionsupporting

confidence: 70%

See 1 more Smart Citation

Comparative genetic diversity of potato virus Y populations based on coat protein gene

Hosseini¹,

Mehrvar²,

Zakiaghl³

et al. 2017

View full text Add to dashboard Cite

Potato virus Y (PVY) is an important plant pathogen with a wide host range including economically important crops of potato, tobacco, tomato, and pepper. The coat protein gene has been commonly used in studying molecular biology of plant viruses including PVY. In this study, we used a large dataset of CP sequences from isolates collected across the world to assess the detailed molecular evolution of PVY populations with a focus on the Iranian PVY population. Phylogenetic analysis showed that the world PVY population had two major lineages (O:C and N:NTN); each comprising several divergent sublineages. Results showed that the Iranian PVY isolates were distributed across the tree suggesting polyphyletic origin of the Iranian PVY population. Statistical analysis revealed great genetic differences between pairs of the PVY phylogenetic populations. Host populations and also geographical populations of PVY were genetically differentiated. The extent of the genetic diversification among PVY host and geographical populations were mild or moderate. Purifying selection was detected on the CP gene sequences of the PVY populations, suggesting that most of the mutations in the gene were harmful, thereby were eliminated by natural selection. We also detected a variety of recombination patterns to occur along the CP gene of the PVY strains. A significant number of the Iranian PVY isolates were found to be recombinant. Different analyses suggest that Iranian PVY population is highly diverse. In conclusion, results of this study demonstrated that different factors including mutation, host adaptation, geographical distinction and selection pressure shaped the genetic structure of the PVY populations.

show abstract

Section: Discussionsupporting

confidence: 70%

“…Based on the host range, symptomatology and serological traits, PVY O , PVY NW , PVY C , PVY N and PVY NTN strains have been recognized in potato fi elds of Iran. Furthermore, the sequence of P1, coat protein (CP) and 3' UTR of some of the Iranian PVY isolates has been reported (Hosseini et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Comparative genetic diversity of potato virus Y populations based on coat protein gene

Hosseini¹,

Mehrvar²,

Zakiaghl³

et al. 2017

View full text Add to dashboard Cite

show abstract

“…and the use of non-certified virus infected seed. In current study, the ordinary strain of PVY was detected and there is need for further investigations of different PVY isolates as its epidemiology is directly related to different strains ( Hosseini et al, 2011 ). PVA infection is often symptomless in most of the potato cv.…”

Section: Discussionmentioning

confidence: 98%

Detection of Multiple Potato Viruses in the Field Suggests Synergistic Interactions among Potato Viruses in Pakistan

Hameed

Iqbal

Asad

et al. 2014

The Plant Pathology Journal

View full text Add to dashboard Cite

Viral diseases have been a major limiting factor threating sustainable potato (Solanum tuberosum L.) production in Pakistan. Surveys were conducted to serologically quantify the incidence of RNA viruses infecting potato; Potato virus X (PVX), Potato virus Y (PVY), Potato virus S (PVS), Potato virus A (PVA), Potato virus M (PVM) and Potato leaf roll virus (PLRV) in two major potato cultivars (Desiree and Cardinal). The results suggest the prevalence of multiple viruses in all surveyed areas with PVY, PVS and PVX dominantly widespread with infection levels of up to 50% in some regions. Co-infections were detected with the highest incidence (15.5%) for PVX and PVS. Additionally the data showed a positive correlation between co-infecting viruses with significant increase in absorbance value (virus titre) for at least one of the virus in an infected plant and suggested a synergistic interaction. To test this hypothesis, glasshouse grown potato plants were challenged with multiple viruses and analyzed for systemic infections and symptomology studies. The results obtained conclude that multiple viral infections dramatically increase disease epidemics as compared to single infection and an effective resistance strategy in targeting multiple RNA viruses is required to save potato crop.

show abstract

“…A few studies have dealt with PVY from Iran (20), but to the best of our knowledge there is no report concerning expression of the virus CP gene in E. coli. Analysis showed that the CP can be efficiently expressed via pET21a (+) in E. coli (Figure 2A).…”

Section: Discussionmentioning

confidence: 99%