Preparative separation of C19-diterpenoid alkaloids from Aconitum carmichaelii Debx by pH‑zone-refining counter-current chromatography

Abstract

Recebido em 28/3/13; aceito em 27/6/13; publicado na web em 21/8/13The technique of pH-zone-refining counter-current chromatography was successfully applied to preparatively separate three C 19 -diterpenoid alkaloids from the crude extracts of Aconitum carmichaelii for the first time using a two-phase solvent system of petroleum ether-ethyl acetate-methanol-water (5:5:1:9, v/v/v/v). Mesaconitine (I), hypaconitine (II), and deoxyaconitine (III) were obtained from 2.5 g of the crude alkaloids in a one-step separation; the yields were 4.16%, 16.96%, and 5.05%, respectively. The purities of compounds I, II, and III were 93.0%, 95%, and 96%, respectively, as determined by HPLC. The chemical structures of the three compounds were identified by electrospray ionization mass spectrometry (ESI-MS) and NMR.

UPLC–Q‐TOF–HDMS Analysis of Constituents in the Root of Two Kinds of Aconitum Using a Metabolomics Approach

“…At present, a comparison of metabolic profiles has revealed that three major groups of alkaloidsamine diterpenoid alkaloids (ADAs), diester diterpene alkaloids (DDAs), and lipoalkaloidsare present in the roots of Aconitum. They share a common C19-norditerpenoid skeleton, in which the C8 position is occupied by a hydroxyl, an acetoxy or a fatty acid acyl group, respectively (Hu et al, 2009;Liu et al, 2011;Xu et al, 2011;Kawasaki et al, 2011). The toxicity of Aconitum derives mainly from the DDAs, including aconitine, mesaconitine and hypaconitine (Lu et al, 2010).…”

Separation and purification of five alkaloids from Aconitum duclouxii by counter‐current chromatography

“…More recently, three C 19 -diterpenoid alkaloids were isolated from the lateral roots of Aconitum carmichaeli Debx by CCC [27]. However, there is no article gthus far reporting the separation of C 19 -diterpenoid alkaloids from A. duclouxii by CCC.…”

“…To the best of our knowledge, many studies have been published, reporting the successful use of CCC for the separation and purification of C 20 ‐ and C 18 ‐diterpenoid alkaloids from genus aconitum . More recently, three C 19 ‐diterpenoid alkaloids were isolated from the lateral roots of Aconitum carmichaeli Debx by CCC . However, there is no article gthus far reporting the separation of C 19 ‐diterpenoid alkaloids from A. duclouxii by CCC.…”