Utilização de detecção por espalhamento de luz evaporativo para a análise de produtos naturais

Abstract

Recebido em 13/9/11; aceito em 14/12/11; publicado na web em 23/3/12 EVAPORATIVE LIGHT-SCATTERING DETECTOR FOR ANALYSIS OF NATURAL PRODUCTS. The interest in the use of evaporative light scattering detector (ELSD) for the analysis of different classes of natural products has grown over the years. This is because this detector has become an excellent alternative compared to other types of detectors, such as the refractive index detector and the ultraviolet (UV) detector. This review describes the basic principles of ELSD functioning and discusses the advantages and disadvantages in using an ELSD for the analysis of organic compounds. Additionaly, an overview, covering the last 23 years, of ELSD applications in natural products analysis (saponins, terpenes, carbohydrates, glycosides, alkaloids, steroids, flavonoids, peptides, polyketides, coumarins and iridoids) is presented and discussed.

Carbohydrate analysis: From sample preparation to HPLC on different stationary phases coupled with evaporative light‐scattering detection

“…However, it is very important to use the lowest possible temperature in this process to obtain a larger particle size and thereby a higher intensity of scattered light. Furthermore, a high temperature could also lead to a loss of sensitivity by partial sublimation of the analytes .…”

Phytochemical screening and analgesic profile of the lyophilized aqueous extract obtained from Chrysobalanus icaco leaves in experimental protocols

“…This evidence is viewed in the UV spectra (Figure 2(A-D)). Absorption spectra in the UV lower than 200 nm are characteristic the series of sesquiterpenes and diterpenes, especially when seen coupled to photodiode detector (Kong et al 2001;Urano et al 2012).…”